Essential oil extracted from Quzhou Aurantii Fructus prevents acute liver failure through inhibiting lipopolysaccharide-mediated inflammatory response

doi:10.1007/s13659-023-00398-9

应用天然产物 ›› 2023, Vol. 13 ›› Issue (5): 36-36.DOI: 10.1007/s13659-023-00398-9

Essential oil extracted from Quzhou Aurantii Fructus prevents acute liver failure through inhibiting lipopolysaccharide-mediated inflammatory response

Tian Lan¹, Wen Wang², De-Lian Huang³, Xi-Xi Zeng¹, Xiao-Xiao Wang⁴, Jian Wang⁴, Yu-Hua Tong^1,5, Zhu-Jun Mao^5,6, Si-Wei Wang^1,5

1. The Joint Innovation Center for Health and Medicine, Quzhou People's Hospital, The Quzhou Affiliated Hospital of Wenzhou Medical University, No. 100 Minjiang Road, Quzhou, 324000, China;
2. Preventive Treatment Center, Zhejiang Chinese Medical University Affiliated Four-provinces Marginal Hospital of Traditional Chinese Medicine, Quzhou Hospital of Traditional Chinese Medicine, Quzhou, 324000, China;
3. School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou, 310053, China;
4. Department of Drug Analysis Center, Quzhou Institute for Food and Drug Control, Quzhou, 324000, China;
5. Department of Ophthalmology, Quzhou People's Hospital, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou, 324000, China;
6. College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, No. 548 Binwen Road, Hangzhou, 310053, China

收稿日期:2023-07-08 出版日期:2023-10-24 发布日期:2023-11-03
通讯作者: Yu-Hua Tong,E-mail:yuhuatong@126.com;Zhu-Jun Mao,E-mail:maozhujun0107@163.com;Si-Wei Wang,E-mail:358031289@qq.com
基金资助:
This work was supported by Zhejiang Provincial Natural Science Foundation of China [LQ22H270014], the Chinese medicine science foundation of Zhejiang Province, China [2020ZA120], Quzhou technology projects, China [2022K53, 2023K113, 2023K120], Science and technology project of Zhejiang Provincial Drug Administration [2021013].

Essential oil extracted from Quzhou Aurantii Fructus prevents acute liver failure through inhibiting lipopolysaccharide-mediated inflammatory response

Tian Lan¹, Wen Wang², De-Lian Huang³, Xi-Xi Zeng¹, Xiao-Xiao Wang⁴, Jian Wang⁴, Yu-Hua Tong^1,5, Zhu-Jun Mao^5,6, Si-Wei Wang^1,5

1. The Joint Innovation Center for Health and Medicine, Quzhou People's Hospital, The Quzhou Affiliated Hospital of Wenzhou Medical University, No. 100 Minjiang Road, Quzhou, 324000, China;
2. Preventive Treatment Center, Zhejiang Chinese Medical University Affiliated Four-provinces Marginal Hospital of Traditional Chinese Medicine, Quzhou Hospital of Traditional Chinese Medicine, Quzhou, 324000, China;
3. School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou, 310053, China;
4. Department of Drug Analysis Center, Quzhou Institute for Food and Drug Control, Quzhou, 324000, China;
5. Department of Ophthalmology, Quzhou People's Hospital, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou, 324000, China;
6. College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, No. 548 Binwen Road, Hangzhou, 310053, China

Received:2023-07-08 Online:2023-10-24 Published:2023-11-03
Contact: Yu-Hua Tong,E-mail:yuhuatong@126.com;Zhu-Jun Mao,E-mail:maozhujun0107@163.com;Si-Wei Wang,E-mail:358031289@qq.com
Supported by:
This work was supported by Zhejiang Provincial Natural Science Foundation of China [LQ22H270014], the Chinese medicine science foundation of Zhejiang Province, China [2020ZA120], Quzhou technology projects, China [2022K53, 2023K113, 2023K120], Science and technology project of Zhejiang Provincial Drug Administration [2021013].

