Effects of Drought Stress on Photosynthetic and Physiological Characteristics of Juglans mandshurica Seedlings in Different Soil Substrates

doi:10.7525/j.issn.1673-5102.2019.05.011

Abstract

Abstract: To investigate the response of photosynthetic physiological characteristics in seedlings of Juglans mandshurica under water stress in different soil substrates, In this study, two-year seedlings of J.mandshurica were used as research objects, and an experiment was conducted to set four water gradients(normal water supply CK, mild stress T1, moderate stress T2 and severe stress T3, and 80%, 60%, 40%, 20% field water capacity, respectively) in three soil substrates(humus, loam, sandy-loam) by pot controlled water method respectively. The chlorophyll, malondialdehyde(MDA), proline(Pro), antioxidant enzymes(POD, SOD, CAT)and photosynthesis indicators were determined in all treatments. The results showed that the chlorophyll content and Chl a/b were significantly decreased with increase of stress intensity(P<0.05). After 60 d of persistent water stress, the Chl a and Chl b content were 65.41% and 51.57% lower than those of CK, respectively. The order of chlorophyll content in the three matrices was:humus>loam>sandy-loam. With the increase of stress intensity, the proline(Pro) and malondialdehyde(MDA) content of seedlings were significantly increased, and Pro content in T3 in sandy-loam soil was 83.37 mg·g^-1, which was highest among all treatment, and was 86.32% higher than CK. The activities of superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) of seedlings significantly increased with increase of stress intensity in humus soil. However, the SOD activity in loam soil increased first and then decreased. In general, the net photosynthetic rate(P_n), transpiration rate(T_r) and stomatal conductance(G_s) of seedlings decreased with increase of the stress intensity in three soil substrates. However, the stomatal limitation and non-stomatal limitation of seedling leaves in different soil substrates were different. The intercellular CO₂ concentration(C_i) of T3 treated leaves was lowest in humus at 12:00, while in loam and sandy loam were 10:00. There was a significant positive correlation between soil total nitrogen, total phosphorus, permeation rate and transpiration rate and chlorophyll content(P<0.05), and reach a significant negative correlation between soil bulk density and chlorophyll content(P<0.05). Soil substrate and water stress had significant effects on the physiological characteristics and photosynthesis of J.mandshurica seedlings. Severe stress caused serious damage to the chlorophyll, osmotic substances and enzyme system of seedlings, and decreased the photosynthetic productivity of J.mandshurica seedlings. The seedlings in humus and loam have better drought resistance.

Key words: soil substrate, drought stress, photosynthesis, physiological characteristics, Juglans mandshurica

CLC Number:

S723.1

JI Li, HAN Jiao, WANG Fang, WANG Jun, SONG Di, ZHANG Li-Jie, QI Yong-Hui, YANG Yu-Chun. Effects of Drought Stress on Photosynthetic and Physiological Characteristics of Juglans mandshurica Seedlings in Different Soil Substrates[J]. Bulletin of Botanical Research, 2019, 39(5): 722-732.

