植物研究 ›› 2024, Vol. 44 ›› Issue (1): 107-117.doi: 10.7525/j.issn.1673-5102.2024.01.013
宋泊沂1,2,3,4, 王明明1,2,3,4, 庄伟伟1,2,3,4()
收稿日期:
2023-06-14
出版日期:
2024-01-20
发布日期:
2023-12-27
通讯作者:
庄伟伟
E-mail:zww8611@sina.com
作者简介:
E-mail:zww8611@sina.com。基金资助:
Boyi SONG1,2,3,4, Mingming WANG1,2,3,4, Weiwei ZHUANG1,2,3,4()
Received:
2023-06-14
Online:
2024-01-20
Published:
2023-12-27
Contact:
Weiwei ZHUANG
E-mail:zww8611@sina.com
摘要:
苔藓植物是地表生态系统的重要组分,研究苔藓植物对氮沉降的生理响应可以在机理机制上探讨如何科学合理利用苔藓指示大气氮沉降。以西北地区3种苔藓植物——齿肋赤藓(Syntrichia caninervis),真藓(Bryum argenteum)和尖叶匐灯藓(Plagiomnium acutum)为研究对象,设置0、2、4、6 g·m-2 4个不同氮素处理梯度(分别计为N0、N2、N4、N6),研究氮素增加对不同苔藓植物叶绿素、渗透调节物质含量和抗氧化酶活性的影响。结果表明:(1)4个氮素处理水平均能促进尖叶匐灯藓的叶绿素a和叶绿素b合成,尤以N4处理的促进作用最佳,而N2处理对齿肋赤藓和真藓的叶绿素a和叶绿素b具有明显的抑制作用(对齿肋赤藓的抑制作用更强)。(2)苔藓体内可以产生脯氨酸(Pro),可溶性糖(SS)和可溶性蛋白(SP)来调节细胞渗透平衡。不同氮素处理均促进了真藓和尖叶匐灯藓Pro,SS和SP的含量,但N2处理下齿肋赤藓的3种物质含量开始下降,说明其对氮较为敏感。(3)在设定的氮素添加处理下,低浓度氮素促进3种苔藓植物的抗氧化酶活性,但高浓度氮素抑制苔藓植物的抗氧化酶活性。(4)齿肋赤藓的抗氧化系统调节中起主要作用的是超氧化物歧化酶(SOD),而真藓和尖叶匐灯藓的抗氧化系统调节中起主要作用的是过氧化氢酶(CAT)。综上所述,3种苔藓植物中,齿肋赤藓对氮素增加最为敏感,其次是真藓和尖叶匐灯藓,据此可将齿肋赤藓作为大气氮沉降的指示植物。
中图分类号:
宋泊沂, 王明明, 庄伟伟. 3种苔藓植物对模拟大气氮沉降的生理响应[J]. 植物研究, 2024, 44(1): 107-117.
Boyi SONG, Mingming WANG, Weiwei ZHUANG. Physiological Responses of Three Bryophytes to Simulated Atmospheric Nitrogen Deposition[J]. Bulletin of Botanical Research, 2024, 44(1): 107-117.
