植物研究 ›› 2023, Vol. 43 ›› Issue (6): 815-825.doi: 10.7525/j.issn.1673-5102.2023.06.004
闫惠铃1, 张鑫鑫1, 赵曦阳2, 曲冠证1, 王兆宁1, 韩锐2()
收稿日期:
2023-01-28
出版日期:
2023-11-20
发布日期:
2023-11-08
通讯作者:
韩锐
E-mail:hanrui4579@jlau.edu.cn
作者简介:
闫惠铃(1995—),女,硕士研究生,主要从事分子育种方面的研究工作。
基金资助:
Huiling YAN1, Xinxin ZHANG1, Xiyang ZHAO2, Guanzheng QU1, Zhaoning WANG1, Rui HAN2()
Received:
2023-01-28
Online:
2023-11-20
Published:
2023-11-08
Contact:
Rui HAN
E-mail:hanrui4579@jlau.edu.cn
About author:
E-mail:hanrui4579@jlau.edu.cnSupported by:
摘要:
为了探究光信号引起AmRosea1过表达84K杨(Populus alba×P.glandulosa ‘84K’)植株颜色变化的原因,以野生型和AmRosea1过表达84K杨为试验材料,开展LED光、自然光、LED红光、LED蓝光和LED红蓝光处理,测定不同光强和光质下野生型和转基因株系的生理指标变化情况。结果显示:在不同光强下,与LED光处理相比,自然光处理下转基因株系叶片变红,花青素和可溶性糖含量升高,叶绿素含量和POD活性降低。不同光质处理30 d后,与LED红光和蓝光相比,在LED红蓝光诱导下,转基因株系叶片变红,且花青素含量升高,可溶性糖含量和POD活性下降,叶绿素含量略有降低。根据试验结果推测,强光和红蓝光质能激活AmRosea1过表达84K杨的花青素生物合成通路,使转基因株系积累大量的花青素,从而使植株叶片变红。
中图分类号:
闫惠铃, 张鑫鑫, 赵曦阳, 曲冠证, 王兆宁, 韩锐. 光信号对AmRosea1过表达84K杨花青素合成及基因表达的影响[J]. 植物研究, 2023, 43(6): 815-825.
Huiling YAN, Xinxin ZHANG, Xiyang ZHAO, Guanzheng QU, Zhaoning WANG, Rui HAN. Effects of Light Signal on Anthocyanin Biosynthesis and Gene Expression of AmRosea1 Overexpressed 84K Poplar[J]. Bulletin of Botanical Research, 2023, 43(6): 815-825.
表1
qRT-PCR引物序列
基因名 Gene | 上游引物(5′→3′) Upstream primer(5′→3′) | 下游引物(5’→3’) Downstream primer(5’→3’) |
---|---|---|
AmRosea1 | AGAGTATGGTGAAGGGAAATGG | CCGACCTCTTTTGATATTTGGC |
IF7MAT | CAATCACTTTCCCATGCACTC | TCACCATTACACTCGGCAAG |
FG2 | GTCACTCAGGGTTTAGCTCG | CCTATTTATCTCCACCCCAGC |
DFR | GGAAACATGCAGAGAAAAGGG | GGAATAAGAGGACCGTGGAAC |
ANS | CCAGTAGAGGTGAAGCAAGAG | GACTTGCTCCTTTCTCCACTC |
BZ1.1 | GACAGGGAGAAAGGTGAGTTG | CATGAGCAGAAAGTGAGGTAGG |
Actin | AATACCCCATTGAGCACGG | ACTCACACCATCACCAGAAT |
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