植物研究 ›› 2018, Vol. 38 ›› Issue (4): 632-640.doi: 10.7525/j.issn.1673-5102.2018.04.020
• 研究简报 • 上一篇
于婷婷, 倪秀珍, 高立宏, 韩国军, 朱长甫, 盛彦敏
收稿日期:
2017-12-04
出版日期:
2018-07-15
发布日期:
2018-07-21
通讯作者:
盛彦敏
E-mail:yms_89@sina.com
作者简介:
于婷婷(1993-),女,硕士研究生,主要研究方向为植物次生代谢与分子调控。
基金资助:
YU Ting-Ting, NI Xiu-Zhen, GAO Li-Hong, HAN Guo-Jun, ZHU Chang-Fu, SHENG Yan-Min
Received:
2017-12-04
Online:
2018-07-15
Published:
2018-07-21
Supported by:
摘要: 花青素苷是影响植物花瓣呈色的重要色素,而花色是决定花卉观赏价值和商业价值的一个重要因素。在花青素苷的生物合成过程中,二氢黄酮醇4-还原酶(DFR)是花青素苷生物合成下游途径中的第一个关键的酶。因此,DFR在高等植物花色的形成过程中发挥极其重要的作用,是形成花青素苷的一个非常重要的调控点。DFR对3种二氢黄酮醇底物具有选择特异性,但决定DFR底物特异性的分子机制目前仍不十分清楚。该文简单概述了花青素苷生物合成途径及其转录调控机制,并结合作者的工作重点综述了DFR的底物特异性以及克隆的DFR基因在植物基因工程中的应用。
中图分类号:
于婷婷, 倪秀珍, 高立宏, 韩国军, 朱长甫, 盛彦敏. 高等植物二氢黄酮醇4-还原酶基因研究进展[J]. 植物研究, 2018, 38(4): 632-640.
YU Ting-Ting, NI Xiu-Zhen, GAO Li-Hong, HAN Guo-Jun, ZHU Chang-Fu, SHENG Yan-Min. Advances in Study of Dihydroflavonol 4-Reductase(DFR) Genes of Higher Plants[J]. Bulletin of Botanical Research, 2018, 38(4): 632-640.
1. Grotewold E. The genetics and biochemistry of floral pigments[J]. Annual Review of Plant Biology,2006,57:761-780. 2. Tanaka Y,Sasaki N,Ohmiya A. Biosynthesis of plant pigments:anthocyanins,betalains and carotenoids[J]. The Plant Journal,2008,549(4):733-749. 3. Tanaka Y,Brugliera F. Flower colour and cytochromes P450[J]. Philosophical Transactions of the Royal Society B:Biological Sciences,2013,368(1612):20120432,doi:10. 1098/rstb. 2012. 0432. 4. 朱长甫,陈星,王英典. 植物类胡萝卜素生物合成及其相关基因在基因工程中的应用[J]. 植物生理与分子生物学学报,2004,30(6):609-618.Zhu C F,Chen X,Wang Y D. Carotenoid biosynthesis in plants and application of its relative genes in gene engineering[J]. Journal of Plant Physiology and Molecular Biology,2004,30(6):609-618. 5. Zhu C F,Bai C,Sanahuja G,et al. The regulation of carotenoid pigmentation in flowers[J]. Archives of Biochemistry and Biophysics,2010,504(1):132-141. 6. Zhang Y,Butelli E,Martin C. Engineering anthocyanin biosynthesis in plants[J]. Current Opinion in Plant Biology,2014,19:81-90. 7. 倪勤学,霍艳荣,陆国权. 花色苷保健功能的研究进展[J]. 安徽农业科学,2010,38(35):20025-20028,20453.Ni Q X,Huo Y R,Lu G Q. Advances in health functions of anthocyanins[J]. Journal of Anhui Agricultural Science,2010,38(35):20025-20028,20453. 8. 刘仕芸,黄艳岚,张树珍. 植物花青素生物合成中的调控基因[J]. 植物生理学通讯,2006,42(4):747-754.Liu S Y,Huang Y L,Zhang S Z. Regulatory gene of anthocyanin biosynthesis in plant[J]. Plant Physiology Communications,2006,42(4):747-754. 9. 黄鸿曼,袁利兵,彭志红,等. 花青素的生物合成与环境调控研究进展[J]. 湖南农业科学,2011,(13):118-120.Huang H M,Yuan L B,Peng Z H,et al. Advances in biosynthesis and environmental regulation of anthocyanin[J]. Hunan Agricultural Sciences,2011,(13):118-120. 10. Berman J,Sheng Y M,Gómez L G,et al. Red anthocyanins and yellow carotenoids form the color of orange-flower gentian(Gentiana lutea L. var. aurantiaca)[J]. PLoS One,2016,11(9):e0162410,doi:10. 