Table 1

Primer sequence

Fig.1

Identification of 84K poplar PagPYL4 gene and phenotype analysis of the transgenic linesA. Phylogenetic tree analysis of PYL4； B. Semi-quantitative PCR electrophoretogram of transgenic 84K poplar； C. Plant height statistics； D. Ground diameter statistics； E. Phenotypic diagram of transgenic 84K poplar. In Figs. 1C and D， two-way analysis of variance（ANOVA） was used. Data were expressed as mean±SE； the error line represented the standard error of three biological replicates， different lowercase letters indicated significant differences between lines at P<0.05.

Fig.2

Photosynthetic parameters of 84K poplar lines under different irradiancesThe error lines represented standard error of three biological replicates，different lowercase letters indicated significant differences between lines at P<0.05.

Fig.3

Stomatal aperture indexs of different lines under different ABA concentrationsA. The stomatal opening before and after ABA treatment； B. Stomatal aperture after 5 µmol·L-1 ABA treatment for 0 and 2 h； C. Stomatal aperture after 10 µmol·L-1 ABA treatment for 0 and 2 h. OS. Open solution buffer.Different lowercase letters indicated significant differences between lines at P<0.05.

Fig.4

Analysis of drought-resistant physiological indices of 84K poplar linesA. Determination of the water loss rate of detached leaves； B. DAB staining under mannitol treatment； C. The level of DAB staining under mannitol treatment. Different lowercase letters indicated significant differences between 84K poplar lines at P<0.05.

Fig.5

Photosynthetic parameters and phenotypic analysis of 84K poplar lines under short-term drought stressA. Phenotypic observation under short-term drought stress； B. Net photosynthetic rate； C. Stomatal conductance； D. Transpiration rate. Different lowercase letters indicated significant differences between 84K poplar lines at P<0.05.