Effects of Drought Stress on Leaf Structure of Eucommia ulmoides

doi:10.7525/j.issn.1673-5102.2021.06.013

Abstract

Abstract:

To explore the effects of drought stress on leaf structure of Eucommia ulmoides and understand the characteristics of drought adaptation， 2-year-old cutting seedlings of ‘Huazhong No.6’ were used as experimental materials. The results showed the leaf relative water content， sponge tissue thickness and lower epidermis thickness were significantly lower on the 16th day of drought stress compared with the control group respectively. During the drought stress， the stomatal density of leaves increased at first and then decreased， while the stomatal size expressed by stomatal area， stomatal length and stomatal width pointed a trend of “increase-decrease-increase” and there was a significant negative linear correlation among stomatal length and width and stomatal density. The main reason of the cutting seedling thickness thinning of Eucommia ulmoides was that the sponge tissue thickness became smaller. Under drought stress for 11 days， the stomata of leaves were small and dense， the stomata closure increased， while the stomata showed larger and sparser adaptation characteristics for 16 days under drought stress.

Key words: Eucommia ulmoides, drought stress, leaf structure, stomata

CLC Number:

S723.31

Feng HE, Hong-Yan DU, Pan-Feng LIU, Lu WANG, Jun QING, Lan-Ying DU. Effects of Drought Stress on Leaf Structure of Eucommia ulmoides[J]. Bulletin of Botanical Research, 2021, 41(6): 947-956.

