植物研究 ›› 2016, Vol. 36 ›› Issue (4): 556-564.doi: 10.7525/j.issn.1673-5102.2016.04.011
叶思诚, 姚小华, 王开良, 林萍, 龚洪恩, 卓仁英
收稿日期:
2016-03-16
出版日期:
2016-07-15
发布日期:
2016-06-15
通讯作者:
姚小华,E-mail:yaoxh168@163.com
E-mail:yaoxh168@163.com
作者简介:
叶思诚(1985-),男,博士研究生,主要从事经济林栽培育种研究。
基金资助:
YE Si-Cheng, YAO Xiao-Hua, WANG Kai-Liang, LIN Ping, GONG Hong-En, ZHUO Ren-Ying
Received:
2016-03-16
Online:
2016-07-15
Published:
2016-06-15
Supported by:
摘要: 采用RT-PCR技术从油茶中分离出一个柠檬酸合成酶基因,该基因的cDNA全长1416 bp,编码471个氨基酸,推导的蛋白分子量为52.74 kD,理论等电点(PI)为6.95。同源比对显示其与其他植物的CS蛋白序列高度同源,将该基因命名为CoCS(Gen Bank登录号:KU161147)。系统进化树分析表明油茶CoCS与杜鹃和葡萄的CS蛋白的亲缘关系较近。荧光定量PCR分析结果表明,油茶受到低磷胁迫后根系CoCS基因的表达受到低磷诱导,表达量呈现先升高后降低的趋势;不同油茶品种不同组织(根、茎、叶)中的CoCS基因在不用磷处理下的表达模式不同。
中图分类号:
叶思诚, 姚小华, 王开良, 林萍, 龚洪恩, 卓仁英. 油茶柠檬酸合成酶(CS)基因的克隆和表达分析[J]. 植物研究, 2016, 36(4): 556-564.
YE Si-Cheng, YAO Xiao-Hua, WANG Kai-Liang, LIN Ping, GONG Hong-En, ZHUO Ren-Ying. Cloning and Expression Analysis of Citrate Synthase(CS) Gene in Camellia oleifera[J]. Bulletin of Botanical Research, 2016, 36(4): 556-564.
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