1371/journal. pone. 0162410. 11. Tian J,Han Z Y,Zhang J,et al. The balance of expression of dihydroflavonol 4-reductase and flavonol synthase regulates flavonoid biosynthesis and red foliage coloration in crabapples[J]. Scientific Reports,2015,5:12228,doi:10. 1038/srep12228. 12. 石少川,高亦珂,张秀海,等. 植物花青素生物合成相关基因的研究及应用[J]. 植物研究,2011,31(5):633-640.Shi S C,Gao Y K,Zhang X H,et al. Progress on plant genes involved in biosynthetic pathway of anthocyanins[J]. Bulletin of Botanical Research,2011,31(5):633-640. 13. 罗庆林,邵继荣,胡建平,等. 荞麦中类黄酮的研究进展[J]. 食品研究与开发,2008,29(2):160-164.Luo Q L,Shao J R,Hu J P,et al. Research progress of flavaniods in Buckwheat[J]. Food Research and Development,2008,29(2):160-164. 14. Petit P,Granier T,D'estaintot B L,et al. Crystal structure of grape dihydroflavonol 4-reductase,a key enzyme in flavonoid biosynthesis[J]. Journal of Molecular Biology,2007,368(5):1345-1357. 15. Matsui K,Hisano T,Yasui Y,et al. Isolation and characterization of genes encoding leucoanthocyanidin reductase(FeLAR) and anthocyanidin reductase(FeANR) in buckwheat(Fagopyrum esculentum)[J]. Journal of Plant Physiology,2016,205:41-47. 16. 张龙,李卫华,姜淑梅,等. 花色素苷生物合成与分子调控研究进展[J]. 园艺学报,2008,35(6):909-916.Zhang L,Li W H,Jiang S M,et al. Progress of molecular basis of biosynthesis and transcriptional regulation of anthocyanins[J]. Acta Horticulturae Sinica,2008,35(6):909-916. 17. Davies K M,Schwinn K E,Deroles S C,et al. Enhancing anthocyanin production by altering competition for substrate between flavonol synthase and dihydroflavonol 4-reductase[J]. Euphytica,2003,131(3):259-268. 18. 高燕会,黄春红,朱玉球,等. 植物花青素苷生物合成及调控的研究进展[J]. 中国生物工程杂志,2016,32(8):94-99.Gao Y H,Huang C H,Zhu Y Q,et al. Progress on plant anthocyanin biosynthesis and regulation[J]. China Biotechnology,2016,32(8):94-99. 19. 祝志欣,鲁迎青. 花青素代谢途径与植物颜色变异[J]. 植物学报,2016,51(1):107-119.Zhu Z X,Lu Y C. Plant color mutants and the anthocyanin pathway[J]. Chinese Bulletin of Botany,2016,51(1):107-119. 20. 梁平,宋洪元. 植物花青素生物合成转录调控研究进展[J]. 南方农业学报,2014,45(8):1375-1379.Liang P,Song H Y. Advances on the transcriptional regulation of anthocyanin biosynthesis in plant[J]. Journal of Southern Agriculture,2014,45(8):1375-1379. 21. 梅菊芬,徐德良,汤茶琴,等. 茶树花青素及其种质资源的研究和利用进展[J]. 热带农业工程,2013,37(1):42-46.Mei J F,Xu D L,Tang C Q,et al. Advances on research and utilization of tea tree(Camellia sinensis) anthocyanins and its germplasm resources[J]. Tropical Agricultural Engineering,2013,37(1):42-46. 22. Ramsay N A,Glover B J. MYB-bHLH-WD40 protein complex and the evolution of cellular diversity[J]. Trends in Plant Science,2005,10(2):63-70. 23. Hichri I,Barrieu F,Bogs J,et al. Recent advances in the transcriptional regulation of the flavonoid biosynthetic pathway[J]. Journal of Experimental Botany,2011,62(8):2465-2483. 