Xylanins A-P, sixteen new guaiane-type dimers from the branches and leaves of Xylopia vielana with anti-proliferative activity against PANC-1 cell line

doi:10.1007/s13659-025-00574-z

Natural Products and Bioprospecting ›› 2026, Vol. 16 ›› Issue (2): 19-19.DOI: 10.1007/s13659-025-00574-z

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Xylanins A-P, sixteen new guaiane-type dimers from the branches and leaves of Xylopia vielana with anti-proliferative activity against PANC-1 cell line

Xianglian Jiang, Ting Zhang, Fancheng Meng, Min Chen, Guowei Wang

College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, People's Republic of China

Received:2025-09-07 Online:2026-04-22 Published:2026-04-22
Contact: Guowei Wang,Email:wangguowei@swu.edu.cn
Supported by:
This work was sponsored by the National Natural Science Foundation of China (Grant Number 82073724), Sub-project of Joint Funds of National Natural Science Foundation of China (Grant Number U20A20401), and Special Fund for Youth Team of Southwest University (Grant Number SWU—XJLJ202306).

Xylanins A-P, sixteen new guaiane-type dimers from the branches and leaves of Xylopia vielana with anti-proliferative activity against PANC-1 cell line

Xianglian Jiang, Ting Zhang, Fancheng Meng, Min Chen, Guowei Wang

College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, People's Republic of China

通讯作者: Guowei Wang,Email:wangguowei@swu.edu.cn
基金资助:
This work was sponsored by the National Natural Science Foundation of China (Grant Number 82073724), Sub-project of Joint Funds of National Natural Science Foundation of China (Grant Number U20A20401), and Special Fund for Youth Team of Southwest University (Grant Number SWU—XJLJ202306).

Abstract

Abstract: Sixteen previously undiscovered guaiane-type sesquiterpene dimers, xylanins A-P (1-16), along with six known analogues (17-22), were isolated from the branches and leaves of Xylopia vielana with chromatographic techniques. Their structures including absolute configurations were determined by high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), nuclear magnetic resonance (NMR) data, electron circular dichroism (ECD) spectra, as well as X-ray crystallographic analysis. In cytotoxicity test, we found that five compounds (6, 7, 8, 9 and 12) had cytotoxic activities in vitro against the human pancreatic cancer (PANC-1) and human prostate cancer (PC-3) cell lines. Of these compounds, compound 8 showed a relatively greater cytotoxic effect against PANC-1 cell lines with half maximal inhibitory concentration (IC₅₀) value of 1.06 μM. Flow cytometry analysis showed that the apoptosis rate of PANC-1 cells increased with increasing concentrations of compound 8, and also demonstrated that the cell cycle of PANC-1 cells was arrested at S phase by the treatment of compound 8. By the invasion test, compound 8 was found to restrain the invasion of PANC-1 cells. In autophagy assay, we observed increased microtubule-associated protein 1 light chain 3 (LC3) by immunofluorescence in the compound 8-treated group.

Key words: Structural identification, Sesquiterpenes, Xylopia vielana branches and leaves, Anti-proliferative activity

摘要： Sixteen previously undiscovered guaiane-type sesquiterpene dimers, xylanins A-P (1-16), along with six known analogues (17-22), were isolated from the branches and leaves of Xylopia vielana with chromatographic techniques. Their structures including absolute configurations were determined by high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), nuclear magnetic resonance (NMR) data, electron circular dichroism (ECD) spectra, as well as X-ray crystallographic analysis. In cytotoxicity test, we found that five compounds (6, 7, 8, 9 and 12) had cytotoxic activities in vitro against the human pancreatic cancer (PANC-1) and human prostate cancer (PC-3) cell lines. Of these compounds, compound 8 showed a relatively greater cytotoxic effect against PANC-1 cell lines with half maximal inhibitory concentration (IC₅₀) value of 1.06 μM. Flow cytometry analysis showed that the apoptosis rate of PANC-1 cells increased with increasing concentrations of compound 8, and also demonstrated that the cell cycle of PANC-1 cells was arrested at S phase by the treatment of compound 8. By the invasion test, compound 8 was found to restrain the invasion of PANC-1 cells. In autophagy assay, we observed increased microtubule-associated protein 1 light chain 3 (LC3) by immunofluorescence in the compound 8-treated group.

关键词: Structural identification, Sesquiterpenes, Xylopia vielana branches and leaves, Anti-proliferative activity

Xianglian Jiang, Ting Zhang, Fancheng Meng, Min Chen, Guowei Wang. Xylanins A-P, sixteen new guaiane-type dimers from the branches and leaves of Xylopia vielana with anti-proliferative activity against PANC-1 cell line[J]. Natural Products and Bioprospecting, 2026, 16(2): 19-19.

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Xylanins A-P, sixteen new guaiane-type dimers from the branches and leaves of Xylopia vielana with anti-proliferative activity against PANC-1 cell line

Xylanins A-P, sixteen new guaiane-type dimers from the branches and leaves of Xylopia vielana with anti-proliferative activity against PANC-1 cell line

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