植物研究 ›› 2023, Vol. 43 ›› Issue (4): 601-611.doi: 10.7525/j.issn.1673-5102.2023.04.013
张昊楠1,2, 陈珊珊2,3, 徐建民2, 罗萍2, 王晓萍2, 许志茹1, 范春节2,3()
收稿日期:
2022-07-13
出版日期:
2023-07-20
发布日期:
2023-06-30
通讯作者:
范春节
E-mail:fanchunjie@caf.ac.cn
作者简介:
张昊楠(1998—),女,硕士研究生,主要从事生物医药与发育生物学方向研究。
基金资助:
Haonan ZHANG1,2, Shanshan CHEN2,3, Jianmin XU2, Ping LUO2, Xiaoping WANG2, Zhiru XU1, Chunjie FAN2,3()
Received:
2022-07-13
Online:
2023-07-20
Published:
2023-06-30
Contact:
Chunjie FAN
E-mail:fanchunjie@caf.ac.cn
About author:
E-mail: fanchunjie@caf.ac.cnSupported by:
摘要:
为探究WALLS ARE THIN (WAT1)在木本植物中木材形成以及响应胁迫中的作用,利用生物信息学工具进行分析,并以巨桉(Eucalyptus grandis)为材料克隆EgrWAT1S及其另一转录本EgrWAT1L,通过实时荧光定量PCR(qRT-PCR)探究其在不同组织、节间以及响应胁迫时的表达模式。结果表明:EgrWAT1S在韧皮部表达量较高,而EgrWAT1L主要表达在根部。在茉莉酸甲酯(MeJA)、水杨酸(SA)处理和盐胁迫以及缺磷、缺硼处理时,其表达存在着明显不同的模式,在MeJA、SA处理时甚至存在着相反的表达模式。这些结果表明EgrWAT1L基因可能通过转录调控来影响EgrWAT1S表达和进一步的蛋白翻译来响应激素和胁迫处理。为进一步研究WAT1基因在巨桉生长发育过程中的作用和调控方式提供基础,也为将来桉树的分子育种 提供可能。
中图分类号:
张昊楠, 陈珊珊, 徐建民, 罗萍, 王晓萍, 许志茹, 范春节. 巨桉EgrWAT1基因克隆和功能初步分析[J]. 植物研究, 2023, 43(4): 601-611.
Haonan ZHANG, Shanshan CHEN, Jianmin XU, Ping LUO, Xiaoping WANG, Zhiru XU, Chunjie FAN. Cloning and Functional Analysis of EgrWAT1 Gene in Eucalyptus grandis[J]. Bulletin of Botanical Research, 2023, 43(4): 601-611.
表1
引物序列
引物名称 Primer name | 引物序列(5′→3′) Primer sequences(5′→3′) |
---|---|
EgrWAT1 PCR F | AGGGCTTGCTCTGTGTTCCTG |
EgrWAT1 PCR R | CATCCTGAGGATGATCTACGGTC |
EgrWAT1S qRT-PCR F | CTCGGGATGGAGAAACTCAG |
EgrWAT1S qRT-PCR R | CTTTTCCATGCGTCAAGACA |
EgrWAT1L qRT-PCR F | TTCCCGATACTGTACTAGATCACC |
EgrWAT1L qRT-PCR R | TCCATGCGTCAAGACATCAG |
EgrEF2-F | TCCAATCCGAGTCGCTGTCATTGT |
EgrEF2-R | TGATGAGCCTCTCTGGTTTGACCT |
EgrWAT1 pROKII F | GCTCTAGAATGGAAAACGGCGCTTC |
EgrWAT1 pROKII R | GGGGTACCCTACTCGACAGTAGTCACTG |
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