Expression Profiles of Arabidopsis thaliana VQ Gene Family in DefenseRelated Hormones Treatments

doi:10.3724/SP.J.1143.2012.12032

Abstract

Abstract:

Several VQ proteins are recently identified as WRKY factorsinteracting partners in Arabidopsis and involved in regulating physiological processes. Searching of genomic databases found that VQ gene family is specific to land plants and these VQ genes encode proteins characteristic of a conserved VQmotif. It consists of 34 representatives in Arabidopsis and can be divided into two groups based on the similarity of the amino acid sequences. To understand the functions of Arabidopsis VQ proteins, we examined the expression profiles of AtVQ genes in various defenserelated hormones treatments. qRTPCR analysis revealed that a majority of them were differently regulated in response to salicylic acid (SA), methyl jasmonate (MeJA), or 1aminocyclopropane1carboxylate (ACC). And some members are induced by two of these three hormones. Moreover, four members (AtVQ3, AtVQ18, AtVQ23 and AtVQ24) are induced by SA, MeJA and ACC simultaneity. However, there is only one AtVQ gene (AtVQ27) is upregulated after spraying of abscisic acid (ABA). These results suggest that Arabidopsis VQ genes may be involved in plant defense responses.

Key words: Arabidopsis thaliana, VQ gene family, ABA, SA, JA, ET

摘要：

近年来与WRKY转录因子相互作用的VQmotif蛋白逐渐引起广泛关注。它是一类植物特异性蛋白，目前在拟南芥（Arabidopsis thaliana）中已鉴定到34个成员，据其所编码氨基酸的序列相似性构建系统进化树，这34个成员聚集成两大簇。实时定量荧光PCR分析表明在SA（水杨酸），MeJA（茉莉酸甲酯），ACC（乙烯前体），ABA（脱落酸）处理下AtVQ基因家族中有多个成员分别受SA，JA，ET高倍诱导，部分成员受SA，JA，ET中两种激素诱导，其中AtVQ3，AtVQ18，AtVQ23和 AtVQ24同时受SA，JA，ET诱导，仅AtVQ27受ABA诱导，推测拟南芥VQ家族成员在抗病方面起作用。

关键词: 拟南芥, VQ 基因家族, 水杨酸, 茉莉酸甲酯, 乙烯, 脱落酸

CLC Number:

Q 945

LIN Yuan-Yuan- , YU Di-Qiu. Expression Profiles of Arabidopsis thaliana VQ Gene Family in DefenseRelated Hormones Treatments[J]. Plant Diversity, 2012, 34(5): 509-518.

林渊源1、2 , 余迪求1. 拟南芥VQ基因家族响应抗性相关激素表达谱分析[J]. Plant Diversity, 2012, 34(5): 509-518.

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