Comparative metabolite analysis of Piper sarmentosum organs approached by LC-MS-based metabolic profiling

doi:10.1007/s13659-024-00453-z

Natural Products and Bioprospecting ›› 2024, Vol. 14 ›› Issue (4): 30-30.DOI: 10.1007/s13659-024-00453-z

• ORIGINAL ARTICLES • Previous Articles Next Articles

Comparative metabolite analysis of Piper sarmentosum organs approached by LC-MS-based metabolic profiling

Ismail Ware^1,2,3, Katrin Franke^1,4,5, Andrej Frolov¹, Kseniia Bureiko¹, Elana Kysil¹, Maizatulakmal Yahayu³, Hesham Ali El Enshasy^3,6, Ludger A. Wessjohann^1,5

1. Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany;
2. Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400 Sabah, Malaysia;
3. Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), 81310 Johor Bahru, Johor, Malaysia;
4. Institute of Biology/Geobotany and Botanical Garden, Martin Luther University Halle-Wittenberg, 06108 Halle (Saale), Germany;
5. German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, 04103 Leipzig, Germany;
6. City of Scientific Research and Technology Applications, New Borg Al Arab, Alexandria 21934, Egypt

Received:2024-02-28 Online:2024-08-01
Contact: Katrin Franke,E-mail:kfranke@ipb-halle.de;Ludger A. Wessjohann,E-mail:wessjohann@ipb-halle.de
Supported by:
This study was supported by the German Academic Exchange Service (DAAD) as a grant scholarship and part of the Ph.D. thesis of IW. Funding program/-ID: Research Grants - Doctoral Programs in Germany, 2017/18 (57299294), ST34.

Comparative metabolite analysis of Piper sarmentosum organs approached by LC-MS-based metabolic profiling

Ismail Ware^1,2,3, Katrin Franke^1,4,5, Andrej Frolov¹, Kseniia Bureiko¹, Elana Kysil¹, Maizatulakmal Yahayu³, Hesham Ali El Enshasy^3,6, Ludger A. Wessjohann^1,5

1. Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany;
2. Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400 Sabah, Malaysia;
3. Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), 81310 Johor Bahru, Johor, Malaysia;
4. Institute of Biology/Geobotany and Botanical Garden, Martin Luther University Halle-Wittenberg, 06108 Halle (Saale), Germany;
5. German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, 04103 Leipzig, Germany;
6. City of Scientific Research and Technology Applications, New Borg Al Arab, Alexandria 21934, Egypt

通讯作者: Katrin Franke,E-mail:kfranke@ipb-halle.de;Ludger A. Wessjohann,E-mail:wessjohann@ipb-halle.de
基金资助:
This study was supported by the German Academic Exchange Service (DAAD) as a grant scholarship and part of the Ph.D. thesis of IW. Funding program/-ID: Research Grants - Doctoral Programs in Germany, 2017/18 (57299294), ST34.

Abstract

Abstract: Piper sarmentosum Roxb. (Piperaceae) is a traditional medicinal and food plant widely distributed in the tropical and subtropical regions of Asia, offering both health and culinary benefits. In this study the secondary metabolites in different organs of P. sarmentosum were identified and their relative abundances were characterized. The metabolic profiles of leaves, roots, stems and fruits were comprehensively investigated by liquid chromatography high-resolution mass spectrometry (LC-HR-MS) and the data subsequently analyzed using multivariate statistical methods. Manual interpretation of the tandem mass spectrometric (MS/MS) fragmentation patterns revealed the presence of 154 tentatively identified metabolites, mostly represented by alkaloids and flavonoids. Principle component analysis and hierarchical clustering indicated the predominant occurrence of flavonoids, lignans and phenyl propanoids in leaves, aporphines in stems, piperamides in fruits and lignan-amides in roots. Overall, this study provides extensive data on the metabolite composition of P. sarmentosum, supplying useful information for bioactive compounds discovery and patterns of their preferential biosynthesis or storage in specific organs. This can be used to optimize production and harvesting as well as to maximize the plant’s economic value as herbal medicine or in food applications.

Key words: Piper sarmentosum, LC-MS/MS, Multivariate analysis, Metabolite profiling, Secondary metabolites, Alkaloids

摘要： Piper sarmentosum Roxb. (Piperaceae) is a traditional medicinal and food plant widely distributed in the tropical and subtropical regions of Asia, offering both health and culinary benefits. In this study the secondary metabolites in different organs of P. sarmentosum were identified and their relative abundances were characterized. The metabolic profiles of leaves, roots, stems and fruits were comprehensively investigated by liquid chromatography high-resolution mass spectrometry (LC-HR-MS) and the data subsequently analyzed using multivariate statistical methods. Manual interpretation of the tandem mass spectrometric (MS/MS) fragmentation patterns revealed the presence of 154 tentatively identified metabolites, mostly represented by alkaloids and flavonoids. Principle component analysis and hierarchical clustering indicated the predominant occurrence of flavonoids, lignans and phenyl propanoids in leaves, aporphines in stems, piperamides in fruits and lignan-amides in roots. Overall, this study provides extensive data on the metabolite composition of P. sarmentosum, supplying useful information for bioactive compounds discovery and patterns of their preferential biosynthesis or storage in specific organs. This can be used to optimize production and harvesting as well as to maximize the plant’s economic value as herbal medicine or in food applications.

关键词: Piper sarmentosum, LC-MS/MS, Multivariate analysis, Metabolite profiling, Secondary metabolites, Alkaloids

Ismail Ware, Katrin Franke, Andrej Frolov, Kseniia Bureiko, Elana Kysil, Maizatulakmal Yahayu, Hesham Ali El Enshasy, Ludger A. Wessjohann. Comparative metabolite analysis of Piper sarmentosum organs approached by LC-MS-based metabolic profiling[J]. Natural Products and Bioprospecting, 2024, 14(4): 30-30.

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Comparative metabolite analysis of Piper sarmentosum organs approached by LC-MS-based metabolic profiling

Comparative metabolite analysis of Piper sarmentosum organs approached by LC-MS-based metabolic profiling

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