Photosynthetic Characteristics of Sassafras tzumu under Cadmium Stress in Soil

doi:10.7525/j.issn.1673-5102.2021.04.005

Abstract

Abstract:

To explore the photosynthetic characteristics of Sassafras tzumu Hemsl. under Cd stress ， the potted method was used. With 5 cadmium concentration levels（CK：0 mg·kg^-1， T1：5 mg·kg^-1， T2：20 mg·kg^-1， T3：50 mg·kg^-1， T4：100 mg·kg^-1） were set， Chlorophyll content， photosynthetic gas exchange parameters and photosynthetic-light response curve of S. tzumu leaves matching each treatment were measured respectively. The results showed that：①As the concentration of Cd increased， chlorophyll a， chlorophyll b and the total amount of chlorophyll in S. tzumu leaves increased first and then decreased， the maximum was in T2. ②With the Cd concentration increased， the net photosynthetic rate（P_n） and stomatal conductance（G_s） of leaves showed a downward trend. There were no significant differences in the intercellular carbon dioxide concentration（C_i） and transpiration rate（T_r） between treatments. The photosynthetic rate of S. tzumu leaves was mainly limited by non-stomatal factors. ③With the Cd concentration increased， P_n_max（maximum net photosynthetic rate） of S. tzumu leaves decreased significantly. Under T1 processing conditions， the initial quantum efficiency（α） of S. tzumu leaves got maximum value； The dark respiration rate（R_d）， light compensation point（LCP） and light saturation point（LSP） of S. tzumu leaves decreased respectively. In conclusion， under the stress of different concentrations of Cd， the chlorophyll content of S. tzumu first increased and then decreased， which was higher than that of CK at T1 and T2， reflecting the “low promoting and high suppressing” effect of Cd stress. With the Cd concentration increased， photosynthetic gas exchange parameters and photosynthetic-light response parameters showed a downward trend compared with CK， indicating that Cd stress had an obvious inhibitory effect on S. tzumu photosynthesis.

Key words: Sassafras tzumu, Cadmium stresses, photosynthetic characteristics, gas exchange parameter

CLC Number:

S792.22

Hong-Yi ZHAO, Jue-Lan GUAN, Xue-Yuan ZHANG, Qing LIANG, Jian ZHANG, Hong-Ling HU. Photosynthetic Characteristics of Sassafras tzumu under Cadmium Stress in Soil[J]. Bulletin of Botanical Research, 2021, 41(4): 506-513.

