Puerarin alleviates sleep disorders in aged mice related to repairing intestinal mucosal barrier

doi:10.1007/s13659-023-00390-3

应用天然产物 ›› 2023, Vol. 13 ›› Issue (4): 29-29.DOI: 10.1007/s13659-023-00390-3

• ORIGINAL ARTICLES • 上一篇下一篇

Puerarin alleviates sleep disorders in aged mice related to repairing intestinal mucosal barrier

Qing Tao¹, Jinhua Zhang², Qiao liang¹, Shiyu Song¹, Shuxia Wang², Xiaoming Yao², Qian Gao¹, Lei Wang^1,3

1. Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine, Medical School, Nanjing University, Nanjing, 210093, Jiangsu, China;
2. Department of Clinical Laboratory, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China;
3. Department of Clinical Laboratory, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China

收稿日期:2023-07-10 出版日期:2023-08-24 发布日期:2023-10-08
通讯作者: Qian Gao,E-mail:qian_gao@nju.edu.cn;Lei Wang,E-mail:wlei@nju.edu.cn
基金资助:
We thank all involved for their participation and technical support. We also thank the Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University and the Department of Clinical Laboratory, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese medicine, Jiangsu Province Academy of Traditional Chinese Medicine. The authors wish to express their gratitude for the financial support received from the National Key Research and Development Program of China (No: 2020YFC2005300, No: 2021YFC2009101), Open/Independent Project of the Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, and Jiangsu Province Postgraduate Scientific Research Innovation Project (KYCX22-0183).

Puerarin alleviates sleep disorders in aged mice related to repairing intestinal mucosal barrier

Qing Tao¹, Jinhua Zhang², Qiao liang¹, Shiyu Song¹, Shuxia Wang², Xiaoming Yao², Qian Gao¹, Lei Wang^1,3

1. Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine, Medical School, Nanjing University, Nanjing, 210093, Jiangsu, China;
2. Department of Clinical Laboratory, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China;
3. Department of Clinical Laboratory, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China

Received:2023-07-10 Online:2023-08-24 Published:2023-10-08
Contact: Qian Gao,E-mail:qian_gao@nju.edu.cn;Lei Wang,E-mail:wlei@nju.edu.cn
Supported by:
We thank all involved for their participation and technical support. We also thank the Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University and the Department of Clinical Laboratory, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese medicine, Jiangsu Province Academy of Traditional Chinese Medicine. The authors wish to express their gratitude for the financial support received from the National Key Research and Development Program of China (No: 2020YFC2005300, No: 2021YFC2009101), Open/Independent Project of the Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, and Jiangsu Province Postgraduate Scientific Research Innovation Project (KYCX22-0183).

摘要/Abstract

摘要： More and more evidence suggests that puerarin, a potential remedy for gut inflammation, may have an ameliorative effect on sleep disturbances. However, the relationship between puerarin and sleep disruption has not been extensively researched. This study aims to explore the role and mechanisms of puerarin in improving sleep disorders. We established a light-induced sleep disorder model in mice and assessed the effects of puerarin on cognitive behavior using open field and water maze tests. Pathological detection demonstrated that sleep disturbances resulted in observable damage to the liver, lung, and kidney. Puerarin reversed multi-organ damage and inflammation. Further, puerarin activated paneth cells, resulting in increased lysozyme and TGF-β production, and stimulating intestinal stem cell proliferation. Puerarin also effectively inhibited the expression of F4/80, iNOS, TNF-α, and IL-1β in the small intestine, while it increased Chil3, CD206, and Arg-1 levels. Moreover, puerarin treatment significantly decreased P-P65, TLR4, Bcl-xl, and cleaved caspase-3 protein levels while increasing barrier protein levels, including ZO-1, Occludin, Claudin 1 and E-cadherin suggesting a reduction in inflammation and apoptosis in the gut. Overall, puerarin diminished systemic inflammation, particularly intestinal inflammation, and enhanced intestinal barrier integrity in mice with sleep disorders. Our findings suggest a potential new therapeutic pathway for sleep disorders.

关键词: Intestinal inflammation, Intestinal mucosal barrier, Light-induced sleep disorder, Puerarin

Abstract: More and more evidence suggests that puerarin, a potential remedy for gut inflammation, may have an ameliorative effect on sleep disturbances. However, the relationship between puerarin and sleep disruption has not been extensively researched. This study aims to explore the role and mechanisms of puerarin in improving sleep disorders. We established a light-induced sleep disorder model in mice and assessed the effects of puerarin on cognitive behavior using open field and water maze tests. Pathological detection demonstrated that sleep disturbances resulted in observable damage to the liver, lung, and kidney. Puerarin reversed multi-organ damage and inflammation. Further, puerarin activated paneth cells, resulting in increased lysozyme and TGF-β production, and stimulating intestinal stem cell proliferation. Puerarin also effectively inhibited the expression of F4/80, iNOS, TNF-α, and IL-1β in the small intestine, while it increased Chil3, CD206, and Arg-1 levels. Moreover, puerarin treatment significantly decreased P-P65, TLR4, Bcl-xl, and cleaved caspase-3 protein levels while increasing barrier protein levels, including ZO-1, Occludin, Claudin 1 and E-cadherin suggesting a reduction in inflammation and apoptosis in the gut. Overall, puerarin diminished systemic inflammation, particularly intestinal inflammation, and enhanced intestinal barrier integrity in mice with sleep disorders. Our findings suggest a potential new therapeutic pathway for sleep disorders.

Key words: Intestinal inflammation, Intestinal mucosal barrier, Light-induced sleep disorder, Puerarin

Qing Tao, Jinhua Zhang, Qiao liang, Shiyu Song, Shuxia Wang, Xiaoming Yao, Qian Gao, Lei Wang. Puerarin alleviates sleep disorders in aged mice related to repairing intestinal mucosal barrier[J]. 应用天然产物, 2023, 13(4): 29-29.

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Puerarin alleviates sleep disorders in aged mice related to repairing intestinal mucosal barrier

Puerarin alleviates sleep disorders in aged mice related to repairing intestinal mucosal barrier

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