关键词: 宽叶金粟兰, 化学成分, 分离纯化, 结构鉴定, 细胞毒活性, 抑作用

Abstract: In order to study the chemical constituents from Chloranthus henryi and their antitumor activities in vitro. The chemical constituents of ethyl acetate fraction, extracted with 95% ethanol from C. henryi were isolated and purified by silica gel column chromatography, reversed phase column chromatography, Sephadex LH-20 column chromatography and preparation liquid chromatography, and their structures were identified by the physicochemical properties, spectral data combined with relevant literatures. The cytotoxic activities of these compounds were evaluated by MTT method. The results were as follows:(1)Twelve compounds were isolated and identified as pipercyclobutanamide C(1), chololactone A(2), sarcanolide B(3), oxacol A(4), chloramultiol D(5), chlorasessilifol B(6), chlorajaponol(7), tianmushanol(8), spicachlorantins B(9), spicachlorantins A(10), serrachlorin A(11), chloramultiols A(12). Among them, Compound 1 was identified as a new compound, and except for Compound 8, all the other compounds were isolated from the C. henryi for the first time.(2)The cytotoxic activity test results showed that only compounds 2 and 7 had good inhibitory effects on HeLa cells in vitro, while the other compounds showed no inhibition effects. The IC₅₀ of the two compounds were(4.50±0.27)μmol·L^-1 and(4.25±0.08)μmol·L^-1 respectively. In conclusion, the study enriches the chemical constituents of C. henryi, and provides a reference for the further exploration and utilization of this Chloranthus herb plants.

Key words: Chloranthus henryi, chemical constituents, isolation and purification, structural identification, cytotoxic activity, inhibitory effects