In Vitro Antioxidant and Cytotoxic Activities of 18 Plants from the Erkowit Region, Eastern Sudan

doi:10.1007/s13659-018-0155-0

Natural Products and Bioprospecting ›› 2018, Vol. 8 ›› Issue (2): 97-105.DOI: 10.1007/s13659-018-0155-0

• ORIGINAL ARTICLES • Previous Articles Next Articles

In Vitro Antioxidant and Cytotoxic Activities of 18 Plants from the Erkowit Region, Eastern Sudan

Manar Adam¹, Gihan O. M. Elhassan¹, Sakina Yagi¹, Fatma Sezer Senol², Ilkay Erdogan Orhan², Abdel Azim Ahmed¹, Thomas Efferth³

1 Department of Botany, Faculty of Science, University of Khartoum, Khartoum, Sudan;
2 Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey;
3 Department of Pharmaceutical Biology, Johannes Gutenberg University, 55128 Mainz, Germany

Received:2018-01-03 Revised:2018-02-09 Online:2018-04-26 Published:2018-04-24
Contact: Thomas Efferth
Supported by:
The authors would like to acknowledge Prof. Maha Kordofani (Botany Department, Faculty of Science, University of Khartoum) for the identification of the plants. We are grateful to a stipend of the German Academic Exchange Service (DAAD) to Gihan Elhaboob.

In Vitro Antioxidant and Cytotoxic Activities of 18 Plants from the Erkowit Region, Eastern Sudan

Manar Adam¹, Gihan O. M. Elhassan¹, Sakina Yagi¹, Fatma Sezer Senol², Ilkay Erdogan Orhan², Abdel Azim Ahmed¹, Thomas Efferth³

1 Department of Botany, Faculty of Science, University of Khartoum, Khartoum, Sudan;
2 Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey;
3 Department of Pharmaceutical Biology, Johannes Gutenberg University, 55128 Mainz, Germany

通讯作者: Thomas Efferth
基金资助:
The authors would like to acknowledge Prof. Maha Kordofani (Botany Department, Faculty of Science, University of Khartoum) for the identification of the plants. We are grateful to a stipend of the German Academic Exchange Service (DAAD) to Gihan Elhaboob.

Abstract

Abstract: We investigated the antioxidant potential and cytotoxicity towards human CCRF-CEM leukemia cells of 57 extracts obtained from 18 plants collected in the Erkowit region, eastern Sudan. The antioxidant activity was determined by measuring the radical scavenging effects against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and N,N-dimethyl-p-phenylendiamine (DMPD), metal-chelation capacity, ferric-reducing (FRAP) and phosphomolibdenum-reducing antioxidant power (PRAP) methods using ELISA microtiter assays. Total phenol and flavonoid amounts of the extracts were determined spectrophotometrically. Cytotoxicity towards CCRF-CEM cells was evaluated by the resazurin reduction assay. Geranium favosum followed by Kalanchoe glaucescens, Malva parviflora, Aizoon canariense, and Coleus barbatus, respectively, possessed the highest antioxidant activity among the studied plants. Chrozophora oblongifolia and K. glaucescens exerted considerable cytotoxicity against CCRF-CEM leukemia cells. These plants may serve as source for the further development of natural antioxidant and antitumor agents.

Key words: Antioxidant activity, Cytotoxicity, Pharmacognosy, Plant extract

摘要： We investigated the antioxidant potential and cytotoxicity towards human CCRF-CEM leukemia cells of 57 extracts obtained from 18 plants collected in the Erkowit region, eastern Sudan. The antioxidant activity was determined by measuring the radical scavenging effects against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and N,N-dimethyl-p-phenylendiamine (DMPD), metal-chelation capacity, ferric-reducing (FRAP) and phosphomolibdenum-reducing antioxidant power (PRAP) methods using ELISA microtiter assays. Total phenol and flavonoid amounts of the extracts were determined spectrophotometrically. Cytotoxicity towards CCRF-CEM cells was evaluated by the resazurin reduction assay. Geranium favosum followed by Kalanchoe glaucescens, Malva parviflora, Aizoon canariense, and Coleus barbatus, respectively, possessed the highest antioxidant activity among the studied plants. Chrozophora oblongifolia and K. glaucescens exerted considerable cytotoxicity against CCRF-CEM leukemia cells. These plants may serve as source for the further development of natural antioxidant and antitumor agents.