References

1	孙婕妤，刘艳秋，李佰林，等.植物对镉的耐性机制以及对镉污染土壤修复的研究进展［J］.江苏农业科学，2018，46（7）：12-19．
1	Sun J Y，Liu Y Q，Li B L，et al.Research progress on mechanism of plant tolerance to cadmium and remediation of cadmium contaminated soil［J］.Jiangsu Agricultural Sciences，2018，46（7）：12-19.
2	曾燕蓉，朱方容，林强，等.镉胁迫对水培桑苗生长的影响［J］.南方农业学报，2019，50（2）：247-256．
2	Zeng Y R，Zhu F R，Lin Q，et al.Impacts of cadmium stress on growth of hydroponic mulberry seedlings［J］.Journal of Southern Agriculture，2019，50（2）：247-256.
3	Dalcorso G，Farinati S，Maistri S，et al.How plants cope with cadmium：staking all on metabolism and gene expression［J］.Journal of Integrative Plant Biology，2008，50（10）：1268-1280.
4	郑元，赵忠，周慧，等.晴天和阴天对刺槐光合生理特性的影响［J］.林业科学，2011，47（5）：60-67.
4	Zheng Y，Zhao Z，Zhou H，et al.Effects of sunny and cloudy days on photosynthetic and physiological characteristics of black locust［J］.Scientia Silvae Sinicae，2011，47（5）：60-67.
5	李宏林，徐当会，杜国祯.青藏高原高寒沼泽湿地在退化梯度上植物群落组成的改变对湿地水分状况的影响［J］.植物生态学报，2012，36（5）：403-410.
5	Li H L，Xu D H，Du G Z.Effect of change of plant community composition along degradation gradients on water conditions in an alpine swamp wetland on the Qinghai-Tibetan Plateau of China［J］.Chinese Journal of Plant Ecology，2012，36（5）：403-410.
6	Dean C，Pichersky E，Dunsmuir P.Structure，evolution，and regulation of rbcS genes in higher plants［J］.Annual Review of Plant Physiology and Plant Molecular Biology，1989，40：415-439.
7	臧畅，吕志强，董莲春，等.不同性别桑树幼苗对镉与酸雨复合处理的生长响应及镉积累差异［J］.应用生态学报，2018，29（3）：969-975.
7	Zang C，Lyu Z Q，Dong L C，et al.Variations in growth response and cadmium accumulation of different gender Morus alba seedlings to combined treatments of Cd and acid rain［J］.Chinese Journal of Applied Ecology，2018，29（3）：969-975.
8	Vecchia F D，Rocca N L，Moro I，et al.Morphogenetic，ultrastructural and physiological damages suffered by submerged leaves of Elodea canadensis exposed to cadmium［J］.Plant Science，2005，168（2）：329-338.
9	刘会超，刘孟刚，姚连芳，等.镉胁迫对银条生物量及光合特性的影响［J］.华北农学报，2010，25（4）：162-165.
9	Liu H C，Liu M G，Yao L F，et al.Effects of cadmium stress on biomass and photosynthetic characteristics of Stachys floridana［J］.Acta Agriculturae Boreali-Sinica，2010，25（4）：162-165.
10	罗紫东，关华德，章新平，等.枫香叶片衰老过程中光合能力的变化［J］.应用生态学报，2016，27（10）：3129-3136.
10	Luo Z D，Guan H D，Zhang X P，et al.Changes in photosynthetic capacity during leaf senescence of Liquidambarformosana［J］.Chinese Journal of Applied Ecology，2016，27（10）：3129-3136.
11	王海珍，韩路，徐雅丽，等.干旱胁迫下胡杨光合光响应过程模拟与模型比较［J］.生态学报，2017，37（7）：2315-2324.
11	Wang H Z，Han L，Xu Y L，et al.Simulated photosynthetic responses of Populus euphratica during drought stress using light-response models［J］.Acta Ecologica Sinica，2017，37（7）：2315-2324.
12	乐佳兴，田秋玲，吴焦焦，等.无患子幼苗的生长和光合特性对重庆低山丘陵区不同生境的响应［J］.北京林业大学学报，2019，41（6）：75-85.
12	Le J X，Tian Q L，Wu J J，et al.Response of seedling growth and photosynthetic characteristics of Sapindusmukorossi to different habitats in low mountainous upland region of Chongqing，southwestern China［J］.Journal of Beijing Forestry University，2019，41（6）：75-85.
13	伍平澜，罗祖筠.贵州珍贵树种檫木［J］.贵州林业科技，1981（3）：8-12.
13	Wu P L，Luo Z Y.Precious sassafras of Guizhou［J］.Guizhou Forestry Science and Technology，1981（3）：8-12.
14	中国科学院中国植物志编辑委员会.中国植物志［M］.北京：科学出版社，1982.
14	Editorial Committee of flora of China，Chinese Academy of Sciences.Flora of China［M］.Beijing：Science Press，1982.
15	陈喜友.檫木不同混交方式生长情况研究［J］.安徽林业科技，2015，41（4）：9-11.
15	Chen X Y.Study on the growth of Sassafras tzumu in plantations of different mixed patterns［J］.Anhui Forestry Science and Technology，2015，41（4）：9-11.
16	程勇，吴际友，唐春.檫木种子贮藏试验［J］.湖南林业科技，2014，41（2）：28-30，34.
16	Cheng Y，Wu J Y，Tang C.The storage of seed of Sassafras tzumu［J］.Hunan Forestry Science and Technology，2014，41（2）：28-30，34.
17	沈燕，田大伦，项文化，等.天然次生檫木枫香混交林生物量及生产力研究［J］.中南林业科技大学学报，2011，31（5）：26-30.