摘要/Abstract

摘要： Quzhou Aurantii Fructus (QAF) has a long history as a folk medicine and food for the treatment of liver diseases. While our earlier study provided evidence of hepatoprotective properties contained within the flavonoids and limonins constituents in QAF, the potential preventative effects afforded by essential oil components present within QAF remains enigmatic. In this study, we prepared Quzhou Aurantii Fructus essential oil (QAFEO) and confirmed its anti-inflammatory effects on liver inflammation through experimentation on lipopolysaccharide and D-galactosamine (LPS/D-GalN) induced acute liver failure (ALF) mouse models. Using RNA-sequence (RNA-seq) analysis, we found that QAFEO prevented ALF by systematically blunting the pathways involved in response to LPS and toll-like receptor signaling pathways. QAFEO effectively suppressed the phosphorylation of tank-binding kinase 1 (TBK1), TGF-beta activated kinase 1 (TAK1), interferon regulatory factor 3 (IRF3), and the activation of mitogen activated kinase-like protein (MAPK) and nuclear factor-kappa B (NF-κB) pathways in vivo and in vitro. Importantly, QAFEO substantially reduced myeloid differentiation primary response gene 88 (MyD88)- toll-like receptor 4 (TLR4) interaction levels. Moreover, 8 compounds from QAFEO could directly bind to REAL, TAK1, MyD88, TBK1, and IRF3. Taken together, the results of our study support the notion that QAFEO exerts a hepatoprotective effect through inhibiting LPS-mediated inflammatory response.

关键词: Quzhou Aurantii Fructus, Essential oil, Acute liver failure, LPS, Inflammation

Abstract: Quzhou Aurantii Fructus (QAF) has a long history as a folk medicine and food for the treatment of liver diseases. While our earlier study provided evidence of hepatoprotective properties contained within the flavonoids and limonins constituents in QAF, the potential preventative effects afforded by essential oil components present within QAF remains enigmatic. In this study, we prepared Quzhou Aurantii Fructus essential oil (QAFEO) and confirmed its anti-inflammatory effects on liver inflammation through experimentation on lipopolysaccharide and D-galactosamine (LPS/D-GalN) induced acute liver failure (ALF) mouse models. Using RNA-sequence (RNA-seq) analysis, we found that QAFEO prevented ALF by systematically blunting the pathways involved in response to LPS and toll-like receptor signaling pathways. QAFEO effectively suppressed the phosphorylation of tank-binding kinase 1 (TBK1), TGF-beta activated kinase 1 (TAK1), interferon regulatory factor 3 (IRF3), and the activation of mitogen activated kinase-like protein (MAPK) and nuclear factor-kappa B (NF-κB) pathways in vivo and in vitro. Importantly, QAFEO substantially reduced myeloid differentiation primary response gene 88 (MyD88)- toll-like receptor 4 (TLR4) interaction levels. Moreover, 8 compounds from QAFEO could directly bind to REAL, TAK1, MyD88, TBK1, and IRF3. Taken together, the results of our study support the notion that QAFEO exerts a hepatoprotective effect through inhibiting LPS-mediated inflammatory response.

Key words: Quzhou Aurantii Fructus, Essential oil, Acute liver failure, LPS, Inflammation

Tian Lan, Wen Wang, De-Lian Huang, Xi-Xi Zeng, Xiao-Xiao Wang, Jian Wang, Yu-Hua Tong, Zhu-Jun Mao, Si-Wei Wang. Essential oil extracted from Quzhou Aurantii Fructus prevents acute liver failure through inhibiting lipopolysaccharide-mediated inflammatory response[J]. 应用天然产物, 2023, 13(5): 36-36.

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Essential oil extracted from Quzhou Aurantii Fructus prevents acute liver failure through inhibiting lipopolysaccharide-mediated inflammatory response

Essential oil extracted from Quzhou Aurantii Fructus prevents acute liver failure through inhibiting lipopolysaccharide-mediated inflammatory response

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