Relationship between H2S and ABA Signaling in Arabidopsis thaliana under Drought Stress

doi:10.7525/j.issn.1673-5102.2019.01.013

Abstract

Abstract: With the seedlings of wild type Arabidopsis thaliana(WT), hydrogen sulfide(H₂S) synthase deletion mutant lcd, abscisic acid(ABA) deletion mutant aba1, 0.3 mol·L^-1 mannitol simulated drought stress, we studied the effects of drought stress on ABA content and H₂S content, analyzed their roles and signal relationships in drought tolerance. Drought stress significantly increased the LCD and ABA1 gene relative expressions, contents of H₂S and ABA; Drought stress significantly inhibited the seed germination of lcd and aba1 mutants; Application of NaHS and ABA significantly increased the endogenous H₂S content in WT under drought stress; Application of NaHS could promote the endogenous H₂S production in WT, lcd and aba1, the relative expression of LCD and ABA1 also up-regulated under drought stress. While the application of ABA increased the H₂S content and the LCD, ABA1 relative expressions in WT and aba1 under drought stress, but had no significant effect on the H₂S content and LCD relative expression in lcd. The results showed that the signal molecules H₂S and ABA played a vital role response to the drought stress in Arabidopsis, and H₂S is located at downstream of ABA to participated in the regulation of signaling process.

Key words: Arabidopsis thaliana, drought stress, hydrogen sulfide, abscisic acid

CLC Number:

Q949.748.3

ZHAO Min, WANG Yue-Xuan, XU Yun-Fei, ZHAO Qi-An, LIU Bo, YANG Ning. Relationship between H₂S and ABA Signaling in Arabidopsis thaliana under Drought Stress[J]. Bulletin of Botanical Research, 2019, 39(1): 104-112.

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Relationship between H₂S and ABA Signaling in Arabidopsis thaliana under Drought Stress

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