References

1	Chen Q，Tao S Y，Bi X H，et al.Research progress in physiological and molecular biology mechanism of drought resistance in rice［J］.American Journal of Molecular Biology，2013，3（2）：102-107.
2	曹林青，钟秋平，罗帅，等.干旱胁迫下油茶叶片结构特征的变化［J］.林业科学研究，2018，31（3）：136-143.
2	Cao L Q，Zhong Q P，Luo S，et al.Variation in leaf structure of Camellia oleifera under drought stress［J］.Forest Research，2018，31（3）：136-143.
3	李中华，刘进平，谷海磊，等.干旱胁迫对植物气孔特性影响研究进展［J］.亚热带植物科学，2016，45（2）：195-200.
3	Li Z H，Liu J P，Gu H L，et al.Review on the effects of drought stress on plant stomatal characteristics［J］.Subtropical Plant Science，2016，45（2）：195-200.
4	赵雅丽，熊浩，毕玉芬，等.干热胁迫对紫花苜蓿叶片气孔大小的影响［J］.云南农业大学学报，2013，28（3）：336-339.
4	Zhao Y L，Xiong H，Bi Y F，et al.Effect of drought and heat stress on leaf stomata size in alfalfa［J］.Journal of Yunnan Agricultural University，2013，28（3）：336-339.
5	黄绢，陈存，张伟溪，等.干旱胁迫对转JERF36银中杨苗木叶片解剖结构及光合特性的影响［J］.林业科学，2017，53（5）：8-15.
5	Huang J，Chen C，Zhang W X，et al.Effects of drought stress on anatomical structure and photosynthetic characteristics of transgenic JERF36 Populus alba×P.berolinensis seedling leaves［J］.Scientia Silvae Sinicae，2017，53（5）：8-15.
6	董晓民，刘伟，李桂祥，等.干旱胁迫下两个扁桃品种的叶片解剖结构分析［J］.黑龙江农业科学，2018（12）：54-57.
6	Dong X M，Liu W，Li G X，et al.Drought resistance analysis of two almond spieces［J］.Heilongjiang Agricultural Sciences，2018（12）：54-57.
7	庞杰，张凤兰，郝丽珍，等.沙芥幼苗叶片解剖结构和光合作用对干旱胁迫的响应［J］.生态环境学报，2013，22（4）：575-581.
7	Pang J，Zhang F L，Hao L Z，et al.Effect of drought stress on anatomical structure and photosynthesis of Pugionium cornutum（L.） Gaertn.leaves in seedling［J］.Ecology and Environment Sciences，2013，22（4）：575-581.
8	高彦萍，冯莹，马志军，等.水分胁迫下不同抗旱类型大豆叶片气孔特性变化研究［J］.干旱地区农业研究，2007，25（2）：77-79.
8	Gao Y P，Feng Y，Ma Z J，et al.Stomatal character changes of soybean leaves under water stress［J］.Agricultural Research in the Arid Areas，2007，25（2）：77-79.
9	刘世鹏，刘济明，曹娟云，等.干旱胁迫下枣树叶片表皮气孔分布及特征分析［J］.安徽农业科学，2006，34（7）：1315-1318.
9	Liu S P，Liu J M，Cao J Y，et al.Stomatal distribution and character analysis of leaf epidermis of jujube under drought stress［J］.Journal of Anhui Agricultural Sciences，2006，34（7）：1315-1318.
10	Chartzoulakis K，Bosabalidis A，Patakas A，et al.Effects of water stress on water relations，gas exchange and leaf structure of olive tree［J］.Acta Horticulturae，2000，537（25）：241-247.
11	刘球，吴际友，李志辉.干旱胁迫对植物叶片解剖结构影响研究进展［J］.湖南林业科技，2015，42（3）：101-104.
11	Liu Q，Wu J Y，Li Z H.Research progress of leaf anatomical structure of plants under drought stress［J］.Hunan Forestry Science & Technology，2015，42（3）：101-104.
12	刘红云，梁宗锁，刘淑明，等.持续干旱及复水对杜仲幼苗保护酶活性和渗透调节物质的影响［J］.西北林学院学报，2007，22（3）：55-59.
12	Liu H Y，Liang Z S，Liu S M，et al.Effect of progressive drying and rewatering on protective enzyme activities and osmoregulatory molecules in leaves of Eucommia ulmoides seeding［J］.Journal of Northwest Forestry University，2007，22（3）：55-59.
13	伍江波，金晓玲，汪晓丽，等.盐胁迫对杜仲幼苗生理特性的影响［J］.广东农业科学，2015，42（2）：17-21.
13	Wu J B，Jin X L，Wang X L，et al.Effects of salt stress on physiological characteristics of Eucommia ulmoides seedlings［J］.Guangdong Agricultural Sciences，2015，42（2）：17-21.
14	杨雨华，宗建伟，王键胜，等.高温胁迫对杜仲幼苗部分生理指标的影响［J］.东北林业大学学报，2015，43（3）：14-17.
14	Yang Y H，Zong J W，Wang J S，et al.Effects of high temperature stress on physiological indexes of Eucommia ulmoides seedlings［J］.Journal of Northeast Forestry University，2015，43（3）：14-17.
15	张明艳.杜仲对盐胁迫反应的研究［D］.兰州：甘肃农业大学，2000.
15	Zhang M Y.Studies on effects of Eucommia ulmoides Oliver under salt stress［D］.Lanzhou：Gansu Agricultural University，2000.
16	高翱.杜仲和三个耐盐树种对盐碱混合胁迫的响应与耐盐碱性研究［D］.晋中：山西农业大学，2017.
16	Gao A.Research on the response of Eucommia ulmoides and three salt-tolerant trees under saline-alkali mix stress and their saline-alkali tolerance［D］.Jinzhong：Shanxi Agricultural University，2017.
17	张荣荣.土壤干旱下杜仲截干苗生长和光合生理响应及引种栽培研究［D］.晋中：山西农业大学，2016.
