拟南芥AtUNE12基因的耐盐功能初探

doi:10.7525/j.issn.1673-5102.2020.02.013

摘要/Abstract

摘要： bHLH转录因子家族成员在植物生长发育、生理代谢及非生物胁迫响应过程中起重要作用。本研究选取拟南芥抗逆相关bHLH转录因子家族中AtUNE12基因为研究对象，对其进行耐盐功能初探。首先构建AtUNE12基因的植物过表达载体（pROKⅡ-AtUNE12），通过农杆菌介导的浸花法转化拟南芥，利用qRT-PCR技术检测获得T₃代AtUNE12过表达转基因植株。在盐胁迫下，分析过表达AtUNE12与野生型拟南芥长势、根长及鲜重；比较过表达AtUNE12与野生型植株的电解质渗透率、失水率、MDA含量、POD与SOD活性及H₂O₂含量，鉴定AtUNE12基因是否具有耐盐能力。结果表明：过表达AtUNE12基因降低了拟南芥植株的失水率、电解质渗透率及MDA含量，保护细胞膜结构的完整性；增强了POD与SOD活性，降低了拟南芥植株内的H₂O₂含量，进而增强拟南芥植株的ROS清除能力，从而提高拟南芥的耐盐能力。

关键词: bHLH转录因子, AtUNE12, 拟南芥, 耐盐, 抗逆分析

Abstract: Members of the bHLH transcription factor family plays an important role in plant growth, metabolism and abiotic stress response. The salt tolerance of AtUNE12 gene that was part of stress-related bHLH transcription factor family from Arabidopsis thaliana was analyzed. Firstly, the plant overexpression vector of AtUNE12(pROKII-AtUNE12) was constructed, the transgenic T₃ generation A.thaliana was obtained by Agrobacterium-mediated dip then verified by qRT-PCR. Under salt stress, the growth, root length and fresh weight of overexpression and wild-type A.thaliana were analyzed. The MDA content, electrolyte leakage and water loss rate of overexpression and wild-type A.thaliana were compared. H₂O₂ content determination and SOD, POD activity was used to study overexpression and wild-type A.thaliana plants. The results showed that overexpression of AtUNE12 gene could significantly decrease MDA content, electrolyte leakage and the water loss rate, improved POD and SOD activity, and decreased H₂O₂ content in A.thaliana plants. The results indicated that AtUNE12 gene protected the integrity of cell membrane structure, and then enhanced the ROS scavenging ability of A.thaliana plants, thereby improving the salt tolerance of A.thaliana.

Key words: bHLH transcription factor, AtUNE12, Arabidopsis thaliana, salt tolerance, stress tolerance analysis

中图分类号:

Q943.2

李子义, 贺子航, 卢惠君, 王玉成, 及晓宇. 拟南芥AtUNE12基因的耐盐功能初探[J]. 植物研究, 2020, 40(2): 257-265.

LI Zi-Yi, HE Zi-Hang, LU Hui-Jun, WANG Yu-Cheng, JI Xiao-Yu. Study on Salt Tolerance of AtUNE12 Gene in Arabidopsis thaliana[J]. Bulletin of Botanical Research, 2020, 40(2): 257-265.

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