Genetic Diversity Analysis from Different Geographic Populations of Linaria vulgaris var. sinensis by rpl32-trnL Fragment

doi:10.7525/j.issn.1673-5102.2022.05.009

Abstract

Abstract:

In order to investigate the population genetic characteristics of Linaria vulgaris， four different geographic populations containing 62 individuals were analyzed by using rpl32-trnL fragment of chloroplast DNA. The results showed that there were 15 haplotypes and 76 loci existing in 4 populations. The total genetic diversity of L. vulgaris reached a high level（H_d=0.878， π=0.003 88， K=2.994）. The AMOVA analysis showed that the genetic variations were mainly maintained within the populations（51.49%）， which was consistent with the genetic characteristics of self-incompatibility in L. vulgaris. The genetic differentiation among four geographic populations was significant（F_ST=0.466 14）， while the gene flow between different geographic populations was rather low（N_m=0.29）. The genetic differentiation between different populations was positively correlated with the geographical distance but no significance（R²=0.36， P>0.05）. The results of neutrality test showed that the Heshui population（HS） might experience some bottleneck events during its evolution （Fu and Li’s D=-2.450 49，P<0.05）， and the evolutionary feature of the other populations was consistent with the neutral hypothesis. This study revealed that the reproductive strategies， geographic isolation， and habitat disturbance were not only responsible for the rather high level of genetic diversity in the populations distributed in the northern area of China as well as their genetic structure characteristics， but also it might provide a scientific basis for the further study of conservation genetics in L. vulgaris.

Key words: Linaria vulgaris, geographic populations, genetic diversity, rpl32-trnL

CLC Number:

Q949.777.8

Yujie ZHANG, Pengxia SHI, Fan ZHAO, Xiaodan CHEN, Kun SUN, Hui ZHANG. Genetic Diversity Analysis from Different Geographic Populations of Linaria vulgaris var. sinensis by rpl32-trnL Fragment[J]. Bulletin of Botanical Research, 2022, 42(5): 780-789.

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