Cloning and Functional Analysis of StNPR4 gene in Solanum tuberosum

doi:10.7525/j.issn.1673-5102.2022.05.013

Abstract

Abstract:

In order to clarify the function of StNPR4 in potato， the CDS and promoter sequence of StNPR4 were cloned and bioinformatics analysis was conducted. Tissue expression pattern was performed by qRT-PCR， and a binary expression vector of StNPR4 driven by its own promoter was constructed and transformed into potato. Transgenic plants were used to examine the responses to salicylic acid， Phytophthora infestans and high salt stress respectively. The results showed that StNPR4 had typical functional domains in NPR1 family with cis-acting elements on the promoter in response to biotic and abiotic stresses. The StNPR4 expression level was highest in leaves StNPR4 was inductively expressed by SA and the induced expression was higher in transgenic plants than in the control. StNPR4 transgenic potato plants showed enhanced resistance to Ph. infestans and higher rooting rate under high salt stress. This indicated that StNPR4 played an important role not only in biotic stress but also in abiotic stress of potato.

Key words: StNPR4, cloning, genetic transformation, Phytophthora infestans, salt stress, potato

CLC Number:

Q939.92

Denggao LI, Rui LIN, Qinghui MU, Na ZHOU, Yanru ZHANG, Wei BAI. Cloning and Functional Analysis of StNPR4 gene in Solanum tuberosum[J]. Bulletin of Botanical Research, 2022, 42(5): 821-829.

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