表1
供试苔藓的基本信息
科 Family | 物种 Species | 采样地 Sampling site | 生境 Habitat | 经纬度 Latitude and longitude | 海拔 Altitude/m |
---|---|---|---|---|---|
丛藓科 Pottiaceae | 齿肋赤藓 S. caninervis | 古尔班通古特沙漠 Gurbantunggut Desert | 沙漠土壤 Desert soil | 44°22ˊN,87°55ˊE | 395 |
真藓科 Bryaceae | 真藓 B. argenteum | 喀纳斯自然保护区 Kanas Nature Reserve | 湿润沃土 Wet fertile soil | 48°51ˊN,87°13ˊE | 1 340 |
提灯藓科 Mniaceae | 尖叶匐灯藓 P. acutum | 西天山自然保护区 West Tianshan Nature Reserve | 云杉林下 Under spruce understory | 43°10ˊN,82°52ˊE | 2 240 |
表2
供试3种生境的气象和土壤养分信息
采样地 Sampling site | 养分信息 Nutrient information | 气象信息 Meteorological information | ||||
---|---|---|---|---|---|---|
有机碳 Organic carbon /(g·kg-1) | 全氮 Total N/(g·kg-1) | 全磷 Total P/(g·kg-1) | 年均温 Mean annual temperature/℃ | 年降水 Mean annual precipitation/mm | 干旱程度 Aridity | |
古尔班通古特沙漠 Gurbantunggut Desert | 7.725 | 0.667 | 0.630 | 10.60 | 123.02 | 0.895 |
喀纳斯自然保护区 Kanas Nature Reserve | 83.243 | 3.091 | 0.892 | 8.20 | 150.30 | 0.725 |
西天山自然保护区 West Tianshan Nature Reserve | 40.970 | 1.948 | 0.827 | 9.50 | 136.60 | 0.822 |
1 | GAO Y, JIA Y L, YU G R,et al.Anthropogenic reactive nitrogen deposition and associated nutrient limitation effect on gross primary productivity in inland water of China[J].Journal of Cleaner Production,2019,208:530-540. |
2 | XU W, ZHAO Y H, LIU X J,et al.Atmospheric nitrogen deposition in the Yangtze River basin:spatial pattern and source attribution[J].Environmental Pollution,2018,232:546-555. |
3 | LI K H, LIU X J, SONG W,et al.Atmospheric Nitrogen Deposition at Two Sites in an Arid Environment of Central Asia[J].PLoS One,2013,8(6):e67018. |
4 | BAI Y F, WU J G, CLARK C M,et al.Tradeoffs and thresholds in the effects of nitrogen addition on biodiversity and ecosystem functioning:evidence from inner Mongolia Grasslands[J].Global Change Biology,2010,16(1):358-372. |
5 | ZHANG Y, ZHENG L X, LIU X J,et al.Evidence for organic N deposition and its anthropogenic sources in China[J].Atmospheric Environment,2008,42(5):1035-1041. |
6 | XU W, LUO X S, PAN Y P,et al.Quantifying atmospheric nitrogen deposition through a nationwide monitoring network across China[J].Atmospheric Chemistry and Physics,2015,15(21):12345-12360. |
7 | JING Y L, GUAN D X, WU J B,et al.Photosynthate supply drives soil respiration of Fraxinus mandshurica seedlings in northeastern China:evidences from a shading and nitrogen addition experiment[J].Journal of Forestry Research,2016,27(6):1271-1276. |
8 | LI G, ZHANG Z S, GAO H Y,et al.Effects of nitrogen on photosynthetic characteristics of leaves from two different stay-green corn(Zea mays L.) varieties at the grain-filling stage[J].Canadian Journal of Plant Science,2012,92(4):671-680. |
9 | WANG C, LI X N, HU Y X,et al.Nitrogen addition weakens the biodiversity-multifunctionality relationships across soil profiles in a grassland assemblage[J].Agriculture,Ecosystems and Environment,2023,342:108241. |
10 | 朱瑞良,马晓英,曹畅,等.中国苔藓植物多样性研究进展[J].生物多样性,2022,30(7):22378. |
ZHU R L,MA X Y,CAO C,et al,Advances in research on bryophyte diversity in China[J].Biodiversity Science,2022,30(7):22378. | |
11 | POTT U, TURPIN D H.Changes in atmospheric trace element deposition in the Fraser Valley,B.C.,Canada from 1960 to 1993 measured by moss monitoring with Isothecium stoloniferum[J].Canadian Journal of Botany,1996,74(8):1345-1353. |
12 | WOLTERBEEK H T, KUIK P, VERBURG T G,et al.Moss interspecies comparisons in trace element concentrations[J].Environmental Monitoring and Assessment,1995,35(3):263-286. |