17	Shen Y，Tian D L，Xiang W H，et al.Biomass and productivity of natural secondary Sassafras tzumu and Liquidambar formosana mixed forest［J］.Journal of Central South University of Forestry & Technology，2011，31（5）：26-30.
18	Küpper H，Küpper F，Spiller M.Environmental relevance of heavy metal-substituted chlorophylls using the example of water plants［J］.Journal of Experimental Botany，1996，47（2）：259-266.
19	Kalaji H M，Loboda T.Photosystem II of Barley seedlings under cadmium and lead stress［J］.Plant，Soil and Environment，2007，53（12）：511-516.
20	Chatzistathis T A，Papadakis I E，Therios I N，et al.Is chlorophyll fluorescence technique a useful tool to assess manganese deficiency and toxicity stress in olive plants？［J］.Journal of Plant Nutrition，2010，34（1）：98-114.
21	孙小玲，许岳飞，马鲁沂，等.植株叶片的光合色素构成对遮阴的响应［J］.植物生态学报，2010，34（8）：989-999.
21	Sun X L，Xu Y F，Ma L Y，et al.A review of acclimation of photosynthetic pigment composition in plant leaves to shade environment［J］.Chinese Journal of Plant Ecology，2010，34（8）：989-999.
22	陈霞霞，蒲高忠，黄玉清，等.铊和镉胁迫对芦竹生长及光合特征的影响［J］.广西植物，2019，39（6））：743－751.
22	Chen X X，Pu G Z，Huang Y Q，et al.Effects of thallium and cadmium stress on growth and photosynthetic characteristics of Arundo donax［J］.Guihaia，2019，39（6）：743-751.
23	秦天才，阮捷，王腊娇.镉对植物光合作用的影响［J］.环境科学与技术，2000（S1）：33-35，44．
23	Qin T C，Ruan J，Wang L J.Effect of Cd on the plant photosynthesis［J］.Environmental Science & Technology，2000（S1）：33-35，44.
24	鲁艳，李新荣，何明珠，等.重金属对盐生草光合生理生长特性的影响［J］.西北植物学报，2011，31（2）：370-376．
24	Lu Y，Li X R，He M Z，et al.Photosynthesis and physiological characteristics in Halogeton glomeratus with heavy metal treatments［J］.Acta Botanica Boreali-occidentalia Sinica，2011，31（2）：370-376.
25	Farquhar G D，Sharkey T D.Stomatal conductance and photosynthesis［J］.Annual Review of Plant Physiology，1982，33：317-345.
26	叶子飘.光合作用对光和CO₂响应模型的研究进展［J］.植物生态学报，2010，34（6）：727-740.
26	Ye Z P.A review on modeling of responses of photosynthesis to light and CO₂［J］.Chinese Journal of Plant Ecology，2010，34（6）：727-740.
27	王海珍，韩路，徐雅丽，等.胡杨异形叶叶绿素荧光特性对高温的响应［J］.生态学报，2011，31（9）：2444-2453.
27	Wang H Z，Han L，Xu Y L，et al.Response of chlorophyll fluorescence characteristics of Populus euphratica heteromorphic Leaves to high temperature［J］.Acta Ecologica Sinica，2011，31（9）：2444-2453.
28	李理渊，李俊，同小娟，等.不同光环境下栓皮栎和刺槐叶片光合光响应模拟［J］.应用生态学报，2018，29（7）：2295-2306.
28	Li L Y，Li J，Tong X J，et al.Simulation on photosynthetic light-responses of leaves of Quercusvariabilis and Robiniapseudoacacia under different light conditions［J］.Chinese Journal of Applied Ecology，2016，29（7）：2295-2306.
29	魏明月，云菲，刘国顺，等.不同光环境下烟草光合特性及同化产物的积累与分配机制［J］.应用生态学报，2017，28（1）：159-168.
29	Wei M Y，Yun F，Liu G S，et al.Response of photosynthetic characteristics and accumulation and distribution of assimilation products in tobacco to different light environments［J］.Chinese Journal of Applied Ecology，2017，28（1）：159-168.
30	李明月，王健，王振兴，等.模拟氮沉降条件下木荷幼苗光合特性、生物量与C、N、P分配格局［J］.生态学报，2013，33（5）：1569-1577.
30	Li M Y，Wang J，Wang Z X，et al.Photosynthetic characteristics，biomass allocation，C，N and P distribution of Schima superba seedlings in response to simulated nitrogen deposition［J］.Acta Ecologica Sinica，2013，33（5）：1569-1577.
31	王小菲，高文强，刘建锋，等.不同生境对栓皮栎幼苗光合生理特性的影响［J］.生态学报，2016，36（24）：8062-8070.
31	Wang X F，Gao W Q，Liu J F，et al.Effects of habitat change on the photosynthetic characteristics of Quercus variabilis seedlings［J］.Acta Ecologica Sinica，2016，36（24）：8062-8070.
32	王富刚，徐伟洲，亢福仁，等.不同坡向条件下小叶杨光合光响应曲线研究［J］.水土保持研究，2015，22（6）：177-182.
32	Wang F G，Xu W Z，Kang F R，et al.Photosynthetic light-response curves of Populussimonii under different slope aspects［J］.Research of Soil and Water Conservation，2015，22（6）：177-182.
33	齐欣，曹坤芳，冯玉龙.热带雨林蒲桃属3个树种的幼苗光合作用对生长光强的适应［J］.植物生态学报，2004，28（1）：31-38.
33	Qi X，Cao K F，Feng Y L.Photosynthetic acclimation to different growth light environments in seedlings of three tropical rainforest Syzygium species［J］.Acta Phytoecologica Sinica，2004，28（1）：31-38.