17	Zhang R R.Eucommia ulmoides stem cutting seedling growth and photosynthetic physiological response under soil drought and study on introduction and cultivation［D］.Jinzhong：Shanxi Agricultural University，2016.
18	杨全，孟平，李俊清，等.土壤水分胁迫对杜仲叶片光合及水分利用特征的影响［J］.中国农业气象，2010，31（1）：48-52.
18	Yang Q，Meng P，Li J C，et al.Effect of soil water stress on photosynthetic and transpiration characters of Eucommia ulmoides［J］.Chinese Journal of Agrometeorology，2010，31（1）：48-52.
19	何斐，哀建国，朱向涛.水杨酸、三唑酮对水淹胁迫下杜仲光合特性的影响［J］.浙江林业科技，2016，36（1）：14-18.
19	He F，Ai J G，Zhu X T.Effect of salicylic acid and triazolone on photosynthetic properties of Eucommia ulmoides under water logging stress［J］.Journal of Zhejiang Forestry Science and Technology，2016，36（1）：14-18.
20	谈锋，周彦兵，梁莉.杜仲叶片对土壤水分胁迫的适应性研究［J］.西南师范大学学报：自然科学版，1996，21（3）：266-270.
20	Tan F，Zhou Y B，Liang L.A study on the adaptability of Eucommia ulmoides leaves to soil water stress［J］.Journal of Southwest China Normal University：Natural Science，1996，21（3）：266-270.
21	曾惠敏，赵冰.28个八仙花品种叶片解剖结构与植株耐旱性的关系［J］.东北林业大学学报，2019，47（1）：10-19.
21	Zeng H M，Zhao B.Relationship between leaf anatomical structure and drought tolerance of 28 Hydrangea Cultivars［J］.Journal of Northeast Forestry University，2019，47（1）：10-19.
22	郭文文，卓么草，周尧治.西藏高原硬叶柳叶片结构对寒旱环境的适应机制［J］.西北植物学报，2019，39（5）：784-790.
22	Guo W W，Zhuo M C，Zhao Y Z.The Salix sclerophylla leaves to adapt to the cold and drought environment on the Tibetan plateau［J］.Acta Botanica Boreali-Occidentalia Sinica，2019，39（5）：784-790.
23	叶昌辉，陈丹，郑晓婷，等.桃金娘叶片的解剖结构及其生态适应性研究［J］.安徽农业科学，2019，47（2）：1-3.
23	Ye C H，Chen D，Zheng X T，et al.Study on the anatomical structure of Rhodomyrtus tomentosa leaf blade and its ecological adaptability［J］.Journal of Anhui Agricultural Sciences，2019，47（2）：1-3.
24	可静，李进，吕海英，等.不同条件下黑果枸杞叶片气孔开度和超微结构的变化［J］.干旱区研究，2017，34（6）：1362-1370.
24	Ke J，Li J，Lv H Y，et al.Change of stomatal aperture and ultrastructure on Lycium ruthenicum Murr.leaves under different conditions［J］.Arid Zone Research，2017，34（6）：1362-1370.
25	Bacelar E A，Correia C M，Moutinho-Pereira J M，et al.Sclerophylly and leaf anatomical traits of five field-grown olive cultivars growing under drought conditions［J］.Tree Physiology，2004，24（2）：233-239.
26	Chartzoulakis K，Patakas A，Kofidis G，et al.Water stress affects leaf anatomy，gas exchange，water relations and growth of two avocado cultivars［J］.Scientia Horticulturae，2002，95（1-2）：39-50.
27	常英俏，徐文远，穆立蔷，等.干旱胁迫对3种观赏灌木叶片解剖结构的影响及抗旱性分析［J］.东北林业大学学报，2012，40（3）：36-40.
27	Chang Y Q，Xu W Y，Mu L Q，et al.Effects of drought stress on anatomical structure of leaves of three species of shrubs and their drought resistances［J］.Journal of Northeast Forestry University，2012，40（3）：36-40.
28	杨秀平，周玮，李煜，等.杜仲无性系叶片解剖结构及抗旱性评价［J］.西北林学院学报，2016，31（1）：119-125.
28	Yang X P，Zhou W，Li Y，et al.Drought resistance and leaf anatomical structure of Eucommia ulmoides clones［J］.Journal of Northwest Forestry University，2016，31（1）：119-125.
29	王光耀，刘俊梅，张仪，等.菜豆4个不同抗热性品种的气孔特性［J］.农业生物技术学报，1999，7（3）：267-270.
29	Wang G Y，Liu J M，Zhang Y，et al.Studies on stomatal properties of four Common Bean cultivars with known different thermoresistance［J］.Journal of Agricultural Biotechnology，1999，7（3）：267-270.
30	陈安和.高温影响水稻叶片气孔开闭的扫描电镜观察［J］.渝州大学学报：自然科学版，1989（3）：45-49.
30	Chen A H.Observation of the states showing opening and closing stomata of the hybrid rice cultivar under the different temperatures with scanning electron microscope［J］.Journal of Yuzhou University，1989（3）：45-49.
31	张青，彭祚登.7叶树幼苗对水分胁迫和复水的生理响应［J］.中南林业科技大学学报，2018，38（4）：46-53.
31	Zhang Q，Peng Z D.Physiological response to water stress and rewatering of Aesculus chinensis seedlings［J］.Journal of Central South University of Forestry & Technology，2018，38（4）：46-53.

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