24. Xu W J,Dubos C,Lepiniec L. Transcriptional control of flavonoid biosynthesis by MYB-bHLH-WDR complexes[J]. Trends in Plant Science,2015,20(3):176-185. 25. Zhu Z X,Wang H L,Wang Y T,et al. Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation[J]. Journal of Experimental Botany,2015,66(13):3775-3789. 26. Hernandez J M,Heine G F,Irani N G,et al. Different mechanisms participate in the R-dependent activity of the R2R3 MYB transcription factor C1[J]. Journal of Biological Chemistry,2004,279(46):48205-48213. 27. Hartmann U,Sagasser M,Mehrtens F,et al. Differential combinatorial interactions of cis-acting elements recognized by R2R3-MYB,BZIP,and BHLH factors control light-responsive and tissue-specific activation of phenylpropanoid biosynthesis genes[J]. Plant Molecular Biology,2005,57(2):155-171. 28. Dare A P,Schaffer R J,Kui L W,et al. Identification of a cis-regulatory element by transient analysis of co-ordinately regulated genes[J]. Plant Methods,2008,4:17,doi:10. 1186/1746-4811-4-17. 29. Wang H L,Guan S,Zhu Z X,et al. A valid strategy for precise identifications of transcription factor binding sites in combinatorial regulation using bioinformatic and experimental approaches[J]. Plant Methods,2013,9(1):34,doi:10. 1186/1746-4811-9-34. 30. Meyer P,Heidmann I,Forkmann G,et al. A new Petunia flower colour generated by transformation of a mutant with a maize gene[J]. Nature,1987,330(6149):677-678. 31. Johnson E T,Yi H,Shin B,et al. Cymbidium hybrida dihydroflavonol 4-reductase does not efficiently reduce dihydrokaempferol to produce orange pelargonidin-type anthocyanins[J]. The Plant Journal,1999,19(1):81-85. 32. Johnson E T,Ryu S,Yi H,et al. Alteration of a single amino acid changes the substrate specificity of dihydroflavonol 4-reductase[J]. The Plant Journal,2001,25(3):325-333. 33. 樊云芳,陈晓军,李彦龙,等. 宁夏枸杞DFR基因的克隆与序列分析[J]. 西北植物学报,2011,31(12):2373-2379.Fan Y F,Chen X J,Li Y L,et al. Cloning and sequence analysis of dihydroflavonol 4-reductase gene from Lycium barbarum[J]. Acta Botanica Boreali-Occidentalia Sinica,2011,31(12):2373-2379. 34. Forkmann G,Ruhnau B. Distinct substrate specificity of dihydroflavonol 4-reductase from flowers of Petunia hybrida[J]. Zeitschrift für Naturforschung C,1987,42(9-10):1146-1148. 35. Beld M,Martin C,Huits H,et al. Flavonoid synthesis in Petunia hybrida:partial characterization of dihydroflavonol-4-reductase genes[J]. Plant Molecular Biology,1989,13(5):491-502. 36. Xie D Y,Jackson L A,Cooper J D,et al. Molecular and biochemical analysis of two cDNA clones encoding dihydroflavonol-4-reductase from Medicago truncatula[J]. Plant Physiology,2004,134(3):979-994. 37. Miosic S,Thill J,Milosevic M,et al. Dihydroflavonol 4-reductase genes encode enzymes with contrasting substrate specificity and show divergent gene expression profiles in Fragaria species[J]. PLoS One,2014,9(11):e112707,doi:10. 