[1]	Tianmeng REN, Yu BAO, Yaxun SU, Qingbin CHEN, Liying WEI, Ruoxuan JI, Xiao YU, Xinli XIA, Weilun YIN, Chao LIU. Effects of Different Pruning Treatments on Growth and Photosynthetic Characteristics of 10-year-old Triploid Populus tomentosa [J]. Bulletin of Botanical Research, 2023, 43(6): 846-856.
[2]	Chunyan WEI, Yueling LI, Zexin JIN, Guangyu LUO, Chao CHEN, Fangquan SHAN. Effects of Shading on Photosynthetic Characteristics and Non-structural Carbohydrate Content of Heptacodium miconioides Seedlings [J]. Bulletin of Botanical Research, 2022, 42(6): 1096-1105.
[3]	WU Jian-Hui, FAN Wei-Fang, NIU Zhe, ZHANG Jing, GAO Peng-Fei, LI Wen. Effects of Exogenous Methyl Jasmonate on Photosynthesis and Physiological Characteristics of Scutellaria regeliana under Drought Stress [J]. Bulletin of Botanical Research, 2020, 40(3): 360-367.
[4]	QIAO Bin-Jie, WANG De-Qiu, GAO Hai-Yan, LI Zhao-Min, GE Li-Li, DING Wen-Ya, ZHAO Xi-Yang. Photosynthetic and Stomatal Morphological Variation of Poplar Clones in Seedling Stage under Drought Stress [J]. Bulletin of Botanical Research, 2020, 40(2): 177-188.
[5]	HU Ju-Wei, DAI Xin, SONG Tao, SUN Guang-Yu. Effects of Different Light Qualities on Growth and Photosynthetic Characteristics of Mulberry Seedlings [J]. Bulletin of Botanical Research, 2019, 39(4): 481-489.
[6]	WAN Xue-Bing, WANG Qing-Gui, YAN Guo-Yong, XING Ya-Juan. Response of Ecological Stoichiometric Characteristics and Photosynthetic Characteristics of Plant Leaves to Long-term N Deposition in Natural Secondary Forest [J]. Bulletin of Botanical Research, 2019, 39(3): 407-420.
[7]	XU Hai-Jun, CHENG Xin-Yu, WANG Xiao-Fei, GUAN Xiang-Jun. Effects of Different Nitrogen Level on Growths of Moringa oleifera Seedling under Northern Facilities of China [J]. Bulletin of Botanical Research, 2019, 39(1): 54-60.
[8]	YIN Dong-Sheng, SHEN Hai-Long, WEI Xiao-Hui. Effects of Shading on Physocarpus amurensis Seedlings Photosynthetic Ability and Carbohydrate Accumulation [J]. Bulletin of Botanical Research, 2017, 37(6): 841-847.
[9]	SHU Wen-Jiang, CHAI Sheng-Feng, LI Zhao-Hai, FAN Jin-Shun, TANG Jian-Min, ZHU Shu-Fang, HE Zhi-Hong. Effects of Different Light Intensities on the Growth and Photosynthesis of Symplocos tetragona Seedlings [J]. Bulletin of Botanical Research, 2017, 37(4): 556-562.
[10]	LI Xin-Yan, TENG Zhi-Yuan, XU Qi-Jiang, ZHANG Hui-Hui, ZHENG Bao-Jiang. Effects of Shading on Anatomical Structure and Photosynthetic Characteristics of Ribes nigrum L. Leaves [J]. Bulletin of Botanical Research, 2017, 37(4): 521-528.
[11]	YAN Xiao-Hong, ZHOU Bing, HU Wen-Hai, WANG Ning, YIN Zeng-Fang. Effects of Drought Stress on Photosynthetic Characteristics in Leaves of Invasive Bidens frondosa and its Native Associated Lactuca indica [J]. Bulletin of Botanical Research, 2016, 36(6): 931-941.
[12]	ZHANG Zhong-Hua1,2；TANG Zhong-Hua1；YANG Feng-Jian1*；WANG Hua3；ZHANG Jun1；ZU Yuan-Gang1. Photosynthetic Characteristics and Its Micro-environmental Limiting Factors of Two Main Oil Peony [J]. Bulletin of Botanical Research, 2014, 34(6): 770-775.
[13]	HAN Yu;;LIU Shi-Shan;;LIANG Yan-Li;;ZHAO Qing-Yun;LI Jian;XU Wen-Guo;YAN Dui;WU Shi-Bin;YAN Suo;LIU Chun;XIE Shi-Qing;CHEN Jun-Wen;. Photosynthesis and Photorotection in Amorphophallus konjac* and Amorphophallus xiei Grown at a Light Gradient [J]. Bulletin of Botanical Research, 2013, 33(6): 676-683.
[14]	HE An-Na;;OU Li-Jun;;LI Sheng-Hua;;TAN Xiao-Li. Correlation Analysis between Photosynthetic Characteristics and Contents of Active Ingredients in Saxifraga stolonifera Curt. under Different Light and Temperature Conditions [J]. Bulletin of Botanical Research, 2013, 33(5): 587-592.
[15]	HE An-Na;;LIN Wen-Qiang;YAO Yi. Effect of Shading on the Photosynthetic Characteristics of Saxifrage stolonifera Curt. [J]. Bulletin of Botanical Research, 2012, 32(6): 657-661.