Alleviative Effects of Foliar TiO2-NPs Pretreatment on Wheat Seedling Growth under UV-B Stress

doi:10.7525/j.issn.1673-5102.2025.04.011

Abstract

Abstract:

The aim of this study was to reveal the alleviating effect of foliar pretreatment with TiO₂-NPs （titanium dioxide nanoparticles） on the growth of wheat seedlings under UV-B stress. Using T.aestivum as material， the effects of leaf TiO₂-NPs pretreatment on mitosis， DNA damage， antioxidant enzyme system， and antioxidant-related genes in the alleviation of UV-B stress were comprehensively studied at the cellular， physiological， biochemical， and genetic levels. The results showed that 10 mg⋅L^-1 TiO₂-NPs foliar pretreatment significantly alleviated the inhibition of wheat seedling growth under low-（5.04 kJ⋅m^-2） and high-dose（10.08 kJ⋅m^-2） UV-B stress conditions. After UV-B stress， plant height of TiO₂-NPs-treated wheat seedlings increased by 44.71% and 127.43%， root length increased by 38.92% and 159.10%， fresh weight increased by 50.03% and 78.67%， and dry weight increased by 37.33% and 126.84%， respectively. The proportion of mitotic abnormalities in root tip cells of TiO₂-NPs pre-treated wheat seedlings was only 39.57% and 28.63% under low and high doses of UV-B stress， respectively， and the CPDs content in cells decreased by 60.43% and 48.81%， respectively， compared with the control group. TiO₂-NPs foliar pretreatment increased the flavonol content by 1.44 times and 1.53 times under low- and high-dose UV-B stress， respectively， significantly increased the expression of flavonol synthesis genes（CHS and CHI） and SOD， and improved the ROS scavenging ability of wheat seedlings. In conclusion， foliar pretreatment with 10 mg⋅L^-1 TiO₂-NPs significantly improved the activity of plant antioxidant system， reduced the ROS-caused damage on wheat plants， and improved the tolerance of plants to UV-B stress.

Key words: Triticum aestivum, UV-B stress, TiO₂-NPs, antioxidant enzyme activity, stress resistance

CLC Number:

Jianhua WANG, Huize CHEN, Rong HAN. Alleviative Effects of Foliar TiO₂-NPs Pretreatment on Wheat Seedling Growth under UV-B Stress[J]. Bulletin of Botanical Research, 2025, 45(4): 592-602.