References

1. Vitousek P M,Aber J D,Howarth R W,et al. Technical report:human alteration of the global nitrogen cycle:sources and consequences[J]. Ecological Applications,1997,7(3):737-750.
2. 高悦,朱永铸,杨志民,等. 干旱胁迫和复水对冰草相关抗性生理指标的影响[J]. 草地学报,2012,20(2):336-341. Gao Y,Zhu Y Z,Yang Z M,et al. Effects of drought stress and recovery on antioxidant enzyme activities of Agropyron cristatum[J]. Acta Agrestia Sinica,2012,20(2):336-341.
3. Chaves M M,Oliveira M M. Mechanisms underlying plant resilience to water deficits:prospects for water-saving agriculture[J]. Journal of Experimental Botany,2004,55(407):2365-2384.
4. Bréda N,Huc R,Granier A,et al. Temperate forest trees and stands under severe drought:a review of ecophysiological responses,adaptation processes and long-term consequences[J]. Annals of Forest Science,2006,63(6):625-644.
5. 李力,刘玉民,王敏,等. 3种北美红枫对持续高温干旱胁迫的生理响应机制[J]. 生态学报,2014,34(22):6471-6480. Li L,Liu Y M,Wang M,et al. Physiological response mechanism of three kinds of Acer rubrum L. under continuous high temperature and drought stress[J]. Acta Ecologica Sinica,2014,34(22):6471-6480.
6. 王玉魁,阎艳霞,余新晓,等. 干旱胁迫下灰白滨藜保护酶活性及抗旱生理特性研究[J]. 干旱区资源与环境,2010,24(10):122-126. Wang Y K,Yan Y X,Yu X X,et al. Activity of protective enzymes of Atriplex canescens and its drought resistance physiological characters under drought stress[J]. Journal of Arid Land Resourcesand Environment,2010,24(10):122-126.
7. 邹春静,徐文铎,靳牡丹,等. 干旱胁迫对沙地云杉生态型保护酶活性的影响[J]. 干旱区研究,2007,24(6):810-814. Zou C J,Xu W D,Jin M D,et al. Study on the effectof drought stress on the activity of protective enzyme of three ecotypes of Picea mongolica[J]. Arid Zone Research,2007,24(6):810-814.
8. 王曦,胡红玲,胡庭兴,等. 干旱胁迫对桢楠幼树渗透调节与活性氧代谢的影响及施氮的缓解效应[J]. 植物生态学报,2018,42(2):240-251. Wang X,Hu H L,Hu T X,et al. Effects of drought stress on the osmotic adjustment and active oxygen metabolism of Phoebe zhennan seedlings and its alleviation by nitrogen application[J]. Chinese Journal of Plant Ecology,2018,42(2):240-251.
9. 李瑞姣,陈献志,岳春雷,等. 干旱胁迫对日本荚蒾幼苗光合生理特性的影响[J]. 生态学报,2018,38(6):2041-2047. Li R J,Chen X Z,Yue C L,et al. Effects of drought stress on the photosynthetic characteristics of Viburnum japonicum seedlings[J]. Acta Ecologica Sinica,2018,38(6):2041-2047.
10. Bloemen J,Vergeynst L L,Overlaet-Michiels L,et al. How important is woody tissue photosynthesis in poplar during drought stress?[J]. Trees,2016,30(1):63-72.
11. Chaves M M,Flexas J,Pinheiro C. Photosynthesis under drought and salt stress:regulation mechanisms from whole plant to cell[J]. Annals of Botany,2009,103(4):551-560.
12. Ge Y,Lu Y J,Liao J X,et al. Photosynthetic parameters of Mosla hangchowensis and M. dianthera as affected by soil moisture[J]. Photosynthetica,2004,42(3):387-391.
13. 张刚,魏典典,邬佳宝,等. 干旱胁迫下不同种源文冠果幼苗的生理反应及其抗旱性分析[J]. 西北林学院学报,2014,29(1):1-7,50. Zhang G,Wei D D,Wu J B,et al. Effects of drought stress on physiological characteristics of Xanthoceras sorbifolia from different provenances and analysis on drought-resistance in seedling stage[J]. Journal of Northwest Forestry University,2014,29(1):1-7,50.
