Genetic Diversity of Critically Endangered Plants Petrocalamus luodianensis from Different Natural Populations

doi:10.7525/j.issn.1673-5102.2019.05.013

Abstract

Abstract: To reveal the endangered reasons of Petrocalamus luodianensis, and provide theoretical support for the protection and utilization of this species, and then form the best protecting strategy for the critically endangered species, we explored the genetic diversity of different natural populations of P.luodianensis and the correlation with environmental factors at the molecular level. The genetic diversity and heritable variation of sixteen natural populations were studied by POPGENE 32 and the technology marked by random amplified polymorphic DNA(RAPD). The results showed that 105 high reproducible, stable and discernible DNA fragments weight from 200-3 000 bp were generated from 8 RAPD primers and 98 polymorphic locis are included, and the PPL is 93.33%. At the species level, the effective number of alleles(N_e) is 1.494 2, the Nei's gene diversity(H) is 0.300 5, and the Shannon's information index(I) is 0.458 6. The Luowan(ZY1) population possessed the highest level of genetic diversity(PPL=60.95%, H=0.232 9 and I=0.345 1), while the Taopo(PT1) population showed the lowest level of genetic diversity(PPL=44.76%, H=0.170 0 and I=0.252 3). In the 16 natural populations, the coefficient of genetic differentiation(G_st) is 0.323 1, the gene flows(N_m) is 1.0478 and the(H_SP-H_POP)/H_SP based on Shannon's Information Index(I) is 0.342 9, a little higher than G_st. Therefore, there is a significant variation and abundant diversity of the P.luodianensis, the intra-population variation is greater than the inter-population, and the genetic variation exists mainly within the intra-population.

Key words: critically endangered plants, natural populations, genetic diversity, RAPD, gene

CLC Number:

S795.9

GUAN Rui-Ting, LIU Ji-Ming, WANG Min, CHEN Jing-Zhong, WU Meng-Yao, TONG Bing-Li. Genetic Diversity of Critically Endangered Plants Petrocalamus luodianensis from Different Natural Populations[J]. Bulletin of Botanical Research, 2019, 39(5): 740-751.

