Cloning and Expression Analysis of Glutelin GluC Gene Promoter in Rice

doi:10.7525/j.issn.1673-5102.2018.06.016

Abstract

Abstract: Rice glutelin, which is expressed in endosperm exclusively, is ideal candidate for isolating promoters with endosperm specificity. We isolated the GluC 5'-flanking sequence pGluC. Analysis of pGluC sequence by PlantCARE and PLACE revealed it contained a series of endosperm specific elements, such as Skn-1 motif and ACGT-box. Thus, we constructed the pGluC and truncated pGluC into pGPTV-GUS vector and transferred the vectors into rice. We performed histochemical assay and GUS activity detection. The full-length and -1 911,-1 611, -1 311 bp promoters were mainly accumulated in endosperm. The -999, -451, -203, -102 bp promoters were mainly accumulated in endosperm, and less strongly in roots, stems and leaves, which changed their endosperm-specific expression pattern. Our findings would contribute to the selection of a suitable promoter for crop molecular improvement.

Key words: rice, promoter, cis-acting element, GUS histochemical staining, quantitative GUS assay

CLC Number:

LI Wen-Jing, SUN Yan-Xiang. Cloning and Expression Analysis of Glutelin GluC Gene Promoter in Rice[J]. Bulletin of Botanical Research, 2018, 38(6): 921-930.

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