植物研究 ›› 2024, Vol. 44 ›› Issue (1): 118-131.doi: 10.7525/j.issn.1673-5102.2024.01.014
收稿日期:
2023-04-18
出版日期:
2024-01-20
发布日期:
2023-12-27
通讯作者:
安黎哲
E-mail:anlizhe@bjfu.edu.cn
作者简介:
E-mail:anlizhe@bjfu.edu.cn。基金资助:
Received:
2023-04-18
Online:
2024-01-20
Published:
2023-12-27
Contact:
Lizhe AN
E-mail:anlizhe@bjfu.edu.cn
摘要:
为探究油菜素内酯(BRs)诱导提高植物的抗寒性是否受一氧化氮(NO)信号分子调控,以高山离子芥(Chorispora bungeana)悬浮细胞为材料,分别用24-表油菜素内酯(EBR)、NO供体SNP、NO清除剂PTIO、一氧化氮合成酶(NOS)抑制剂L-NAME、EBR+PTIO以及EBR+L-NAME进行处理,分析在低温胁迫下以上处理对细胞抗寒性、活性氧(ROS)水平以及抗氧化防御系统的影响。结果表明:(1)外源EBR处理可以缓解低温胁迫对悬浮细胞活力的抑制以及离子渗漏和膜脂过氧化程度的加剧,从而增强细胞对低温的抗逆能力。SNP处理对上述生理指标的影响与EBR类似。(2)在低温胁迫下,与EBR处理细胞相比,EBR+PTIO以及EBR+L-NAME处理会导致悬浮细胞活力下降,离子渗漏和膜脂过氧化程度显著升高,表明阻断NO信号会降低EBR诱导提高的抗寒性。(3)与仅受低温胁迫的细胞相比,外源EBR处理导致细胞NO含量和NOS活性进一步升高,而EBR诱导的NO积累可以被PTIO或L-NAME所抑制。(4)EBR、SNP处理均可以明显抑制低温胁迫下离子芥悬浮细胞过氧化氢(H2O2)含量、超氧阴离子(
中图分类号:
刘亚洁, 安黎哲. 一氧化氮参与调控油菜素内酯增强高山离子芥悬浮细胞抗寒性[J]. 植物研究, 2024, 44(1): 118-131.
Yajie LIU, Lizhe AN. Nitric Oxide Mediates Brassinosteroids-induced Chilling Tolerance in Chorispora bungeana Suspension Cultured Cells[J]. Bulletin of Botanical Research, 2024, 44(1): 118-131.
图5
低温胁迫下,EBR、SNP、PTIO以及L-NAME处理对高山离子芥悬浮细胞H2O2质量摩尔浓度(A)、O2·–产生速率(B)、OH–含量(C)的影响CK.对照,细胞不加任何化学试剂处理并培养于25 ℃;C.低温胁迫;C+E.低温胁迫+0.1 μmol·L-1 EBR;C+S.低温胁迫+100 μmol·L-1 SNP;C+E+P.低温胁迫+0.1 μmol·L-1 EBR+100 μmol·L-1 PTIO;C+E+L.低温胁迫+0.1 μmol·L-1 EBR+300 μmol·L-1 L-NAME;C+P.低温胁迫+100 μmol·L-1 PTIO;C+L.低温胁迫+300 μmol·L-1 L-NAME;下同。
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