An Optimized HPLC Procedure for Analyzing Three Endogenous Hormones in Tulip Bulbs

doi:10.7525/j.issn.1673-5102.2021.01.016

Abstract

Abstract:

Agilent HPLC was used to establish a method for simultaneous determination of endogenous hormones GA₃， IAA and ABA in tulip bulbs， and an extraction method of isopropanol was established. The external standard method was used for determination， C₁₈ reverse phase column， mobile phase A（methanol）∶B（phosphate buffer pH=3.5）=45∶55； flow rate 1 mL·min^-1； detection wavelength 0-3.2 min 265nm， 3.0-4.5 min 212 nm， 4.5-6.5 min 218 nm； 6.5-13.0 min 265 nm； column temperature 20℃ for HPLC determination. Isopropyl alcohol extractant and dichloromethane low temperature shaker to purify the endogenous hormones in the bulb. The whole process was simple to operate， which took only 2.0-2.5 h to complete the hormone purification. The linear correlation of the detection methods was above 0.995. Detection limits of GA₃， IAA and ABA were 200 ng·mL^-1， 5 ng·mL^-1 and 20 ng·mL^-1， respectively. Recovery rate of Purification method was 84.812%-95.679%. The relative standard deviation was 6.432%-2.831%. A stable baseline and high accuracy， and a satisfactory peak shape， simple operation， less loss of hormones can be obtained in each link by extracting the endogenous hormone.

Key words: HPLC, tulip bulb, endogenous hormones

CLC Number:

S682.2+63

Yang-Jing ZHAO, Nan TANG, Dao-Cheng TANG, Jing ZHANG. An Optimized HPLC Procedure for Analyzing Three Endogenous Hormones in Tulip Bulbs[J]. Bulletin of Botanical Research, 2021, 41(1): 130-137.

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