植物研究 ›› 2023, Vol. 43 ›› Issue (5): 700-710.doi: 10.7525/j.issn.1673-5102.2023.05.007
江转转, 龚莉, 宋亚玲
收稿日期:
2023-01-07
出版日期:
2023-09-20
发布日期:
2023-09-05
作者简介:
江转转(1991 —),女,博士,主要从事植物抗逆分子遗传学研究。
基金资助:
Zhuanzhuan JIANG, Li GONG, Yaling SONG
Received:
2023-01-07
Online:
2023-09-20
Published:
2023-09-05
About author:
JIANG Zhuanzhuan(1991—),female,doctor,engaged in the research of molecular genetics of plant resistance.
Supported by:
摘要:
在DNA、RNA及细胞水平上鉴定了叶绿体异常分裂纯合突变株parc6,子叶白化纯合突变株sco2,同时以真叶异常分裂及子叶白化双重突变株sl2作为参照,通过向培养基中施加不同浓度的蔗糖含量来探究叶绿体异常分裂对子叶和真叶生长的影响。结果显示,sco2突变株子叶白化,而真叶生长正常;parc6突变株子叶生长势及生活力显著低于野生型对照,相当于sco2突变株,而子叶白化与真叶异常分裂双重突变株sl2生长严重受阻。parc6突变株真叶的生长势与生活力也显著低于野生型,但相较于子叶则有所恢复。parc6突变株子叶生长受阻表型可以通过在培养基上补充碳源而得到恢复,sco2突变株子叶生长受阻表型可以通过补充碳源得到恢复,但叶绿体荧光参数相较于野生型仍有差异。蓝色温和凝胶电泳结合二向聚丙烯酰胺凝胶电泳结果表明parc6突变株的子叶及真叶光系统高级结构组装正常,说明parc6异常分裂的叶绿体无法生成足够的能量进而影响子叶及真叶的生长。进化分析表明,PARC6与SCO2存在共同进化的趋势,说明子叶发育与叶绿体分裂存在一定的联系。结果指示,叶绿体大小与植物生长尤其是子叶的发育存在密切的联系,这为深入揭示叶绿体的功能提供一个新的视角。
中图分类号:
江转转, 龚莉, 宋亚玲. 拟南芥叶绿体分裂蛋白PARC6影响子叶与真叶的生长[J]. 植物研究, 2023, 43(5): 700-710.
Zhuanzhuan JIANG, Li GONG, Yaling SONG. The Chloroplast Division Protein PARC6 Affected the Growth of Cotyledon and Leaf in Arabidopsis thaliana[J]. Bulletin of Botanical Research, 2023, 43(5): 700-710.
表1
研究中所用引物序列
基因名称 Gene name | 引物序列5′→3′ Primer sequence 5′→3′ | |
---|---|---|
PCR引物 PCR primer | SCO2 | F:CTTCTTCCTCCATTTTCGTTACACCT |
SCO2 | R:GTCCATTGTTGAGTTTCACATT | |
PARC6 | LP:TCTCGCACATTAGTTATGGGC | |
PARC6 | RP:ATAGGTCTTAACCTGGCGAGC | |
PARC6 | TP:ATTTTGCCGATTTCGGAAC | |
QPCR引物 QPCR primer | SCO2 | F:AATGGCTTCTTCTTTCACCAATAGTC |
SCO2 | R:GGTGTCTCGAGGTCTGATACATATAAAA | |
PARC6 | F:GTCCTTGATGAATCCATGCTTGTCCAG | |
PARC6 | R:GAAGAGCTTCGATTTCTGCAGCCTCACC | |
ACTIN2 | F:CCCGATGGGCAAGTCATC | |
ACTIN2 | R:GAACAAGACTTCTGGGCATCTGA |
图1
sco2、parc6突变株基因分型及表达量A.SCO2基因结构模型及DNA序列突变位点(白色方框代非翻译区,绿色方框代表外显子,线条代表内含子);B.生长8 d的野生型与sco2突变株的表型;C.野生型和sco2突变株基因组的基因分型及PCR分析,引物位置在图A中指示;D.SCO2基因在野生型、sco2突变株中的表达量;E.parc6中T-DNA插入位置及基因结构示意图(白色方框代非翻译区,绿色方框代表外显子,线条代表内含子);F.生长24 d的野生型与parc6突变株的表型;G.野生型和parc6突变株基因组的基因分型及PCR分析,引物位置在图D中显示;H.PARC6基因在野生型、parc6突变株中的表达量
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