Response of Arabidopsis thaliana Histone Chaperone HIRA to Enhanced UV-B Radiation

doi:10.7525/j.issn.1673-5102.2025.04.010

Abstract

Abstract:

UV-B radiation significantly inhibits plant growth， leading to short roots and DNA damage. Research has shown that the histone chaperone HIRA（HISTONE REGULATOR A） plays a role in chromatin-related DNA damage repair. In this study， using the Columbia ecotype of Arabidopsis thaliana and the hira mutant as materials， the comparison of phenotypic and physiological indicators between the control group （-UV-B） and the UV-B treatment group（+UV-B） revealed that the absence of the HIRA gene led to shorter roots and smaller plants in A. thaliana. After UV-B treatment， the roots of hira mutant were significantly shorter than those of the wild type， with increased levels of reactive oxygen species and malondialdehyde， and decreased levels of soluble protein and chlorophyll. Subcellular localization of A. thaliana transformation showed that HIRA was located in the nucleus. UV-B radiation caused chromosome bridges and abnormal mitosis in the root tip meristematic zone cells of the mutant. Further research results indicated that UV-B radiation significantly upregulated the expression of DNA damage repair related genes. In conclusion， HIRA was involved in the process of cell mitosis and damage repair under UV-B radiation.

Key words: histone chaperone HIRA, UV-B, abnormal mitosis, DNA damage repair

CLC Number:

Qianwen MAO, Guohua WANG, Rong HAN. Response of Arabidopsis thaliana Histone Chaperone HIRA to Enhanced UV-B Radiation[J]. Bulletin of Botanical Research, 2025, 45(4): 580-591.

Figures/Tables 11

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