Transcriptomic Analysis of the Halostachys caspica in Response to Short-term Salt Stress

doi:10.7525/j.issn.1673-5102.2018.01.011

Abstract

Abstract: As a halophyte with strong salt tolerance, Halostachys caspica widely distributes in desert saline-alkali land. In order to reveal the genomic changes of gene expression under salt stress, transcriptome sequencing of H.caspica assimilating branches with treatments of 300 and 500 mmol·L^-1 NaCl for 3 h was performed. A total of 153 298 unigenes with an average length of 643 bp were obtained with clean reads assembled. The 47 subclasses and 118 KEGG pathways were enriched in the GO terms and KEGG pathways, respectively. Differentially expressed genes analysis showed that there were 4 432 and 2 580 unigenes in response to low salt (300 mmol·L^-1) and high salt (500 mmol·L^-1) in short-term stress, respectively. The 1 245 unigenes were the common differentially expressed genes in the two salt stresses. They were mainly enriched in cellular process, metabolic process and response to stimulus. The osmotic regulation and reactive oxygen species (ROS) scavenging genes were screened out, and most of them were up-regulated. Therefore, H.caspica could improve short-term salt stress adaptation by enhancing the osmotic adjustment and ROS scavenging.

Key words: Halostachys caspica, salt stress, transcriptome, differentially expressed genes, osmotic adjustment, reactive oxygen species

CLC Number:

S687

ZHANG Li-Li, ZHANG Fu-Chun. Transcriptomic Analysis of the Halostachys caspica in Response to Short-term Salt Stress[J]. Bulletin of Botanical Research, 2018, 38(1): 91-99.

