关键词: 毛草龙, 壮药, 丁香蓼属, α-托可醌, 细胞毒活性

Abstract: To study the chemical constituents of Ludwigia octovalvis, the 80% ethanol extract from L.octovalvis was isolated by silica gel, Sephadex LH-20, Flash C₁₈ and semi-preparative liquid chromatography, and the structures of obtained compounds were identified by physicochemical properties and spectral data. The cytotoxic activity of the isolates were evaluated by MTS method. The results were as follows:(1)Twenty compounds were isolated from L. octovalvis, and their structures were identified as(-)-lyoniresinol(1), 8,8'-bisdihydrosiringenin(2), 5-methoxy-(-)-isolariciresinol(3),(-)-isolariciresinol(4), 3,4'-di-O-methylellagic acid(5), 3,3',4'-tri-O-methylellagic acid(6), 1,3,6-tri-O-galloyl-β-glucospyranose(7), corilagin(8), methyl gallate(9), ethyl gallate(10), terminaliate A(11), syringic acid(12), 3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone(13), luteolin(14), kaempferol(15), 5,8-dihydroxy-7-methoxyflavone(16), nobiletin(17), tangeretin(18), α-tocopherolquinone(19), 5-O-(E)-p-coumaroyl quinic acid ethyl ester(20). Compounds 1-5, 7, 8, 11, 13, 16-20 were isolated from Ludwigia for the first time. Compounds 6, 9, 10, 12, 15 were isolated from this plant for the first time.(2)Compound 19 showed inhibitory activity against the leukemia HL-60 with the IC₅₀ value of 10.31 μmol·L^-1. Compounds 6-8, 19 showed inhibitory activity against the lung cancer cells A549 with the IC₅₀ values of 25.82, 42.05, 36.94, 17.54 μmol·L^-1, respectively. Compounds 6, 7, 11, 14, 19 showed inhibitory activity against the liver cancer SMMC-7721 with the IC₅₀ values of 24.24, 26.35, 26.51, 33.34, 20.44 μmol·L^-1, respectively. Compounds 6, 19 showed inhibitory activity against the breast cancer MDA-MB-231 with the IC₅₀ values of 34.91, 21.13 μmol·L^-1, respectively. Compounds 6, 7, 19 showed inhibitory activity against the colon cancer SW480 with the IC₅₀ values of 36.03, 39.97, 5.52 μmol·L^-1, respectively. The results of this study enrich the chemical constituents of L. octovalvis and provide a basis for the development of anti-tumor activity.

Key words: Ludwigia octovalvis, Zhuang Medicine, Ludwigia, α-tocopherolquinone, cytotoxic activity