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References 32

[1]	WITZE A.Rare ozone hole opens over Arctic—and it’s big［J］.Nature，2020，580：18-19.
[2]	SHARMA A， SHARMA B， HAYES S，et al.UVR8 disrupts stabilisation of PIF5 by COP1 to inhibit plant stem elongation in sunlight［J］.Nature Communications，2019，10（1）：4417.
[3]	YANG Y， ZHANG L B， CHEN P，et al.UV-B photoreceptor UVR8 interacts with MYB73/MYB77 to regulate auxin responses and lateral root development［J］.The EMBO Journal，2020，39（2）：e101928.
[4]	LIANG T， MEI S L， SHI C，et al.UVR8 interacts with BES1 and BIM1 to regulate transcription and photomorphogenesis in Arabidopsis ［J］.Developmental Cell，2018，44（4）：512-523.e5.
[5]	WANG H J， LIU S H， WANG T L，et al.The moss flavone synthase I positively regulates the tolerance of plants to drought stress and UV-B radiation［J］.Plant Science，2020，298：110591.
[6]	韩榕，岳明，王勋陵.He-Ne激光对小麦幼苗增强UV-B辐射损伤的修复效应［J］.西北植物学报，2002，22（2）：263-269.
	HAN R， YUE M， WANG X L.The damage repair effects of He-Ne laser on wheat seedlings exposed to enhanced ultraviolet-B irradiation［J］.Acta Botanica Boreali-Occidentalia Sinica，2002，22（2）：263-269.
[7]	韩榕，王勋陵，岳明，等.增强UV-B辐射对小麦体细胞分裂的影响［J］.遗传学报，2002，29（6）：537-541.
	HAN R， WANG X L， YUE M，et al.Effects of the enhanced UV-B radiation on the body cell mitosis of the wheat［J］.Acta Genetica Sinica，2002，29（6）：537-541.
[8]	PRIESTER J H， MORITZ S C， ESPINOSA K，et al.Damage assessment for soybean cultivated in soil with either CeO₂ or ZnO manufactured nanomaterials［J］.Science of the Total Environment，2017，579：1756-1768.
[9]	LANDA P， VANKOVA R， ANDRLOVA J，et al.Nanoparticle-specific changes in Arabidopsis thaliana gene expression after exposure to ZnO，TiO₂，and fullerene soot［J］.Journal of Hazardous Materials，2012，241/242：55-62.
[10]	USMAN M， FAROOQ M， WAKEEL A，et al.Nanotechnology in agriculture：current status，challenges and future opportunities［J］.Science of the Total Environment，2020，721：137778.
[11]	TIAN L Y， SHEN J P， SUN G X，et al.Foliar application of SiO₂ nanoparticles alters soil metabolite profiles and microbial community composition in the pakchoi（Brassica chinensis L.） rhizosphere grown in contaminated mine soil［J］.Environmental Science & Technology，2020，54（20）：13137-13146.
[12]	KAH M， KOOKANA R S， GOGOS A，et al.A critical evaluation of nanopesticides and nanofertilizers against their conventional analogues［J］.Nature Nanotechnology，2018，13（8）：677-684.
[13]	MIKULA K， IZYDORCZYK G， SKRZYPCZAK D，et al.Controlled release micronutrient fertilizers for precision agriculture-a review［J］.Science of the Total Environment，2020，712：136365.
[14]	ULLAH S， ADEEL M， ZAIN M，et al.Physiological and biochemical response of wheat（Triticum aestivum） to TiO₂ nanoparticles in phosphorous amended soil：a full life cycle study［J］.Journal of Environmental Management，2020，263：110365.
[15]	RAO S， SHEKHAWAT G S.Phytotoxicity and oxidative stress perspective of two selected nanoparticles in Brassica juncea ［J］.3 Biotech，2016，6（2）：244.
[16]	BAKSHI M， LINÉ C， BEDOLLA D E，et al.Assessing the impacts of sewage sludge amendment containing nano-TiO₂ on tomato plants：a life cycle study［J］.Journal of Hazardous Materials，2019，369：191-198.
[17]	LIU J， WANG W X.The protective roles of TiO₂ nanoparticles against UV-B toxicity in Daphnia magna ［J］.Science of the Total Environment，2017，593/594：47-53.
[18]	DEHKOURDI E H， MOSAVI M.Effect of anatase nanoparticles（TiO₂） on parsley seed germination （Petroselinum crispum） in vitro［J］.Biological Trace Element Research，2013，155（2）：283-286.
[19]	MIDDEPOGU A， HOU J， GAO X，et al.Effect and mechanism of TiO₂ nanoparticles on the photosynthesis of Chlorella pyrenoidosa ［J］.Ecotoxicology and Environmental Safety，2018，161：497-506.
[20]	WANG G C， LUO Z K， HAN P F，et al.Critical carbon input to maintain current soil organic carbon stocks in global wheat systems［J］.Scientific Reports，2016，6：19327.
[21]	SHULL T E， KUREPA J， SMALLE J A.Anatase TiO₂ nanoparticles induce autophagy and chloroplast degradation in thale cress（Arabidopsis thaliana）［J］.Environmental Science & Technology，2019，53（16）：9522-9532.
[22]	CHEN H Z， HAN R.Characterization of actin filament dynamics during mitosis in wheat protoplasts under UV-B radiation［J］.Scientific Reports，2016，6：20115.
[23]	HEIKAL Y M， ŞUŢAN N A， RIZWAN M，et al.Green synthesized silver nanoparticles induced cytogenotoxic and genotoxic changes in Allium cepa L. varies with nanoparticles doses and duration of exposure［J］.Chemosphere，2020，243：125430.
[24]	SUN C， ZHAO C， WANG G H，et al.Cerium oxide nanoparticles alleviate enhanced UV-B radiation-induced stress in wheat seedling roots by regulating reactive oxygen species［J］.Phyton-International Journal of Experimental Botany，2025，94（2）：455-479.
[25]	COX A， VENKATACHALAM P， SAHI S，et al.Silver and titanium dioxide nanoparticle toxicity in plants：a review of current research［J］.Plant Physiology and Biochemistry，2016，107：147-163.
[26]	SINGH J， LEE B K.Influence of nano-TiO₂ particles on the bioaccumulation of Cd in soybean plants（Glycine max）：a possible mechanism for the removal of Cd from the contaminated soil［J］.Journal of Environmental Management，2016，170：88-96.
[27]	ABDEL LATEF A A H， SRIVASTAVA A K， EL-SADEK M S A，et al.Titanium dioxide nanoparticles improve growth and enhance tolerance of broad bean plants under saline soil conditions［J］.Land Degradation & Development，2018，29（4）：1065-1073.
[28]	SUN C， ZHAO C， WANG G H，et al.Cerium oxide nanoparticles ameliorate Arabidopsis thaliana root damage under UV-B stress by modulating the cell cycle and auxin pathways［J］.Protoplasma，2025，262（4）：895-913.
[29]	ZUO R Z， LIU H G， XI Y，et al.Nano-SiO₂ combined with a surfactant enhanced phenanthrene phytoremediation by Erigeron annuus（L.） Pers［J］.Environmental Science and Pollution Research，2020，27（16）：20538-20544.
[30]	SENDRA M， YESTE P M， MORENO-GARRIDO I，et al.CeO₂ NPs，toxic or protective to phytoplankton？ Charge of nanoparticles and cell wall as factors which cause changes in cell complexity［J］.Science of the Total Environment，2017，590/591：304-315.
[31]	VANHAELEWYN L， PRINSEN E， VAN DER STRA-ETEN D，et al.Hormone-controlled UV-B responses in plants［J］.Journal of Experimental Botany，2016，67（15）：4469-4482.
[32]	MORADI RIKABAD M， POURAKBAR L， SIAVASH MOGHADDAM S，et al.Agrobiological，chemical and antioxidant properties of saffron（Crocus sativus L.） exposed to TiO₂ nanoparticles and ultraviolet-B stress［J］.Industrial Crops and Products，2019，137：137-143.