13 | STORKEY J, MACDONALD A J, POULTON P R,et al.Grassland biodiversity bounces back from long-term nitrogen addition[J].Nature,2015,528(7582):401-404. |
14 | GONZÁLEZ-MIQUEO L, ELUSTONDO D, LASHERAS E,et al.Spatial trends in heavy metals and nitrogen deposition in Navarra(Northern Spain) based on moss analysis[J].Journal of Atmospheric Chemistry,2010,62(1):59-72. |
15 | AGNAN Y, SÉJALON-DELMAS N, CLAUSTRES A,et al.Investigation of spatial and temporal metal atmospheric deposition in France through lichen and moss bioaccumulation over one century[J].Science of the Total Environment,2015,529:285-296. |
16 | 董向楠.氮素添加对山西太岳山苔藓植物的影响[D].北京:北京林业大学,2016:29-31. |
DONG X N.Effects of nitrogen addition on bryophytes in Taiyue Mountain of Shanxi[D].Beijing:Beijing Forestry University,2016:29-31. | |
17 | BRITTON A J, MITCHELL R J, FISHER J M,et al.Nitrogen deposition drives loss of moss cover in alpine moss-sedge heath via lowered C∶N ratio and accelerated decomposition[J].New Phytologist,2018,218(2):470-478. |
18 | 周晓兵,尹本丰,张元明.模拟氮沉降对不同类型生物土壤结皮生长和光合生理的影响[J].生态学报,2016,36(11):3197-3205. |
ZHOU X B, YIN B F, ZHANG Y M.The effects of simulated nitrogen deposition on growth and photosynthetic physiology of three types of biocrusts[J].Acta Ecologica Sinica,2016,36(11):3197-3205 | |
19 | 刘滨扬,刘蔚秋,雷纯义,等.三种苔藓植物对模拟N沉降的生理响应[J].植物生态学报,2009,33(1):141-149. |
LIU B Y, LIU W Q, LEI C Y,et al.Physiological responses of three bryophyte species of south china to simulated nitrogen deposition[J].Chinese Journal of Plant Ecology,2009,33(1):141-149. | |
20 | 热比也木·吾甫,艾尼瓦尔·阿布都热衣木,玛尔孜亚·阿不力米提,等.新疆苔藓植物的研究进展[J].新疆大学学报(自然科学版),2014,31(3):335-340,362. |
RABIYE GUPUR, ANWAR ABDUREHIM, MARZIYA ABLIMIT,et al.Recent advances in Xinjiang Bryoligical Research[J].Journal of Xinjiang University(Natural Science Edition),2014,31(3):335-340,362. | |
21 | KIDRON G J.The negative effect of biocrusts upon annual-plant growth on sand dunes during extreme droughts[J].Journal of Hydrology,2014,508(1):128-136. |
22 | ZHUANG W W, DOWNING A, ZHANG Y M.The influence of biological soil crusts on 15N translocation in soil and vascular plant in a temperate desert of Northwestern China[J].Journal of Plant Ecology,2015,8(4):420-428. |
23 | 李俊柯,杜家豪,邓章轩,等.铀胁迫对不同苔藓生长及抗氧化系统的影响[J].广东农业科学,2020,47(8):65-73. |
LI J K, DU J H, DENG Z X,et al.Effects of Uranium stress on growth and antioxidant system of different Bryophytes[J].Guangdong Agricultural Sciences,2020,47(8):65-73. | |
24 | 刘滨扬,刘蔚秋,张以顺,等.低温胁迫后苔藓植物对模拟氮沉降条件的生理响应[J].植物生态学报,2011,35(3):268-274. |
LIU B Y, LIU W Q, ZHANG Y C,et al.Physiological responses of bryophytes experienced low temperature stress to simulated nitrogen deposition[J].Chinese Journal of Plant Ecology,2011,35(3):268-274. | |
25 | ASGHAR T, JAMIL Y, IQBAL M,et al.Laser light and magnetic field stimulation effect on biochemical,enzymes activities and chlorophyll contents in soybean seeds and seedlings during early growth stages[J].Journal of Photochemistry and Photobiology B:Biology,2016,165:283-290. |
26 | BATES L S, WALDREN R P, TEARE I D.Rapid determination of free proline for water-stress studies[J].Plant and Soil,1973,39:205-207. |
27 | GIANNAKOULA A, MOUSTAKAS M, SYROS T,et al.Aluminum stress induces up-regulation of an efficient antioxidant system in the Al-tolerant maize line but not in the Al-sensitive line[J].Environmental and Experimental Botany,2010,67(3):487-494. |
28 | LASSOUANE N, AÏD F, LUTTS S.Water stress impact on young seedling growth of Acacia arabica [J].Acta Physiologiae Plantarum,2013,35:2157-2169. |