1371/journal. pone. 0112707. 38. Sasaki N,Nakayama T. Achievements and perspectives in biochemistry concerning anthocyanin modification for blue flower coloration[J]. Plant and Cell Physiology,2015,56(1):28-40. 39. Nakatsuka T,Abe Y,Kakizaki Y,et al. Production of red-flowered plants by genetic engineering of multiple flavonoid biosynthetic genes[J]. Plant Cell Reports,2007,26(11):1951-1959. 40. Yuan Y W,Rebocho A B,Sagawa J M,et al. Competition between anthocyanin and flavonol biosynthesis produces spatial pattern variation of floral pigments between Mimulus species[J]. Proceedings of National Academy of Sciences of the United States of America,2016,113(9):2448-2453. 41. Luo P,Ning G G,Wang Z,et al. Disequilibrium of flavonol synthase and dihydroflavonol-4-reductase expression associated tightly to white vs. red color flower formation in plants[J]. Frontiers in Plant Science,2016,6:1257,doi:10. 3389/fpls. 2015. 01257. 42. Helariutta Y,Elomaa P,Kotilainen M,et al. Cloning of cDNA coding for dihydroflavonol-4-reductase(DFR) and characterization of dfr expression in the corollas of Gerbera hybrida var. regina(Compositae)[J]. Plant Molecular Biology,1993,22(2):183-193. 43. Tanaka Y,Brugliera F,Chandler S. Recent progress of flower colour modification by biotechnology[J]. International Journal of Molecular Sciences,2009,10(12):5350-5369. 44. Tanaka Y,Tsuda S,Kusumi T. Metabolic engineering to modify flower color[J]. Plant Cell and Physiology,1998,39(11):1119-1126. 45. 周惠,文锦芬,邓明华,等. 植物花青素生物合成相关基因研究进展[J]. 辣椒杂志,2011,(4):1-7.Zhou H,Wen J F,Deng M H,et al. Research progress in plant anthocyanidin biosynthesis genes[J]. Journal of China Capsicum,2011,(4):1-7. 46. Itoh Y,Higeta D,Suzuki A,et al. Excision of transposable elements from the chalcone isomerase and dihydroflavonol 4-reductase genes may contribute to the variegation of the yellow-flowered carnation(Dianthus caryophyllus)[J]. Plant and Cell Physiology,2002,43(5):578-585. 47. Aida R,Yoshida K,Kondo T,et al. Copigmentation gives bluer flowers on transgenic torenia plants with the antisense dihydroflavonol-4-reductase gene[J]. Plant Science,2000,160(1):49-56. 48. Seitz C,Vitten M,Steinbach P,et al. Redirection of anthocyanin synthesis in Osteospermum hybrida by a two-enzyme manipulation strategy[J]. Phytochemistry,2007,68(6):824-833. 49. Nakamura N,Fukuchi-Mizutani M,Fukui Y,et al. Generation of pink flower varieties from blue Torenia hybrida by redirecting the flavonoid biosynthetic pathway from delphinidin to pelargonidin[J]. Plant Biotechnology,2010,27(5):375-383. 50. Lee W S,You J A,Chung H,et al. Molecular cloning and biochemical analysis of dihydroflavonol 4-reductase(DFR) from Brassica rapa ssp. pekinesis(Chinese Cabbage) using a heterologous system[J]. Journal of Plant Biology,2008,51(1),42-47. 