基因 Gene	正向引物 Forward primer	反向引物 Reverse primer
查尔酮合成酶 CHS	AAGATCACCAAGAGCGACCACAT	AGGATCTCCTCHGTNAGGTGC
查尔酮异构酶 CHI	CGGCGAGTTCGAGAAGTTCAC	TTCCAGTAGGCGACGCAGTT
超氧化物歧化酶 SOD	CAGGACCCTCTTGTGACCAAAG	CCAGTTCACCACCTTCCAGATG
过氧化物酶 POD	ACTTCCACGACTGCTTTGTCCA	CATATACTCTCCAGCTGGGTCTTG
肌动蛋白 ACTIN	GCCAACAGGGAGAAGATGACA	CATAGATTGGGACTGTGTGACTGAC

处理组 Treatment	细胞总数 Total number of cells observed	分裂期细胞总数 Total number of dividing cells	异常分裂细胞总数 Total number of aberration cells	异常分裂比例 Aberration percentage/%
T	2 730	121	0	0^e
C	3 215	147	0	0^e
LT	2 964	134	7	5.31±1.23^d
L	2 430	128	16	13.42±2.35^b
HT	2 624	137	14	9.84±1.62^c
H	2 507	118	27	34.37±5.28^a

Alleviative Effects of Foliar TiO₂-NPs Pretreatment on Wheat Seedling Growth under UV-B Stress

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