29 | HEATH R L, PACKER L.Photoperoxidation in isolated chloroplasts:I.Kinetics and stoichiometry of fatty acid peroxidation[J].Archives of Biochemistry and Biophysics,1968,125(1):189-198. |
30 | WU H L, WU X L, LI Z H,et al.Physiological evaluation of drought stress tolerance and recovery in cauliflower (Brassica oleracea L.) seedlings treated with methyl jasmonate and coronatine[J].Journal of Plant Growth Regulation,2012,31(1):113-123. |
31 | ZHOU X B, ZHANG Y M, JI X H,et al.Combined effects of nitrogen deposition and water stress on growth and physiological responses of two annual desert plants in northwestern China[J].Environmental and Experimental Botany,2011,74:1-8. |
32 | OCHOA-HUESO R, MEJÍAS-SANZ V, PÉREZ-CORONA M E,et al.Nitrogen deposition effects on tissue chemistry and phosphatase activity in Cladonia foliacea (Huds.) Willd.,a common terricolous lichen of semi-arid Mediterranean shrublands[J].Journal of Arid Environments,2013,88:78-81. |
33 | ZHOU X B, ZHANG Y M, NIKLAS K J.Sensitivity of growth and biomass allocation patterns to increasing nitrogen:a comparison between ephemerals and annuals in the Gurbantunggut Desert,north-western China[J].Annals of Botany,2014,113(3):501-511. |
34 | REICH P B.Elevated CO2 reduces losses of plant diversity caused by nitrogen deposition[J].Science,2009,326(5958):1399-1402. |
35 | JOHANSSON O, OLOFSSON J, GIESLER R,et al.Lichen responses to nitrogen and phosphorus additions can be explained by the different symbiont responses[J].New Phytologist,2011,191(3):795-805. |
36 | LANGE O L, NOBEL P S, OSMOND C B,et al.Physiological plant ecology I:Responses to the physical environment[M].New York:Springer,1981. |
37 | KITAJIMA K, HOGAN K P.Increases of chlorophyll a/b ratios during acclimation of tropical woody seedlings to nitrogen limitation and high light[J].Plant,Cell & Environment,2003,26(6):857-865. |
38 | LIU B Y, LEI C Y, JIN J H,et al.Physiological responses of two moss species to the combined stress of water deficit and elevated N deposition (II):Carbon and nitrogen metabolism[J].Ecology and Evolution,2016,6(21):7596-7609. |
39 | ZHANG Y M, ZHOU X B, YIN B F,et al.Sensitivity of the xerophytic moss Syntrichia caninervis to prolonged simulated nitrogen deposition[J].Annals of Botany,2016,117(7):1153-1161. |
40 | BELNAP J, PHILLIPS S L, FLINT S,et al.Global change and biological soil crusts:effects of ultraviolet augmentation under altered precipitation regimes and nitrogen additions[J].Global Change Biology,2008,14(3):670-686. |
41 | 周晓兵,张元明,陶冶,等.新疆古尔班通古特沙漠土壤N2O、CH4和CO2通量及其对氮沉降增加的响应[J].植物生态学报,2017,41(3):290-300. |
ZHOU X B, ZHANG Y M, TAO Y,et al.Effluxes of nitrous oxide,methane and carbon dioxide and their responses to increasing nitrogen deposition in the Gurbantünggüt Desert of Xinjiang,China[J].Chinese Journal of Plant Ecology,2017,41(3):290-300. | |
42 | BOBBINK R, HORNUNG M, ROELOFS J G M.The effects of air-borne nitrogen pollutants on species diversity in natural and semi-natural European vegetation[J].Journal of Ecology,1998,86(5):717-738. |
43 | 匡鹤凌,汪贵斌,曹福亮.氮素对喜树光合作用、营养元素和喜树碱含量的影响[J].南京林业大学学报(自然科学版),2016,40(3):15-20. |
KANG H L, WANG G B, CAO F L.Influence of nitrogen levels on photosynthesis,nutrient elements and camptothecin content of Camptotheca acuminata [J].Journal of Nanjing Forestry University(Natural Sciences Edition),2016,40(3):15-20. |
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