Determination and Analysis of Alkaloid Content in Aconitum kusnezoffii Seeds from Different Areas

doi:10.7525/j.issn.1673-5102.2020.05.016

Abstract

Abstract: The experiment was conducted to determine the content of alkaloid in the seed of Aconitum kusnezoffii Reichb. from different habitats, and to compare its alkaloid content with its content in root and leaf. High pressure liquid chromatography(HPLC) was used to determine alkaloid content in seed of wild A.kusnezoffii collected from Zhalute of Tongliao City, Aru Kerqin of Chifeng City, and Bahrain of Chifeng City. The HPLC column used was Supersil ODS2 5 μm(250 mm×4.6 mm, 5 μm). The HPLC mobile phase was 0.1 mol·L^-1 methanol and triethylamine(V:V 75:25) and the flow rate was 0.8 mL·min^-1 under the detection wavelength of 235 nm with column temperature as 35℃. Two types of alkaloid were detected in the seed of A.kusnezoffii and the content of benzoyl aconitine was the highest while the content of diester alkaloid was comparatively lower. Total alkaloid content in seed content from Zhalute of Tongliao City, Alu Kerqin of Chifeng City, and Balin of Chifeng City was 7.647 9, 8.256 3 and 7.512 6 mg·g^-1, respectively. The total A.kusnezoffii seed alkaloid content collected from Tongliao was significantly higher than that from Chifeng City. The content of monoester alkaloid in A.kusnezoffii seed was significantly higher than diester alkaloid, and can be used for the comprehensive utilization, screening, and breeding of A.kusnezoffii germplasm with different seed alkaloid content.

Key words: different regions, Aconitum kusnezoffii seed, HPLC, alkaloid, benzoyl neoaconitine

CLC Number:

Q946.88

SHAN Dan, QU Lu-Lu, LI Xiao-Jie, MA Yi-Ming, WANG Jun-Jie. Determination and Analysis of Alkaloid Content in Aconitum kusnezoffii Seeds from Different Areas[J]. Bulletin of Botanical Research, 2020, 40(5): 768-774.

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