14. 曹弈磷,曾春菡,刘小波,等. PEG模拟干旱胁迫下4种苔藓植物的生理指标变化及其耐旱性评价[J]. 西北林学院学报,2014,29(4):33-39. Cao Y L,Zeng C H,Liu X B,et al. Physiological and biochemical response and drought resistance evaluation of four wild bryophytes[J]. Journal of Northwest Forestry University,2014,29(4):33-39.
15. 姜瑞芳,刘艳红. 土壤基质和水分对珙桐幼苗生长的影响[J]. 西北林学院学报,2016,31(4):134-139,164. Jiang R F,Liu Y H. Effects of soil matrix and moisture on the growth of Davidia involucrata seedlings[J]. Journal of Northwest Forestry University,2016,31(4):134-139,164.
16. 刘晚苟. 不同土壤水分条件下容重对玉米生长和水分利用的调控[D]. 杨凌:西北农林科技大学,2001. Liu W G. Regulation of maize plant growth and water use by varying soil bulk density under different soil water conditions[D]. Yangling:Northwest A&F University,2001.
17. 姚小兰,周琳,冯茂松,等. 干旱胁迫对不同基质网袋桢楠幼苗生长及生物量的影响[J]. 植物研究,2018,38(1):81-90. Yao X L,Zhou L,Feng M S,et al. Effects of drought stress on the growth and biomass of Phoebe zhennan seedling in different substrates net container[J]. Bulletin of Botanical Research,2018,38(1):81-90.
18. 陈思羽,杨辉,韩姣,等. 长白山区核桃楸结实性状种源变异分析[J]. 北京林业大学学报,2015,37(12):32-40. Chen S Y,Yang H,Han J,et al. Provenance variation of seed traits of Juglans mandshurica in Changbai mountains,northeastern China[J]. Journal of Beijing Forestry University,2015,37(12):32-40.
19. 赵珊珊,张大伟,许延国,等. 核桃楸不同种源苗期生长的研究[J]. 吉林林业科技,2016,45(1):4-9. Zhao S S,Zhang D W,Xu Y G,et al. The research of different provenance seedling growth of Juglans mandshurica[J]. Journal of Jilin Forestry Science and Technology,2016,45(1):4-9.
20. 林士杰,张大伟,杨辉,等. 长白山区核桃楸芽接技术研究[J]. 吉林林业科技,2016,45(1):13-15,27. Lin S J,Zhang D W,Yang H,et al. The budding technology research of Juglans mandshurica in Changbai Mountain[J]. Journal of Jilin Forestry Science and Technology,2016,45(1):13-15,27.
21. 孙一荣,朱教君,于立忠,等. 不同光强下核桃楸、水曲柳和黄菠萝的光合生理特征[J]. 林业科学,2009,45(9):29-35. Sun Y R,Zhu J J,Yu L Z,et al. Photosynthetic characteristics of Juglans mandshurica,Fraxinus mandshurica and Phellodendron amurense under different light regimes[J]. Scientia Silvae Sinicae,2009,45(9):29-35.
22. Yang N,Ji L,Salahuddin,et al. The influence of tree species on soil properties and microbial communities following afforestation of abandoned land in northeast China[J]. European Journal of Soil Biology,2018,85:73-78.
23. 陈立新. 土壤实验实习教程[M]. 哈尔滨:东北林业大学出版社,2005. Chen L X. Soil experiment practice tutorial[M]. Harbin:Northeast Forestry University Press,2005.
24. 卫星,李贵雨,吕琳. 农林废弃物育苗基质的保水保肥效应[J]. 林业科学,2015,51(12):26-34. Wei X,Li G Y,Lü L. Water and nutrient preservation of agri-forest residues used as nursery matrix[J]. Scientia Silvae Sinicae,2015,51(12):26-34.
25. 李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社,2000. Li H S. Principles and techniques of plant physiological biochemical experiment[M]. Beijing:Higher Education Press,2000.
26. 毛海颖. 栓皮栎需水规律及灌溉制度的研究[D]. 北京:北京林业大学,2010. Mao H Y. Studies on water utiliation and irrigations of Quercus variabilis seedlings[D]. Beijing:Beijing Forestry University,2010.
27. 桑子阳,马履一,陈发菊. 干旱胁迫对红花玉兰幼苗生长和生理特性的影响[J]. 西北植物学报,2011,31(1):109-115. Sang Z Y,Ma L Y,Chen F J. Growth and physiological characteristics of Magnolia wufengensis seedlings under drought stress[J]. Acta Botanica Boreali-Occidentalia Sinica,2011,31(1):109-115.