51. Huang Y,Gou J Q,Jia Z C,et al. Molecular cloning and characterization of two genes encoding dihydroflavonol-4-reductase from Populus trichocarpa[J]. PLoS One,2012,7(2):e30364,doi:10. 1371/journal. pone. 0030364. 52. 李义龙,肇涛澜,陈立超,等. 花色素苷生物合成及花色的调控[J]. 生命科学,2008,20(1):147-152.Li Y L,Zhao T L,Chen L C,et al. Regulation in pigment biosynthesis and color variation of flowers[J]. Chinese Bulletin of Life Sciences,2008,20(1):147-152. 53. Hayashi M,Takahashi H,Tamura K,et al. Enhanced dihydroflavonol-4-reductase activity and NAD homeostasis leading to cell death tolerance in transgenic rice[J]. Proceedings of National Academy of Sciences of the United States of America,2005,102(19):7020-7025. 54. Kumar V,Nadda G,Kumar S,et al. Transgenic tobacco overexpressing tea cDNA encoding dihydroflavonol 4-reductase and anthocyanidin reductase induces early flowering and provides biotic stress tolerance[J]. PLoS One,2013,8(6):e65535,doi:10. 1371/journal. pone. 0065535. 55. Li Y Q,Liu X X,Cai X Q,et al. Dihydroflavonol 4-reductase genes from Freesia hybrida play important and partially overlapping roles in the biosynthesis of flavonoids[J]. Frontiers in Plant Science,2017,8:428,doi:10. 3389/fpls. 2017. 00428. |
[1] | 李楠, 田小霞, 毛培春, 郑明利, 孟林, 云岚. 马蔺花器官表型特征及色素分析[J]. 植物研究, 2024, 44(1): 51-61. |
[2] | 闫惠铃, 张鑫鑫, 赵曦阳, 曲冠证, 王兆宁, 韩锐. 光信号对AmRosea1过表达84K杨花青素合成及基因表达的影响[J]. 植物研究, 2023, 43(6): 815-825. |
[3] | 杨庆华, 葛斌杰, 张大生. 唇形科兰香草蓝色花粉形成观察与成分分析[J]. 植物研究, 2023, 43(5): 794-800. |
[4] | 李娜, 王潇楠. 拟南芥种皮色素形成突变体的筛选与表型鉴定[J]. 植物研究, 2023, 43(1): 59-68. |
[5] | 赵历强, 单春苗, 张声祥, 施圆圆, 马克龙, 吴家文. 基于转录组测序的细风轮花青素合成途径及关键酶基因分析[J]. 植物研究, 2020, 40(6): 886-896. |
[6] | 李辛雷, 王佳童, 孙振元, 王洁, 殷恒福, 范正琪, 李纪元. 崇左金花茶花朵和叶片类黄酮UPLC-Q-TOF-MS分析[J]. 植物研究, 2019, 39(3): 365-371. |
[7] | 邵婉璐, 李月灵, 高松, 李钧敏, 梁宗锁. 光照强度对成熟红颜草莓果实着色和花青素生物合成的影响及可能的分子机制[J]. 植物研究, 2018, 38(5): 661-668. |
[8] | 王荣, 成梦琳, 刘慧娜, 赵大球, 陶俊. 黑暗条件下褪黑素对栀子叶片类黄酮含量及相关基因表达水平的影响[J]. 植物研究, 2018, 38(4): 559-567. |
[9] | 徐梦珂, 李丹, 孟来生, 蒋继宏. 拟南芥转录因子Ethylene-insensitive3(EIN3)抑制花青素的合成[J]. 植物研究, 2018, 38(1): 148-154. |
[10] | 徐夙侠, 黄青云, 林春松, 黄一锦, 胡欧. 三色(Bougainvillea peruviana‘Thimma’)在转录水平的甜菜色素和类黄酮积累比较[J]. 植物研究, 2017, 37(2): 249-258. |
[11] | 张丹, 任洁, 刘红梅, 王慧梅. 干旱胁迫对红松主要次生代谢产物的含量及其DPPH清除能力的影响[J]. 植物研究, 2016, 36(4): 542-548. |
[12] | 许志茹1,2,3;马静1,2,3;崔国新1,2,3;刘通1,2,3;刘关君1,2,3. 芜菁类黄酮3′-羟化酶基因的功能鉴定及启动子初步分析[J]. 植物研究, 2015, 35(4): 572-582. |
[13] | 陈清1;江雷雨1;王燕1;张云婷1;王小蓉2;汤浩茹1*. 黑莓糖基转移酶基因UGT78H2的分离鉴定及与类黄酮化合物的分子对接[J]. 植物研究, 2015, 35(2): 270-278. |
[14] | 李亚丽;胡宗利;张彬;朱明库;陈国平*. 光对紫甘蓝花青素合成代谢影响及基因表达模式分析[J]. 植物研究, 2012, 32(4): 397-401. |
[15] | 石少川;高亦珂*;张秀海;孙佳琦;赵伶俐;王叶. 植物花青素生物合成相关基因的研究及应用[J]. 植物研究, 2011, 31(5): 633-640. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||