兰州百合响应自毒胁迫的代谢组学分析

doi:10.7525/j.issn.1673-5102.2024.05.016

摘要/Abstract

摘要：

为探究兰州百合（Lilium davidii var. unicolor）响应自毒胁迫的代谢机制，以1年生（Lily1）、2年生（Lily2）和3年生（Lily3）兰州百合为研究对象，采用气相色谱-质谱（GC-MS）技术分析了不同连作年限兰州百合的代谢组学变化，在其提取物中共检测和鉴定出124种代谢物。利用R软件对样品进行归一化，通过正交偏最小二乘判别分析（OPLS-DA）寻找差异代谢物，并筛选相关代谢物途径进行联合分析。结果表明：Lily1与Lily2的代谢组间存在明显差异，共有16种呈上调的差异代谢物（脂质类、黄酮类、糖类及酚类），且均上调；显著富集至酪氨酸代谢、丙酸代谢和类黄酮生物合成代谢通路。其中脂质类、黄酮类和糖类物质含量的提升表明在兰州百合连作的第2年，自身通过积极合成脂质类、黄酮类和糖类物质来响应自毒胁迫。Lily1与Lily3的代谢组间共有21种差异代谢物，包括1种上调代谢物（3-α-甘露二糖）和20种下调代谢物（胺类、酸类和脂质类）；显著富集至5条代谢通路，分别为托烷、哌啶和吡啶生物碱的生物合成、磷酸盐和次磷酸盐的代谢、赖氨酸降解、谷胱甘肽代谢和D-氨基酸代谢。Lily2与Lily3组间同样有21种差异代谢物，唯一上调代谢物也为3-α-甘露二糖。这表明，在兰州百合连作的第3年，糖类物质作为直接参与维持细胞膜稳定性的碳水化合物，积极响应自毒胁迫。20种下调代谢物分别为胺类、酸类和脂质类，仅显著富集至丙酸代谢和类黄酮生物合成代谢通路。

关键词: 兰州百合, 代谢组, 自毒, GC-MS

Abstract:

In order to explore the metabolic mechanism of Lanzhou lily（Lilium davidii var. unicolor） in response to autotoxicity stress， one-year-old（Lily1）， two-year-old（Lily2） and three-year-old（Lily3） Lanzhou lilies were used as materials， and gas chromatography-mass spectrometry（GC-MS） was used to analyze the metabolomic changes of Lanzhou lily under continuous cropping years， and a total of 124 metabolites were detected and identified. R software was used to normalize the samples， and orthogonal partial least squares discriminant analysis（OPLS-DA） was used to find differential metabolites， and related metabolite pathways were screened for joint analysis. The results showed that there were 16 differential metabolites（lipids， flavonoids， saccharides and phenols） between Lily1 and Lily2， and all of them were up-regulated； and it was significantly enriched in tyrosine metabolism， propionic acid metabolism and flavonoid biosynthetic metabolic pathways. The contents of lipids， flavonoids and saccharides increased， indicating that in the second year of continuous cropping， Lanzhou lily responded to autotoxicity stress by actively synthesizing lipids， flavonoids and saccharides. There were 21 differential metabolites between the metabolomes of Lily1 and Lily3， including one up-regulated metabolite（3-α-mannobiose） and 20 down-regulated metabolite packages （amines， acids， and lipids）； and five metabolic pathways were significantly enriched， including biosynthesis of tropane， piperidine and pyridine alkaloids， phosphate and hypophosphite metabolism， lysine degradation， glutathione metabolism and D-amino acid metabolism. There were 21 differential metabolites between Lily2 and Lily3 groups， and the only up-regulated metabolite was 3-α-mannobiose. The results showed that in the third year of continuous cropping of Lanzhou lily， saccharides， as carbohydrates， were directly involved in cell membrane stability， actively responded to autotoxicity stress. The 20 down-regulated metabolites were amines， acids and lipids； only the propionic acid metabolism and flavonoid biosynthetic metabolism pathways were significantly enriched.

Key words: Lilium davidii var. unicolor, metabolomics, autotoxicity, GC-MS

中图分类号:

Q945

成志远, 张燕江, 申岳, 李广, 陈勤, 李杰, 邢彦宏. 兰州百合响应自毒胁迫的代谢组学分析[J]. 植物研究, 2024, 44(5): 793-804.

Zhiyuan CHENG, Yanjiang ZHANG, Yue SHEN, Guang LI, Qin CHEN, Jie LI, Yanhong XING. Metabolomics Analysis of Lilium davidii var. unicolor in Response to Autotoxicity Stress[J]. Bulletin of Botanical Research, 2024, 44(5): 793-804.

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分组 Group	OPLS-DA模型评价参数 OPLS-DA model evaluation parameters			OPLS-DA验证模型参数 OPLS-DA validation model parameters
分组 Group	R²X	R²Y	Q²	R²X	R²Y	Q²
L1	0.159	0.898	0.523	0.307	0.973	0.684
L2	0.230	0.645	0.439	0.720	0.998	0.705
L3	0.222	0.668	0.503	0.704	0.999	0.747

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