28. 杨建伟,赵丹,孙桂芳,等. 干旱胁迫对小紫珠光合生理的影响[J]. 西北植物学报,2018,38(4):733-740. Yang J W,Zhao D,Sun G F,et al. Photosynthetic characteristics of Callicarpa dichotoma underdrought stress[J]. Acta Botanica Boreali-Occidentalia Sinica,2018,38(4):733-740.
29. 李芳兰,包维楷,吴宁. 白刺花幼苗对不同强度干旱胁迫的形态与生理响应[J]. 生态学报,2009,29(10):5406-5416. Li F L,Bao W K,Wu N. Morphological and physiological responses of current Sophora davidii seedlings to drought stress[J]. Acta Ecologica Sinica,2009,29(10):5406-5416.
30. 李阳,齐曼·尤努斯,祝燕. 水分胁迫对大果沙枣光合特性及生物量分配的影响[J]. 西北植物学报,2006,26(12):2493-2499. Li Y,Qiman Y N S,Zhu Y. Effects of water stress on photosynthetic characteristics and biomass partition of Elaeagnus moorcroftii[J]. Acta Botanica Boreali-Occidentalia Sinica,2006,26(12):2493-2499.
31. 李清芳,马成仓,尚启亮. 干旱胁迫下硅对玉米光合作用和保护酶的影响[J]. 应用生态学报,2007,18(3):531-536. Li Q F,Ma C C,Shang Q L. Effects of silicon on photosynthesis and antioxidative enzymes of maize under drought stress[J]. Chinese Journal of Applied Ecology,2007,18(3):531-536.
32. 安玉艳,梁宗锁,郝文芳. 杠柳幼苗对不同强度干旱胁迫的生长与生理响应[J]. 生态学报,2011,31(3):716-725. An Y Y,Liang Z S,Hao W F. Growth and physiological responses of the Periploca sepium Bunge seedlings to drought stress[J]. Acta Ecologica Sinica,2011,31(3):716-725.
33. 贾瑞丰,徐大平,杨曾奖,等. 干旱胁迫对降香黄檀幼苗光合生理特性的影响[J]. 西北植物学报,2013,33(6):1197-1202. Jia R F,Xu D P,Yang Z J,et al. Photosynthesis physiological characteristics of Dalbergia odorifera seedlings under drought stress[J]. Acta Botanica Boreali-Occidentalia Sinica,2013,33(6):1197-1202.
34. 王海珍,韩路,徐雅丽,等. 土壤水分梯度对灰胡杨光合作用与抗逆性的影响[J]. 生态学报,2017,37(2):432-442. Wang H Z,Han L,Xu Y L,et al. Effects of soil water gradient on photosynthetic characteristics and stress resistance of Populus pruinosa in the Tarim Basin,China[J]. Acta Ecologica Sinica,2017,37(2):432-442.
35. 史小玲,薛立,任向荣,等. 华南地区4种阔叶幼苗水分胁迫条件下的抗旱性初探[J]. 林业科学研究,2011,24(6):760-767. Shi X L,Xue L,Ren X R,et al. Preliminary study on drought resistance of four broadleaved seedlings under water stress in South China[J]. Forest Research,2011,24(6):760-767.
36. 任磊,赵夏陆,许靖,等. 4种茶菊对干旱胁迫的形态和生理响应[J]. 生态学报,2015,35(15):5131-5139. Ren L,Zhao X L,Xu J,et al. Varied morphological and physiological responses to drought stress among four tea Chrysanthemum cultivars[J]. Acta Ecologica Sinica,2015,35(15):5131-5139.
37. 王红梅,包维楷,李芳兰. 不同干旱胁迫强度下白刺花幼苗叶片的生理生化反应[J]. 应用与环境生物学报,2008,14(6):757-762. Wang H M,Bao W K,Li L F. Physiological and biochemical responses of two-years-old Sophora davidii seedling leaves to different water stresses[J]. Chinese Journal of Applied &Environmental Biology,2008,14(6):757-762.
38. 郭卫华,李波,黄永梅,等. 不同程度的水分胁迫对沙棘幼苗生理生态特征的影响[J]. 植物学报,2003,45(10):1238-1244. Guo W H,Li B,Huang Y M,et al. Effects of different water stresses on eco-physiological characteristics of Hippophae rhamnoides seedlings[J]. Acta Botanica Sinica,2003,45(10):1238-1244.
39. 范苏鲁,苑兆和,冯立娟,等. 水分胁迫下大丽花光合及叶绿素荧光的日变化特性[J]. 西北植物学报,2011,31(6):1223-1228. Fan S L,Yuan Z H,Feng L J,et al. Daily variation characteristics of photosynthesis and chlorophyll fluorescence in dahlia leaves under water stress[J]. Acta Botanica Boreali-Occidentalia Sinica,2011,31(6):1223-1228.