References

1. Zhu J K. Plant salt tolerance[J]. Trends in Plant Science,2001,6(2):66-71.
2. Hasegawa P M,Bressan R A,Zhu J K,et al. Plant cellular and molecular responses to high salinity[J]. Annual Review of Plant Physiology and Plant Molecular Biology,2000,51:463-499.
3. Sahu B B,Shaw B P. Isolation,identification and expression analysis of salt-induced genes in Suaeda maritima,a natural halophyte,using PCR-based suppression subtractive hybridization[J]. BMC Plant Biology,2009,9:69.
4. Ventura Y,Eshel A,Pasternak D,et al. The development of halophyte-based agriculture:past and present[J]. Annals of Botany,2014,115(3):529-540.
5. 郗金标,张福锁,毛达如,等. 新疆盐生植物群落物种多样性及其分布规律的初步研究[J]. 林业科学,2006,42(10):6-12.Xi J B,Zhang F S,Mao D R,et al. Species diversity and distribution of halophytic vegetation in Xinjiang[J]. Scientia Silvae Sinicae,2006,42(10):6-12.
6. Kumari S,Sabharwal V P N,Kushwaha H R,et al. Transcriptome map for seedling stage specific salinity stress response indicates a specific set of genes as candidate for saline tolerance in Oryza sativa L. [J]. Functional & Integrative Genomics,2009,9(1):109-123.
7. Xie Q,Niu J,Xu X L,et al. De novo assembly of the Japanese lawngrass(Zoysia japonica Steud.) root transcriptome and identification of candidate unigenes related to early responses under salt stress[J]. Frontiers in Plant Science,2015,6:610.
8. Jin H X,Dong D K,Yang Q H,et al. Salt-responsive transcriptome profiling of Suaeda glauca via RNA sequencing[J]. PLoS One,2016,11(3):e0150504.
9. 杜驰,张冀,张富春. 盐胁迫诱导盐穗木Rev1、Rev3基因的表达分析[J]. 植物研究,2017,37(2):211-215,226.Du C,Zhang J,Zhang F C. Expression analysis of Rev1 and Rev3 of Halostachys caspica under salt stress[J]. Bulletin of Botanical Research,2017,37(2):211-215,226.
10. Türkan I,Demiral T. Recent developments in understanding salinity tolerance[J]. Environmental and Experimental Botany,2009,67(1):2-9.
11. Deinlein U,Stephan A B,Horie T,et al. Plant salt-tolerance mechanisms[J]. Trends in Plant Science,2014,19(6):371-379.
12. Benhassaine-kesri G,Aid F,Demandre C,et al. Drought stress affects chloroplast lipid metabolism in rape(Brassica napus) leaves[J]. Physiologia Plantarum,2002,115(2):221-227.
13. Konova I V,Sergeeva Y E,Galanina L A,et al. Lipid synthesis by Geomyces pannorum under the impact of stress factors[J]. Microbiology,2009,78(1):42-47.
14. Miled D D B,Zarrouk M,Chérif A. Sodium chloride effects on lipase activity in germinating rape seeds[J]. Biochemical Society Transactions,2000,28(6):899-902.
15. Stitt M,Sulpice R,Keurentjes J. Metabolic networks:how to identify key components in the regulation of metabolism and growth[J]. Plant Physiology,2010,152(2):428-444.
16. Ritala A,Dong L M,Imseng N,et al. Evaluation of tobacco(Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol,the first committed step in terpenoid indole alkaloid pathway[J]. Journal of Biotechnology,2014,176:20-28.
17. Li C Y,Leopold A L,Sander G W,et al. The ORCA2 transcription factor plays a key role in regulation of the terpenoid indole alkaloid pathway[J]. BMC Plant Biology,2013,13(1):155.
18. 赵航,贾富强,张富春,等. 盐胁迫下盐穗木差异表达基因的转录组信息分析[J]. 生物信息学,2014,12(2):90-98.Zhao H,Jia F Q,Zhang F C,et al. The transcriptome information analysis of differentially expressed genes of Halostachys caspica under salt stress[J]. Chinese Journal of Bioinformatics,2014,12(2):90-98.
19. 黎裕. 植物的渗透调节与其它生理过程的关系及其在作物改良中的应用[J]. 植物生理学通讯,1994,30(5):377-383.Li Y. The relationship between osmotic adjustment and other physiological processes and its application in crop improvement[J]. Plant Physiology Communications,1994,30(5):377-383.
20. Hanson A D,Nelsen C E,Pedersen A R,et al. Capacity for proline accumulation during water stress in barley and its implications for breeding for drought resistance[J]. Crop Science,1979,19(4):489-493.
21. Patakas A,Nikolaou N,Zioziou E,et al. The role of organic solute and ion accumulation in osmotic adjustment in drought-stressed grapevines[J]. Plant Science,2002,163(2):361-367.
22. 王娟,李德全. 逆境条件下植物体内渗透调节物质的积累与活性氧代谢[J]. 植物学通报,2001,18(4):459-465.Wang J,Li D Q. The accumulation of plant osmoticum and activated oxygen metabolism under stress[J]. Chinese Bulletin of Botany,2001,18(4):459-465.
23. Caprioli M,Katholm A K,Melone G,et al. Trehalose in desiccated rotifers:a comparison between a bdelloid and a monogonont species[J]. Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2004,139(4):527-532.
24. Zhang H M,Murzello C,Sun Y,et al. Choline and osmotic-stress tolerance induced in Arabidopsis by the soil microbe Bacillus subtilis(GB03)[J]. Molecular Plant-Microbe Interactions,2010,23(8):1097-1104.
25. Suzuki N,Koussevitzky S,Mittler R,et al. ROS and redox signalling in the response of plants to abiotic stress[J]. Plant,Cell & Environment,2012,35(2):259-270.
26. Gill S S,Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J]. Plant Physiology and Biochemistry,2010,48(12):909-930.
27. Jaleel C A,Riadh K,Gopi R,et al. Antioxidant defense responses:physiological plasticity in higher plants under abiotic constraints[J]. Acta Physiologiae Plantarum,2009,31(3):427-436.
28. Chen S,Polle A. Salinity tolerance of Populus[J]. Plant Biology,2010,12(2):317-333.
29. Vaidyanathan H,Sivakumar P,Chakrabarty R,et al. Scavenging of reactive oxygen species in NaCl-stressed rice(Oryza sativa L.)-differential response in salt-tolerant and sensitive varieties[J]. Plant Science,2003,165(6):1411-1418.