关键词: Antioxidant activity, Cytotoxicity, Pharmacognosy, Plant extract

Manar Adam, Gihan O. M. Elhassan, Sakina Yagi, Fatma Sezer Senol, Ilkay Erdogan Orhan, Abdel Azim Ahmed, Thomas Efferth. In Vitro Antioxidant and Cytotoxic Activities of 18 Plants from the Erkowit Region, Eastern Sudan[J]. Natural Products and Bioprospecting, 2018, 8(2): 97-105.

References

1. H. Khalid, W.E. Abdalla, H. Abdelgadir, T. Opatz, T. Efferth, Nat. Prod. Bioprospect. 2, 92-103 (2012)
2. U.P. Albuquerque, P.M. de Medeiros, M.A. Ramos, W.S.F. Júnior, A.L.P. Nascimento, W.M.T. Avilez, G.J. de Melo, Revist. Brasil. Farmacogn. 24, 110-115 (2014)
3. I.K. Khafagi, A. Dewedar, J. Ethnopharmacol. 71, 365-376 (2000)
4. C. Gyllenhaal, M.R. Kadushin, B. Southavong, K. Sydara, S. Bouamanivong, M. Xaiveu, L.T. Xuan, N.T. Hiep, N.V. Hung, P.K. Loc, L.X. Dac, T.Q. Bich, N.M. Cuong, H.M. Ly, H.J. Zhang, S.G. Franzblau, H. Xie, M.C. Riley, B.G. Elkington, H.T. Nguyen, D.P. Waller, C.Y. Ma, P. Tamez, G.T. Tan, J.M. Pezzuto, D.D. Soejarto, Pharm. Biol. 50, 30-41 (2012)
5. P. Rajendran, N. Nandakumar, T. Rengarajan, R. Palaniswami, E.N. Gnanadhas, U. Lakshminarasaiah, J. Gopas, I. Nishigaki, Clin. Chim. Acta 436, 332-347 (2014)
6. T.L. Sampaio, R.R. Menezes, M.F. da Costa, G.C. Meneses, M.C. Arrieta, A.J.C. Filho, G.B. de Morais, A.B. Libório, R.S. Alves, J.S. Evangelista, A.M. Martins, Phytomedicine 23, 1843-1852 (2016)
7. F.S. Youssef, M.L. Ashour, M. Sobeh, H.A. El-Beshbishy, A.N. Singab, M. Wink, Phytomedicine 23, 1484-1493 (2016)
8. K. Suchal, S. Malik, N. Gamad, R.K. Malhotra, S.N. Goyal, J. Bhatia, D.S. Arya, Phytomedicine 23, 1401-1408 (2016)
9. L. Subedi, B.P. Gaire, M.H. Do, T.H. Lee, S.Y. Kim, Phytomedicine 23, 872-881 (2016)
10. R. Simeonova, V. Vitcheva, I. Krasteva, P. Zdraveva, S. Konstantinov, I. Ionkova, Phytomedicine 23, 483-490 (2016)
11. J. Ferlay, H.R. Shin, F. Bray, D. Forman, C. Mathers, D.M. Parkin, Int. J. Cancer 127, 2893-2917 (2010)
12. IARC, Cancer Incidence and Mortality Worldwide, vol. 10 (IARC Cancer Base, 2011)
13. I.L. Hsin, S.C. Wang, J.R. Li, T.C. Ciou, C.H. Wu, H.M. Wu, J.L. Ko, Phytomedicine 23, 1566-1573 (2016)
14. S. Umsumarng, P. Pitchakarn, S. Yodkeeree, W. Punfa, S. Mapoung, R.A. Ramli, S.G. Pyne, P. Limtrakul, Phytomedicine 34, 182-190 (2017)
