Stumping Effect on Rejuvenation of Twigs and Rooting of Softwood Cuttings of Acer Mandshuricum

doi:10.7525/j.issn.1673-5102.2025.02.009

Abstract

Abstract:

Acer mandshuricum is a colourful leafy tree species with great ornamental value in Northeast China， but it is difficult to reproduce. The aim of this study was to improve the rooting rate of A. mandshuricum softwood cuttings and to clarify the relationship between stumping treatment and rooting of softwood cuttings. In 2023， the cross-sectional anatomical structure and growth index of twigs， the physiological and biochemical index of leaves， and the rooting index of softwood cuttings of Pc-2（stumped in 2022）， Pc-1（stumped in 2023） were observed， using the twigs of A. mandshuricum without stumping（Pc-CK） as a control. The results showed that： the rooting rate， average root length， average number of roots， and root effect index of Pc-1 softwood cuttings were higher than those of Pc-2（P<0.05） and Pc-CK（P<0.05）. The thickness of the epidermal and cortical collenchyma and the thickness of the xylem in the cross-section of their twigs from small to large were Pc-1， Pc-2 and Pc-CK（P<0.05）. The length， basal diameter， leaf area， leaf fresh weight， and water content of Pc-1 twigs were significantly higher than those of Pc-CK（P<0.05）. The net photosynthetic rate and stomatal conductance of Pc-1 leaves were significantly increased by 90% and 89%（P<0.05） respectively， compared to Pc-CK. The contents of chlorophyll a， carotenoid， soluble sugar， and soluble protein， as well as the activities of superoxide dismutase（SOD）， peroxidase（POD）， and catalase（CAT） in Pc-1 leaves were significantly higher than those of Pc-CK（P<0.05）. The contents of malondialdehyde（MDA）， total flavonoids， and total phenolics in Pc-CK leaves were significantly higher than those in Pc-1（P<0.05）. In conclusion， the stumping treatment made the twigs of A. mandshuricum rejuvenated， which promoted the rooting from softwood cuttings. The Pc-1 twigs showed better juvenile characteristics and rooting effect on softwood cuttings， which provided support for the softwood cutting propagation of A. mandshuricum.

Key words: Acer mandshuricum, stumping, microstructure, photosynthetic characteristics, antioxidant enzyme activity

CLC Number:

S792.35

Linping TIAN, Wenzhe HAN, Jun WU, Yuandong MA, Yu QI, Zhaoning WANG, Chunming LI, Haixia LI, Huanzhen LIU, Haijuan FAN. Stumping Effect on Rejuvenation of Twigs and Rooting of Softwood Cuttings of Acer Mandshuricum[J]. Bulletin of Botanical Research, 2025, 45(2): 228-240.

Figures/Tables 9

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