[1]	Yajie LIU, Lizhe AN. Nitric Oxide Mediates Brassinosteroids-induced Chilling Tolerance in Chorispora bungeana Suspension Cultured Cells [J]. Bulletin of Botanical Research, 2024, 44(1): 118-131.
[2]	Fazhi FANG, Huiying GUI, Zhaojia LI, Xiaofeng ZHANG. Physiological Adaptation of Six Mangrove Seedlings to Different Salinity [J]. Bulletin of Botanical Research, 2023, 43(6): 881-889.
[3]	Lei XU, Xiao XU, Qinsong LIU. Effects of Exogenous Salicylic Acid on Antioxidant System and Gene Expression of Davidia involucrata Seedlings under Salt Stress [J]. Bulletin of Botanical Research, 2023, 43(4): 572-581.
[4]	Zhanmin ZHENG, Yubing SHANG, Guangbo ZHOU, Di XIAO, Yi LIU, Xiangling YOU. Genetic Transformation and Function Analysis of PsnHB13 and PsnHB15 of Populus simonii × Populus nigra [J]. Bulletin of Botanical Research, 2023, 43(3): 340-350.
[5]	Li LI, Xin WANG, Yuejing ZHANG, Lingyun JIA, Hailong PANG, Hanqing FENG. Effects of Abiotic Stresses on the Intracellular and Extracellular ATP Levels of Tobacco Suspension Cells [J]. Bulletin of Botanical Research, 2023, 43(2): 179-185.
[6]	Shixian LIAO, Yuting WANG, Liben DONG, Yongmei GU, Fenglin JIA, Tingbo JIANG, Boru ZHOU. Function Analysis of the Transcription Factor PsnbZIP1 of Populus simonii×P. nigra in Response to Salt Stress [J]. Bulletin of Botanical Research, 2023, 43(2): 288-299.
[7]	Senyao LIU, Fenglin JIA, Qing GUO, Gaofeng FAN, Boru ZHOU, Tingbo JIANG. Response Analysis of Transcription Factor PsnbHLH162 Gene in Populus simonii × P. nigra under Salt Stress and Low Temperature Stress [J]. Bulletin of Botanical Research, 2023, 43(2): 300-310.
[8]	Hong YANG, Lifeng WANG, Longjun DAI, Bingbing GUO. Effects of Tapping Panel Dryness on Mitochondrial Ultrastructure and ROS Metabolism in Barks of Rubber Tree (Hevea brasiliensis) [J]. Bulletin of Botanical Research, 2023, 43(1): 69-75.
[9]	Liran YUE, Yingjie LIU, Chenxu LIU, Yunwei ZHOU. Cloning and Functional Analysis of miR398a from Chrysanthemum× grandiflora in Response to Salt Stress [J]. Bulletin of Botanical Research, 2022, 42(6): 986-996.
[10]	Denggao LI, Rui LIN, Qinghui MU, Na ZHOU, Yanru ZHANG, Wei BAI. Cloning and Functional Analysis of StNPR4 gene in Solanum tuberosum [J]. Bulletin of Botanical Research, 2022, 42(5): 821-829.
[11]	Jiaorao CHEN, Xu XU, Zhangli HU, Shuang YANG. Recent Advances on Salt Stress Sensitivity and Related Calcium Signals in Plants [J]. Bulletin of Botanical Research, 2022, 42(4): 713-720.
[12]	He CHENG, Shuanghui TIAN, Yang ZHANG, Cong LIU, De’an XIA, Zhigang WEI. Genome-wide Identification and Expression Analysis of nsLTP Gene Family in Populus trichocarpa [J]. Bulletin of Botanical Research, 2022, 42(3): 412-423.
[13]	Dongmei HUANG, Ying CHEN, Lu BAI, Di’an NI, Yiyang XU, Zhiguo ZHANG, Qiaoping QIN. Transcriptome Analysis of Hemerocallis fulva Leaves Respond to Low Temperature Stress [J]. Bulletin of Botanical Research, 2022, 42(3): 424-436.
[14]	Jianxin LIU, Ruirui LIU, Xiuli LIU, Haiyan JIA, Ting BU, Na LI. Effects of Spraying NaHS at Different Growth Stages on H₂S Production and Reactive Oxygen Species Metabolism of Naked Oat Leaves under Saline-Alkali Stress [J]. Bulletin of Botanical Research, 2022, 42(3): 455-465.
[15]	Jingya Yu, Mingze Xia, Hao Xu, Faqi Zhang. Comparative Transcriptome Analysis of Three Artemisia Species in Qinghai Tibet Plateau [J]. Bulletin of Botanical Research, 2022, 42(2): 200-210.