Abstract: This study evaluated toxic efficacy of Eupatorium adenophorum extracts, against the Kunming mice. In acute study, we firstly tested median lethal dose(LD₅₀) in mice of three cadinene sesquiterpenes 2-deoxo-2-(acetyloxy)-9-oxoageraphorone(DAOA), 9-oxo-agerophorone(OA) and 9-oxo-10, 11-dehydro-agerophorone(ODA) from Eupatorium adenophorum(Ea). DAOA(215-4640 mg/kg BW, given orally) showed lowest LD₅₀ at 926 mg/kg BW for male mice in contrast with OA(1470 mg/kg BW) and ODA(1470 mg/kg BW). In sub-acute study, repeated doses(75-300 mg/kg BW, for 7 days) of DAOA/OA increased blood parameters, liver and spleen index in dose dependent relationship, along with decrease in thymus index. The blood biochemical and histopathological examination showed that DAOA/OA dose 300 mg/kg BW significantly causes pathological changes of hepatic lobules and hepatocytes, which are consistent with cholestasis and hepatic injury. 75 mg/kg dose of DAOA/OA was found to be approximately/totally safe over the span of 7 days treatment showing no change in all above described parameters. Cadinene sesquiterpenes guarantee low risk to environment as a type of low toxic botanical components, which may find potential application in biopesticides development field.

Key words: Eupatorium adenophorum, Sesquiterpenes, Histopathology, Environmental toxicity, Biopesticide

摘要： This study evaluated toxic efficacy of Eupatorium adenophorum extracts, against the Kunming mice. In acute study, we firstly tested median lethal dose(LD₅₀) in mice of three cadinene sesquiterpenes 2-deoxo-2-(acetyloxy)-9-oxoageraphorone(DAOA), 9-oxo-agerophorone(OA) and 9-oxo-10, 11-dehydro-agerophorone(ODA) from Eupatorium adenophorum(Ea). DAOA(215-4640 mg/kg BW, given orally) showed lowest LD₅₀ at 926 mg/kg BW for male mice in contrast with OA(1470 mg/kg BW) and ODA(1470 mg/kg BW). In sub-acute study, repeated doses(75-300 mg/kg BW, for 7 days) of DAOA/OA increased blood parameters, liver and spleen index in dose dependent relationship, along with decrease in thymus index. The blood biochemical and histopathological examination showed that DAOA/OA dose 300 mg/kg BW significantly causes pathological changes of hepatic lobules and hepatocytes, which are consistent with cholestasis and hepatic injury. 75 mg/kg dose of DAOA/OA was found to be approximately/totally safe over the span of 7 days treatment showing no change in all above described parameters. Cadinene sesquiterpenes guarantee low risk to environment as a type of low toxic botanical components, which may find potential application in biopesticides development field.

关键词: Eupatorium adenophorum, Sesquiterpenes, Histopathology, Environmental toxicity, Biopesticide