15. E. Juengel, S. Euler, S. Maxeiner, J. Rutz, S. Justin, F. Roos, W. Khoder, K. Nelson, W.O. Bechstein, R.A. Blaheta, Phytomedicine 27, 1-7 (2017)
16. F. White, in The Vegetation of Africa:a Descriptive Memoir to Accompany the Unesco/AETFAT/UNSO Vegetation Map of Africa, Natural Resource Res, vol. 20 (UNESCO, Paris, 1983)
17. B.M. Hybertson, B. Gao, S.K. Bose, J.M. McCord, Mol. Aspects Med. 32, 234 (2011)
18. R.L. Prior, X.L. Wu, K. Schaich, J. Agricult, Food Chem. 53, 4290-4302 (2005)
19. M. Ismail, M. Ibrar, Z. Iqbal, J. Hussain, H. Hussain, M. Ahmed, A. Ejaz, M.I. Choudhary, Rec. Nat. Prod. 3, 193-197 (2009)
20. O.A.J. Afolayan, M. Aboyade, M.O. Sofidiya, J. Biol. Sci. 8, 945-949 (2008)
21. A.A. Al-Laith, J. Alkhuzai, A. Freije, Arab. J. Chem. (2015). https://doi.org/10.1016/j.arabjc.2015.03.004
22. J.S. Mossa, M.A. Al-Yahya, I.A. Al-Meshal, Fitoterapia 64, 147-158 (1993)
23. F.S. Al-Saleh, H.H. Ali, M. Mirza, Fitoterapia 64, 251-256 (1993)
24. V. Shah, S.V. Bhat, B.S. Bajwa, H. Dornauer, N.J. de Souza, Planta Med. 39, 183-185 (1980)
25. B. Gabetta, G. Zini, B. Danieli, Phytochemistry 28, 859-862 (1980)
26. A. Karagöz, N. Doruöz, Z. Zeybek, A. Aslan, J. Med. Plants Res. 3, 1034-1039 (2009)
27. O.T. Cetin, M. Akarsu, E. Burunkaya, O. Kesmez, E. Arpac, H. Cetin, Egypt J. Biol. Pest Contr. 23, 287-290 (2013)
28. R. Milad, S. El-Ahmady, A. Singab, Eur. J. Med. Plants 4, 86-104 (2014)
29. J. Boik, Natural Compounds in Cancer Therapy (Oregon Medical Press, Princeton, 2001)
30. L.K. Omosa, J.O. Midiwo, V.A. Masila, B.M. Gisacho, R. Munayi, F. Kamakama, K.B. Chemutai, G. Elhaboob, M.E.M. Saeed, S. Hamdoun, V. Kuete, T. Efferth, J. Ethnopharmacol. 179, 177-196 (2016)
31. V. Kuete, A.T. Mbaveng, E.C. Nono, C.C. Simo, M. Zeino, A.E. Nkengfack, T. Efferth, Phytomedicine 23, 856-863 (2016)
32. V. Kuete, A.T. Mbaveng, L.P. Sandjo, M. Zeino, T. Efferth, Phytomedicine 33, 62-68 (2017)
33. S. Roenqsumran, K. Musikul, A. Petsom, T. Vilaivan, P. Sanqvanich, S. Pornpakakul, S. Puthong, C. Chaichantipyuth, N. Jaiboon, N. Chaichit, Planta Med. 68, 274-277 (2002)
34. P. Wu, Y. Hsu, T. Wu, K.F. Bastow, K. Lee, Org. Lett. 8, 5207-5210 (2006)