[1]	Jing ZHANG, Weixi ZHANG, Changjun DING, Zhengsai YUAN, Lirui DAI, Xiaohua SU, Yingbai SHEN, Guanzheng QU. Comparative Analysis of Growth, Photosynthetic Physiology and Root Tip Ion Flow Characteristics of Five Poplar Varieties [J]. Bulletin of Botanical Research, 2024, 44(1): 96-106.
[2]	Huiling YAN, Xinxin ZHANG, Xiyang ZHAO, Guanzheng QU, Zhaoning WANG, Rui HAN. Effects of Light Signal on Anthocyanin Biosynthesis and Gene Expression of AmRosea1 Overexpressed 84K Poplar [J]. Bulletin of Botanical Research, 2023, 43(6): 815-825.
[3]	Chaoyang MA, Tianfeng LIU, Xiaoxue LI, Cancan HUO, Lingling FU, Zailiu LI. Interannual Changes in the Effects of Soil Gravel Content on the Growth and Physiology of Handeliodendron bodinieri Seedlings [J]. Bulletin of Botanical Research, 2023, 43(6): 890-899.
[4]	Shuyao ZHUANG, Hengbo XU, Xiaoyu HU, Shang DAI, Yanni ZHANG. Effects of Saline-alkali Stress on Growth and Physiological Characteristics of Color Leaf Heuchera micrantha ‘Silver Fan’ Seedlings [J]. Bulletin of Botanical Research, 2023, 43(4): 520-530.
[5]	Tingting LI, Liu YANG, Xiaoxia LI, Yisong WANG, Xiuwei WANG. Different Nitrogen Forms on the Photosynthetic Characteristics and Growth of Fraxinus mandshurica and Quercus mongolica [J]. Bulletin of Botanical Research, 2023, 43(2): 207-217.
[6]	Mengshuo LI, Yingze LIU, Huan LU, Sheng QIANG. Photosynthetic Capacity Differentiation and Gene Transcription in Different Geographical Populations of Arabidopsis thaliana under Common Garden conditions [J]. Bulletin of Botanical Research, 2023, 43(1): 90-99.
[7]	Zhihui HUANG, Yining ZHANG, Nana LI, Baojiang ZHENG, Yuhong ZHANG. Responses of Supplemental UV-B Radiation to Physiological Properties and Secondary Metabolites of Thlaspi arvense [J]. Bulletin of Botanical Research, 2022, 42(6): 1079-1087.
[8]	Xiwu DU, Jun QIN, Kang YE, Yonghong HU, Yiwei TAO, Yongzheng PENG, Yanxiang SHEN, Yan LIANG, Li ZENG. Effects of Flooding Stress on Photosynthetic Characteristics of Yulania stellata and Its Cultivars [J]. Bulletin of Botanical Research, 2022, 42(3): 483-491.
[9]	Bin WEI, Yi LI, Shiping SU. The Effect of Exogenous Proline on the Stomata of Nitraria tangutorum Leaves under Natural Drought [J]. Bulletin of Botanical Research, 2022, 42(3): 492-501.
[10]	Feng HE, Hong-Yan DU, Pan-Feng LIU, Lu WANG, Jun QING, Lan-Ying DU. Effects of Drought Stress on Leaf Structure of Eucommia ulmoides [J]. Bulletin of Botanical Research, 2021, 41(6): 947-956.
[11]	Shuang-Hui TIAN, He CHENG, Yang ZHANG, Cong LIU, De-An XIA, Zhi-Gang WEI. Genome-wide Identification and Expressional Analysis of Carotenoid Cleavage Dioxygenases(CCD) Gene Family in Populus trichocarpa under Drought and Salt Stress [J]. Bulletin of Botanical Research, 2021, 41(6): 993-1005.
[12]	Dong ZHANG, Yan LIU, Han ZHANG, Zi-Jian ZHANG, Yang WANG, Mei-Cen LIU. Response of Photosynthesis and Leaf Morphological Characteristics to Drought Stress in Glycyrrhiza uralensis [J]. Bulletin of Botanical Research, 2021, 41(3): 449-457.
[13]	Fang WANG, Zhi-Min LU, Jun WANG, Shi-Kai ZHANG, Yu-Xi LI, Shao-Chen LI, Jian-Qiu ZHANG, Yu-Chun YANG. Photosynthetic and Stomatal Characteristics of Pinus koraiensis and P.sibirica under Low Temperature Stress [J]. Bulletin of Botanical Research, 2021, 41(2): 205-212.
[14]	Xiao-Chi YU, Gui-Juan YANG, Ju-Lan DONG, Jun-Hui WANG, Wen-Jun MA, Peng ZHANG. Physiological Responses to Drought Stress of Five Speciesfrom Catalpa Scop [J]. Bulletin of Botanical Research, 2021, 41(1): 44-52.
[15]	Wen-Hai HU, Xiao-Hong YAN, Xiao-Hong LI, Zao-Gui CAO. Effects of 24-Epibrassinolide on the Chlorophyll Fluorescence Transient in Leaves of Pepper under Drought Stress [J]. Bulletin of Botanical Research, 2021, 41(1): 53-59.