Cloning and Functional Analysis of miR398a from Chrysanthemum× grandiflora in Response to Salt Stress

doi:10.7525/j.issn.1673-5102.2022.06.008

Abstract

Abstract:

miRNAs played an important role in abiotic stress， and miR398a and pre-miR398a sequences were obtained from Small RNA database of Chrysanthemum×grandiflora. The sequence alignment showed that the cgr-miR398a was highly conserved with the identified miR398a sequences of other plants. Target genes prediction of cgr-miR398a showed that their annotation of targets include superoxide dismutase［Cu-Zn］（CSD2）， copper chaperone（CCS）， A20/AN1-like zinc finger family protein（SAP8）. The qPCR assay showed that the expression levels of miR398a was significantly decreased while target genes showed contrary trend during salt stress in Chrysanthemum×grandiflora. To explore the function of cgr-mir398a in response to salt stress， and an overexpression vector of cgr-mir398a was constructed and transformedinto Arabidopsis thaliana.The result showed that overexpression of the cgr-MIR398a in Arabidopsis could decrease seed germination under salt stress and salt tolerance at the adult seedling stage， therefore cgr-miR398a played a negative regulatory role in Arabidopsis in response to salt stress. The results provided a valuable reference for further exploring the functional mechanism of miR398a during salt stress in Chrysanthemum×grandiflora.

Key words: Chrysanthemum×grandiflora, cgr-miR398a, target gene, qPCR, salt stress

CLC Number:

S682.1+1

Liran YUE, Yingjie LIU, Chenxu LIU, Yunwei ZHOU. Cloning and Functional Analysis of miR398a from Chrysanthemum× grandiflora in Response to Salt Stress[J]. Bulletin of Botanical Research, 2022, 42(6): 986-996.

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