Simultaneous Determination of Baschnaloside and Rossicaside B in Boschniakia rossica by HPLC

doi:10.7525/j.issn.1673-5102.2021.03.014

Abstract

Abstract:

In order to establish an HPLC method for the simultaneous determination of baschnaloside and rossicaside B in Boschniakia rossica dry whole， a chromatographic column： Klimail 100-5 C₁₈ column（250 mm×4.6 mm， 5 μm）； mobile phase： acetonitrile as mobile phase A， 0.5% formic acid aqueous solution as mobile phase B， gradient elution conditions： 0?12 min， 15% A； 12?30 min， 15%?20% A； 30?40 min， 20%?25% A； 40?45 min， 25%?30% A； 45?60 min， 30%?100% A； flow rate： 1.0 mL·min^-1； detection wavelength： 260 nm. Column temperature： 30℃. Injection volume： 20 μL. The linear range of naringin of baschnaloside was 4.688?150 μg·g^-1（R²=0.999 5）； the linear range of rossicaside B was 3.438?110 μg·g^-1（R²=0.999 1）； the average recoveries were 97.86% and 96.55%， and RSDs were 1.01 and 1.23（n=9）， respectively. A high-performance liquid chromatography method was developed for the simultaneous determination of two components， baschnaloside and rossicaside B in B.rossica. The methodological verification results show that the method is simple， fast， and reproducible， and can be used to determine the contents of baschnaloside and rossicaside B in B.rossica.

Key words: Boschniakia rossica, HPLC, baschnaloside, rossicaside B

CLC Number:

S567.9

Min CHEN, Meng-Fei TIAN, Chang-Yin FENG, Xian-Ming MENG, Meng LUO, Fan-Song MU. Simultaneous Determination of Baschnaloside and Rossicaside B in Boschniakia rossica by HPLC[J]. Bulletin of Botanical Research, 2021, 41(3): 436-440.

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