Cloning and Expression Analysis of Promoter of CESA7 Gene in Betula platyphylla

doi:10.7525/j.issn.1673-5102.2018.04.009

Abstract

Abstract: A cellulosic synthase gene Betula platyphylla(CESA7) GenBank ID(EU591531) promoter was cloned and analyzed. By sequence analysis, the promoter was found to contain multiple cis-acting elements with different functions, including response elements, hormone-responsive elements, leaf morphology development elements, etc. It was speculated that this promoter plays a key role in the growth and development of birch. We cloned the promoter of BpCESA7 into a plant expression vector with a GUS reporter gene, named proBpCESA7-121-GUS, and invaded B.platyphylla and Arabidopsis thaliana by Agrobacterium-mediated method, and then observed that BpCESA7 gene was activated by GUS histochemical staining. Sub-organizational expression characteristics. Results GUS activity was detected in the roots, stems and leaves of B.platyphylla and Arabidopsis roots, leaves, sepals, and stamens, indicating that the promoter of BpCESA7 gene had promoter activity and was stained the most in the roots and leaves of birch, indicating BpCESA7 genes are highly expressed in the birch roots and leaves, and their tissue-specific expression is present.

Key words: Betula platyphylla, CESA7, promoter, expression, GUS staining

CLC Number:

S792.153

ZHU De-Cai, HU Xiao-Qing, ZHAO Tong, TIAN Jing, ZHANG Yong, LIU Xue-Mei. Cloning and Expression Analysis of Promoter of CESA7 Gene in Betula platyphylla[J]. Bulletin of Botanical Research, 2018, 38(4): 551-558.

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