Analysis of BpBEE1 Gene Promoter in Betula platyphylla

doi:10.7525/j.issn.1673-5102.2018.02.012

Abstract

Abstract: Brassinolide Enhanced Expression(BEE) genes belong to bHLH transcription factors family, and were involved in Brassinolide signal pathway. In this study, the promoter of BpBEE1 gene was cloned, and regulatory elements in the promoter regions were analyzed using the online program PLACE. The results showed that the BEE1 promoter sequence contained various hormone responses elements. The results showed that the GUS activity was increased in the veins and roots, and the activity of promoter was enhanced after MeJA, SA, BL and ABA treatment, especially MeJA, SA and BL treatment. We propose that BpBEE1 is involved in hormone response, and play a role in the process of plant growth and development.

Key words: Betula platyphylla, BpBEE promoter, Genetic transformation, honmones

CLC Number:

S792

XU Si-Jia, YAN Bin, DONG Jing-Xiang, WU Dan-Yang, LI Hui-Yu. Analysis of BpBEE1 Gene Promoter in Betula platyphylla[J]. Bulletin of Botanical Research, 2018, 38(2): 247-253.

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