35. U. Supratman, T. Fujita, K. Akiyama, H. Hayashi, A. Murakami, H. Sakai, K. Koshimizu, H. Ohigashi, Biosci. Biotechnol. Biochem. 5, 947-949 (2001)
36. L.S. Aisyah, Y.F. Yun, A.A. Abdilla, T. Herlina, E. Julaeha, U. Supratman, Akta Kimindo 1, 1-4 (2016)
37. D. Slamenova, E. Horvathova, Neoplasma 60, 343-354 (2013)
38. R. Dianita, I. Jantan, Pharm. Biol. 55, 1715-1739 (2017)
39. R. Schuster, L. Zeindl, W. Holzer, N. Khumpirapang, S. Okonogi, H. Viernstein, M. Mueller, Phytomedicine 24, 157-165 (2017)
40. K. Vijayaraghavan, J. Rajkumar, S.N. Bukhari, B. Al-Sayed, M.A. Seyed, Mol. Med. Rep. 15, 1007-1016 (2017)
41. I.B. Abubakar, K.H. Lim, T.S. Kam, H.S. Loh, Phytomedicine 30, 74-84 (2017)
42. W.W. Chao, C.C. Su, H.Y. Peng, S.T. Chou, Phytomedicine 34, 191-201 (2017)
43. M.S. Blois, Nature 181, 1199-1200 (1958)
44. K. Schlesier, M. Harvat, V. Bohm, R. Bitsch, Free Radic. Res. 36, 177-187 (2002)
45. M.T. Chua, Y.T. Tung, S.T. Chang, Bioresour. Technol. 99, 1918-1925 (2008)
46. M. Oyaizu, Jpn. J. Nutr. Diet. 44, 307-315 (1986)
47. G. Falcioni, D. Fedeli, L. Tiano, I. Calzuola, L. Mancinelli, V. Marsili, G. Gianfranceschi, J. Food Sci. 67, 2918-2922 (2002)
48. V. Kuete, A.T. Mbaveng, E.C. Nono, C.C. Simo, M. Zeino, A.E. Nkengfack, T. Efferth, Phytomedicine 23, 856-863 (2016)
49. V. Kuete, A.T. Mbaveng, L.P. Sandjo, M. Zeino, T. Efferth, Phytomedicine 33, 62-68 (2017)
50. V.L. Singleton, J.A. Rossi Jr., Am. J. Enol. Vitic. 16, 144-158 (1965)
51. R. Woisky, A. Salatino, J. Agric. Res. 37, 99-105 (1998)
52. M. Braun, H. Burgstaller, A.M. Hamdoun, H. Walter, Common weeds of central Sudan (Verlag Josef Margraf. Germany, 1991)
53. M.E.M. Saeed, H. Abdelgadir, Y. Sugimoto, H.E. Khalid, T. Efferth, J. Ethnopharmacol. 174, 644-658 (2015)
54. G.E.B. El Ghazali, M.S. El-Tohami, A.A.B. El-Egami, Medicinal plants of the Sudan. Part Ⅲ. Medicinal plants of the White Nile provinces (Khartoum University Press, Khartoum, Sudan, 1994)
55. M.A. Ahmed, A.A. Mariod, I.H. Hussein, A. Kamal-eldin, Int. J. Pharm. Rev. Res. 4, 227-232 (2014)
56. G.E.B. El Ghazali, M.S. El Tohami, A.A. El Egami, W.E. Abdalla, M. Galal, Medicinal Plants of the Sudan Part IV. Medicinal Plants of North Kordofan (Omdurman Islamic University Press, Khartoum, Sudan, 1997)

