Response of Pinus Koraiensis Growth and Rhizosphere Soil Enzyme Activity to Lepista nuda Inoculation

doi:10.7525/j.issn.1673-5102.2025.03.015

Abstract

Abstract:

This study investigated the effects of Lepista nuda inoculation at different doses on the growth of Pinus koraiensis seedlings and rhizosphere soil enzyme activity， aiming to provide technical support for cultivating mycorrhizal P. koraiensis seedlings and forestation. Four-year-old P. koraiensis seedlings were treated with four doses of L. nuda inoculant. Growth parameters， soil nutrient content， and rhizosphere soil enzyme activity were measured and analyzed. Correlation analysis was conducted to elucidate the responses of seedling growth and soil enzyme activity to L. nuda colonization. Key findings were that：（1）L. nuda colonization significantly enhanced seedling growth， at an optimal dose of 150 g⋅plant^-1， plant height， ground diameter， fresh weight， and dry weight increased by 29.07%， 25.69%， 38.16%， and 57.44%， respectively， compared to the control. Mycorrhizal colonization rate and root activity increased significantly in the inoculation treatment compared to the control. （2）Inoculation treatments exhibited higher soil nutrient content than the control， at a dose of 150 g⋅plant^-1， the content of soil organic matter， available nitrogen， total nitrogen， total phosphorus， and available phosphorus increased by 60.67%， 31.46%， 35.23%， 72.22%， and 35.91%， respectively. （3）All doses of L. nuda inoculationenhanced rhizosphere soil enzyme activity， with the maximum increment at a dose of 150 g⋅plant^-1. （4）Correlation analysis revealed significant positive relationships between mycorrhizal colonization rate and root activity， biomass（dry/fresh weight）， growth parameters（height/diameter）， the contents of soil organic matter， total nitrogen/phosphorus， available nitrogen， and activities of sucrase， urease， and phosphatase. Soil available phosphorus content was positively correlated with phosphatase activity， while available nitrogen content was positively correlated with content of soil organic matter， and activities of urease and phosphatase. In conclusion， L. nuda inoculation markedly promoted P. koraiensis growth by regulating soil enzyme activity and nutrient cycling， with 150 g⋅plant^-1 identified as the optimal inoculation dose. The findings of this study provided valuable references for the cultivation of mycorrhizal P. koraiensis seedlings and improvement of forest soil quality.

Key words: Lepista nuda, Pinus koraiensis, growth indicators, nutrient content, soil enzyme activity

CLC Number:

Q939.95

Yanyan PAN, Xiaoguang LI, Lijun LIU, Hong LI, Xin ZHONG, Yifei ZHANG. Response of Pinus Koraiensis Growth and Rhizosphere Soil Enzyme Activity to Lepista nuda Inoculation[J]. Bulletin of Botanical Research, 2025, 45(3): 460-470.

Figures/Tables 6

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处理 Treatment	苗高 Height of seedling/cm	地径 Diameter at ground level/cm	鲜质量 Fresh weight/g	干质量 Dry weight/g	干鲜质量比 Ratio of dry weight to fresh weight	侵染率 Colonization rate/%	根系活力 Root viability/%
CK	16.41±1.35^c	4.71±0.37^b	4.14±0.72^c	1.95±0.31^c	0.47±0.05^b	4.38±0.78^c	44.56±4.76^c
M1	18.91±1.11^b	5.64±0.20^a	4.84±0.63^b	2.43±0.21^b	0.51±0.05^a	60.13±5.04^b	62.11±3.71^a
M2	19.44±1.27^b	5.74±0.14^a	5.29±0.35^a	2.64±0.36^b	0.50±0.05^a	67.50±9.21^ab	59.30±6.22^a
M3	21.18±1.87^a	5.92±0.46^a	5.72±0.39^a	3.07±0.22^a	0.54±0.06^a	72.27±5.12^a	60.76±6.76^a
M4	21.15±1.25^a	5.91±0.27^a	5.17±0.27^a	3.05±0.21^a	0.53±0.04^a	72.25±2.85^a	55.02±4.33^b

处理 Treatment	有机质 Soil organic matter/ （g·kg^-1）	碱解氮 Alkali-hydrolyzable nitrogen/ （mg·kg^-1）	全氮 Total nitrogen/ （g·kg^-1）	全磷 Total phosphorus/ （g·kg^-1）	速效磷 Available phosphorous/ （mg·kg^-1）
CK	21.69±1.26^c	122.19±1.73^b	7.92±0.15^b	0.18±0.01^b	5.18±0.07^b
M1	28.34±2.36^b	124.48±2.86^b	8.25±0.17^b	0.20±0.01^b	5.96±0.44^b
M2	33.63±1.76^a	155.83±4.66^a	9.92±0.07^a	0.26±0.01^a	6.87±0.27^a
M3	34.85±0.68^a	160.63±3.73^a	10.71±0.42^a	0.31±0.01^a	7.04±0.24^a
M4	34.35±1.15^a	158.36±3.56^a	10.52±025^a	0.28±0.02^a	6.79±0.25^a

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