References

1.王正平,马乃训,张文燕.我国西部竹类一新属[J].竹子研究汇刊,2013,32(3):1-4.Wang Z P,Ma N X,Zhang W Y.A new genus of bamboos from west China[J].Journal of Bamboo Research,2013,32(3):1-4.
2.王正平,马乃训,张文燕.关于岩竹属和镰序竹属、悬竹属的讨论[J].竹子学报,2017,36(1):1-4.Wang Z P,Ma N X,Zhang W Y.The taxonomic distinctions between Drepanostachyum Keng f.,Ampelocalamus S.L.Chen,T.H.Wen et G.Y.Sheng and Petrocalamus Z.P.Wang,N.X.Ma et W.Y.Zhang[J].Journal of Bamboo Research,2017,36(1):1-4.
3.金祺,刘济明.不同生境条件对小蓬竹生长的影响[J].中南林业调查规划,2014,33(4):52-56,66.Jin Q,Liu J M.Study on the influence of different habitats for the growth of Drepanostachyum luodianense[J].Central South Forest Inventory and Planning,2014,33(4):52-56,66.
4.刘超,刘济明,蒙朝阳,等.竹类在水土保持中的作用——以小蓬竹为例[J].现代农业科技,2009,(14):276-278.Liu C,Liu J M,Meng C Y,et al.The role of bamboo in water and soil conservation-eg.for Drepanostachyum luodianense(Yi et R.S.Wang)Keng f.[J].Modern Agricultural Science and Technology,2009,(14):276-278.
5.骆畅,刘济明,廖小锋,等.小蓬竹的水分利用效率[J].安徽大学学报:自然科学版,2017,41(2):100-108.Luo C,Liu J M,Liao X F,et al.The water use efficiency of Drepanostachyum luodianense[J].Journal of Anhui University:Natural Science Edition,2017,41(2):100-108.
6.李丽霞,刘济明,廖小锋,等.小蓬竹光合作用对CO₂的响应特征[J].东北林业大学学报,2016,44(8):18-23,54.Li L X,Liu J M,Liao X F,et al.Effect of Drepanostachyum luodianense photosynthesis on characteristic of response to CO₂[J].Journal of Northeast Forestry University,2016,44(8):18-23,54.
7.李鹏,刘济明,文爱华,等.小蓬竹根际土壤可培养细菌多样性[J].江苏农业科学,2016,44(7):223-227.Li P,Liu J M,Wen A H,et al.Diversity of culturable bacteria in rhizosphere soil of Drepanostachyum luodianense[J].Jiangsu Agricultural Sciences,2016,44(7):223-227.
8.池馨,刘济明,颜强,等.小蓬竹群落优势种群的生态位特征[J].贵州农业科学,2014,42(5):180-183.Chi X,Liu J M,Yan Q,et al.Niche characteristics of dominant populations of Drepanostachyum luodianense[J].Guizhou Agricultural Sciences,2014,42(5):180-183.
9.孟勇,刘国华,王福升.小蓬竹地理分布及生长发育规律研究[J].竹子研究汇刊,2010,29(3):15-20.Meng Y,Liu G H,Wang F S.Geographical distribution and growth pattern of Ampelocalamus luodianensis[J].Journal of Bamboo Research,2010,29(3):15-20.
10.马玉栋,苟光前,孟文艺,等.贵州特有、极危竹种——爬竹、小蓬竹的解剖学研究[J].山地农业生物学报,2012,31(1):20-24.Ma Y D,Gou G Q,Meng W Y,et al.The anatomical characters of the endemic and critically endangered bamboo species-Ampelocalamus scandens and Drepanostachyum luodianense in Guizhou Province[J].Journal of Mountain Agriculture and Biology,2012,31(1):20-24.
11.Zhou M B,Zhong H,Tang D Q.Isolation and characterization of seventy-nine full-length mariner-like transposase genes in the Bambusoideae subfamily[J].Journal of Plant Research,2011,124(5):607-617.
12.Doyle J J,Doyle J L.A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J].Phytochemical Bulletin,1987,19(1):11-15.
13.刘济明,王敏,闫国华,等.濒危竹种小蓬竹(Drepanostachyum luodianense)RAPD-PCR反应体系优化[J].浙江农业学报,2014,26(3):667-674.Liu J M,Wang M,Yan G H,et al.Optimization of RAPD-PCR conditions for a rare and endangered bamboo of Drepanostachyum luodianense[J].Acta Agriculturae Zhejiangensis,2014,26(3):667-674.
14.Yeh F C,Yang R C,Boyle T B J,et al.PopGene,the user-friendly shareware for population genetic analysis[M].Edmonton:Molecular Biology and Biotechnology Center,University of Alberta,1997.
15.Stewart C N JR,Via L E.A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications[J].BioTechniques,1993,14(5):748-749.
16.Tai T H,Tanksley S D.A rapid and inexpensive method for isolation of total DNA from dehydrated plant tissue[J].Plant Molecular Biology Reporter,1990,8(4):297-303.
17.邱福林,王和和,陈洁,等.用于水稻突变体大量筛选的DNA微量快速提取法[J].中国水稻科学,2006,20(3):329-332.Qiu F L,Wang H H,Chen J,et al.A rapid DNA miniprep extraction method for large-scale rice mutant screening[J].Chinese Journal of Rice Science,2006,20(3):329-332.
18.蒋瑶,胡尚连,陈其兵,等.四川省不同地区梁山慈竹RAPD与ISSR遗传多样性研究[J].福建林学院学报,2008,28(3):276-280.Jiang Y,Hu S L,Chen Q B,et al.Studies on genetic diversity of Dendrocalamus farinosus from the different regions in Sichuan Province by RAPD and ISSR markers[J].Journal of Fujian College of Forestry,2008,28(3):276-280.
19.Chase M W,Hills H H.Silica gel:an ideal material for field preservation of leaf samples for DNA studies[J].Taxon,1991,40(2):215-220.
20.Ge S,Wang K Q,Hong D Y,et al.Comparisons of genetic diversity in the endangered Adenophora lobophylla and its widespread congener,A.potaninii[J].Conservation Biology,1999,13(3):509-513.
21.王波,高素萍,陈其兵.珍稀濒危观赏竹种筇竹遗传多样性研究[J].竹子研究汇刊,2016,35(1):13-16,21.Wang B,Gao S P,Chen Q B.Genetic diversity of the rare and endangered species Qiongzhuea tumidinoda[J].Journal of Bamboo Research,2016,35(1):13-16,21.
22.滕婕华,卫家贤,李象钦,等.濒危特有种掌叶木的微卫星遗传多样性研究[J].广西植物,2017,37(11):1471-1479.Teng J H,Wei J X,Li X Q,et al.Endangered species endemic to microsatellite genetic diversity study on Handeliodendron bodinieri[J].Guihaia,2017,37(11):1471-1479.
23.段义忠,王建武,亢福仁.孑遗濒危植物蒙古沙冬青ISSR遗传多样性分析[J].分子植物育种,2018,16(15):5008-5014.Duan Y Z,Wang J W,Kang F R.Genetic diversity analysis of endangered relict plant Ammopiptanthus mongolicus by ISSR Markers[J].Molecular Plant Breeding,2018,16(15):5008-5014.
24.Hamrick J L,Godt M J W.Allozyme diversity in plant species[M].//Brown A H D,Clegg M T,Kahler A L,et al.Plant population genetic,breeding,and genetic resource.Sunderland,Massachusetts:Sinauer Associates,1990:43-63.
25.王可青,葛颂,董鸣.根茎禾草沙鞭的等位酶变异及克隆多样性[J].植物学报,1999,41(5):537.Wang K Q,Ge S,Dong M.Allozyme variance and clonal diversity in the rhizomatous grass Psammochloa villosa(Gramineae)[J].Journal of Integrative Plant Biology,1999,41(5):537.
26.Slatkin M.Gene flow and the geographic structure of natural populations[J].Science,1987,236(4803):787-792.
27.Wright S.Evolution in mendelian populations[J].Genetics,1931,16(2):97-159.

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