[1]	Pengkun Li, Qin Li, Aimin Fu, Yang Xiao, Chunmei Chen, Hucheng Zhu, Changxing Qi, Wei Wei, Yuan Zhou, Yonghui Zhang. Emestrin-type epipolythiodioxopiperazines from Aspergillus nidulans with cytotoxic activities by regulating PI3K/AKT and mitochondrial apoptotic pathways [J]. Natural Products and Bioprospecting, 2025, 15(2): 17-17.
[2]	Dong-Yang Wang, Ming-Xing Li, Yan-Chao Xu, Peng Fu, Wei-Ming Zhu, Li-Ping Wang. Dibohemamines I-O from Streptomyces sp. GZWMJZ-662, an endophytic actinomycete from the medicinal and edible plant Houttuynia cordata Thunb. [J]. Natural Products and Bioprospecting, 2025, 15(1): 9-9.
[3]	Yue-Mei Chen, Nan-Kai Cao, Si-Si Zhu, Meng Ding, Hai-Zhen Liang, Ming-Bo Zhao, Ke-Wu Zeng, Peng-Fei Tu, Yong Jiang. Euchrestifolines A-O, fifteen novel carbazole alkaloids with potent anti-ferroptotic activity from Murraya euchrestifolia [J]. Natural Products and Bioprospecting, 2025, 15(1): 5-5.
[4]	Alica Fischle, Mika Lutsch, Florian Hübner, Linda Sch?ker-Hübner, Lina Schürmann, Finn K. Hansen, Svetlana A. Kalinina. Micro-scale screening of genetically modified Fusarium fujikuroi strain extends the apicidin family [J]. Natural Products and Bioprospecting, 2024, 14(6): 51-51.
[5]	Qi-Xiu Hai, Kun Hu, Su-Ping Chen, Yang-Yang Fu, Xiao-Nian Li, Han-Dong Sun, Hong-Ping He, Pema-Tenzin Puno. Silvaticusins A-D: ent-kaurane diterpenoids and a cyclobutane-containing ent-kaurane dimer from Isodon silvaticus [J]. Natural Products and Bioprospecting, 2024, 14(5): 45-45.
[6]	Rudra Narayan Subudhi, Neelam Poonia, Dilpreet Singh, Vimal Arora. Natural approaches for the management of ulcerative colitis: evidence of preclinical and clinical investigations [J]. Natural Products and Bioprospecting, 2024, 14(5): 42-42.
[7]	Srijan Banerjee, Gustavo Cabrera-Barjas, Jaime Tapia, João Paulo Fabi, Cedric Delattre, Aparna Banerjee. Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additive [J]. Natural Products and Bioprospecting, 2024, 14(2): 3-3.
[8]	Teresa S. Catalá, Linn G. Speidel, Arlette Wenzel-Storjohann, Thorsten Dittmar, Deniz Tasdemir. Bioactivity profile of dissolved organic matter and its relation to molecular composition [J]. Natural Products and Bioprospecting, 2023, 13(5): 32-32.
[9]	Shuyuan Mo, Ziming Zhao, Zi Ye, Zhihong Huang, Yaxin Zhang, Wanqi Yang, Jianping Wang, Zhengxi Hu, Yonghui Zhang. New secondary metabolites with cytotoxicity from fungus Penicillium roqueforti [J]. Natural Products and Bioprospecting, 2023, 13(3): 17-17.
[10]	Li Hou, Cui-Xuan Mei, Chun-Mao Yuan, Gui-Hua Tang, Duo-Zhi Chen, Qing Zhao, Hong-Ping He, Ming-Ming Cao, Xiao-Jiang Hao. Five new limonoids isolated from Walsura robusta [J]. Natural Products and Bioprospecting, 2023, 13(2): 7-7.
[11]	Ya-Li Hu, Xing-Ren Li, Gang Xu. Carascynol A, a hybrid of caryophyllane-type terpenoid and a C₆ unit degraded by polyprenylated acylphloroglucinols from Hypericum ascyron [J]. Natural Products and Bioprospecting, 2022, 12(6): 38-38.
[12]	Xin Zhang, Yun-Bao Ma, Xiao-Feng He, Tian-Ze Li, Chang-An Geng, Li-Hua Su, Shuang Tang, Zhen Gao, Ji-Jun Chen. Artemyrianosins A–J, cytotoxic germacrane-type sesquiterpene lactones from Artemisia myriantha [J]. Natural Products and Bioprospecting, 2022, 12(3): 16-16.
[13]	Natividad Herrera Cano, Sebastian A. Andujar, Cristina Theoduloz, Daniel A. Wunderlin, Ana N. Santiago, Guillermo Schmeda-Hirschmann, Ricardo D. Enriz, Gabriela E. Feresin. Arylated analogues of cypronazole: fungicidal effect and activity on human fibroblasts. Docking analysis and molecular dynamics simulations [J]. Natural Products and Bioprospecting, 2022, 12(2): 9-9.
[14]	Ruo-Song Zhang, Yang-Yang Liu, Pei-Feng Zhu, Qiong Jin, Zhi Dai, Xiao-Dong Luo. Furostanol Saponins from Asparagus cochinchinensis and Their Cytotoxicity [J]. Natural Products and Bioprospecting, 2021, 11(6): 651-658.
[15]	Patrick O. Sakyi, Richard K. Amewu, Robert N. O. A. Devine, Emahi Ismaila, Whelton A. Miller, Samuel K. Kwofie. The Search for Putative Hits in Combating Leishmaniasis: The Contributions of Natural Products Over the Last Decade [J]. Natural Products and Bioprospecting, 2021, 11(5): 489-544.

In Vitro Antioxidant and Cytotoxic Activities of 18 Plants from the Erkowit Region, Eastern Sudan

In Vitro Antioxidant and Cytotoxic Activities of 18 Plants from the Erkowit Region, Eastern Sudan

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments