Detailed Phenotypical Analysis on the Mutant ssr1-2 Encoding a Mitochondrial Protein of Arabidopsis thaliana

doi:10.7525/j.issn.1673-5102.2023.03.012

Abstract

Abstract:

To explore the role of SSR1 gene encoding a functionally unknown mitochondrial protein of Arabidopsis thaliana in plant growth and stress response， ssr1-2 and its suppressor mutants EMS143 and EMS145 were used to track the growth of their roots and aboveground parts， as well as their proline sensitivity and iron homeostasis， and the effects of ssr1-2 short root phenotype on aboveground growth of seedlings were analyzed by micrografting. The results showed that the primary root length of ssr1-2 was shorter and the root architecture was similar to the fibrous root system. The growth of their shoots was also retarded， but appeared considerably later than the short-root phenotype. The results of micrografting revealed that the roots of ssr1-2 could restrict the growth of the wild-type shoots and， and conversely， the shoot of ssr1-2 could also affect the growth of the wild-type roots， but the former had a bigger effect. ssr1-2 showed a hypersensitive phenotype to proline in seed germination， root length and leaf chlorophyll content. In addition， ssr1-2 appeared insensitive to Fe nutrition， that was， the stimulating effect of Fe salt on seedling growth was significantly less than that of wild-type WS， indicating a significant decrease in its ability to utilize Fe. The results suggested that SSR1 might be involved in the regulation of A. thaliana root growth by affecting the utilization of iron nutrient， implying that the impairment of the mitochondrial iron utilization machinery might contribute significantly to the enhanced inhibitory effect of proline on plant growth and development.

Key words: SSR1, proline treatment, iron-sulfur cluster assembly, micrografting, Arabidopsis thaliana

CLC Number:

S335

Yuanyuan CAI, Jibenben XIA, Wenhan YING, Jieyao WANG, Tao XIE, Kongya XING, Xuanjun FENG, Xuejun HUA. Detailed Phenotypical Analysis on the Mutant ssr1-2 Encoding a Mitochondrial Protein of Arabidopsis thaliana[J]. Bulletin of Botanical Research, 2023, 43(3): 421-431.

Figures/Tables 8

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时间 Time /d	相对萌发率 Relative germination rate /%
时间 Time /d	WS	ssr1-2	ssr1-2C	EMS143	EMS145
1	74.80	31.06	79.08	63.11	108.50
2	98.95	96.21	98.91	121.44	100.50
3	99.42	100.52	94.07	99.50	98.89
4	99.22	98.11	89.17	105.07	95.98
5	101.05	98.13	93.24	106.63	98.50
6	101.95	101.01	95.65	111.17	96.48
7	101.23	100.29	95.65	111.17	95.50

生长条件 Growth condition	株系 Line	指标Index
生长条件 Growth condition	株系 Line	主根长 Primary foot length /cm	叶片面积 Leaf area /cm²	叶绿素质量分数 Chlorophyll content /mg·g^-1
MS培养基 MS medium	WS	2.13	0.61	0.72
	ssr1-2	0.37	0.61	0.65
	ssr1-2C	2.16	0.63	0.73
	EMS143	2.01	0.70	0.57
	EMS145	1.98	0.66	0.67
20 mmol·L^-1 Pro的MS培养基 MS medium containg 20 mmol·L^-1 Pro	WS	1.00	0.43	0.27
	ssr1-2	0.16	0.37	0.17
	ssr1-2C	1.04	0.43	0.27
	EMS143	0.44	0.44	0.27
	EMS145	0.42	0.53	0.40

指标 Index	株系 Line	处理条件 Treat condition
指标 Index	株系 Line	处理1 Treat 1	处理2 Treat 2	处理3 Treat 3	处理4 Treat 4	处理5 Treat 5
主根长 Primary foot length /cm	WS	0.61	2.49	2.09	1.90	1.41
	ssr1-2	0.47	0.46	0.46	0.49	0.46
	ssr1-2C	0.63	2.44	1.99	1.95	1.46
	EMS143	0.60	1.90	1.82	1.98	1.68
	EMS145	0.45	1.18	1.25	0.88	0.65
净生长量 Net growth of primary root /cm	WS	0.04	0.03	0.39	0.22	0.35
	ssr1-2	0.06	0.02	0.03	0.01	0.01
	ssr1-2C	0.05	0.03	0.31	0.25	0.29
	EMS143	0.04	0.03	0.26	0.34	0.31
	EMS145	0.03	0.01	0.12	0.16	0.13
侧根长 Lateral root length /cm	WS	0.23	0.45	0.69	0.60	0.41
	ssr1-2	0.29	0.19	0.22	0.25	0.20
	ssr1-2C	0.23	0.45	0.68	0.64	0.42
	EMS143	0.21	0.81	0.80	1.03	0.75
	EMS145	0.20	0.44	0.78	0.81	0.68
侧根密度 Lateral root density/（number·cm^-1）	WS	3.22	1.72	1.91	1.89	2.09
	ssr1-2	4.44	4.67	4.83	4.67	4.10
	ssr1-2C	3.47	1.74	1.89	1.84	2.00
	EMS143	1.71	2.03	3.37	1.64	1.95
	EMS145	2.82	2.19	2.93	3.94	3.23
叶片面积 Leaf area /cm²	WS	0.72	0.73	0.73	0.75	0.75
	ssr1-2	0.67	0.72	0.66	0.63	0.62
	ssr1-2C	0.74	0.76	0.75	0.78	0.77
	EMS143	0.52	0.67	0.67	0.68	0.69
	EMS145	0.52	0.68	0.70	0.67	0.67
叶绿素质量分数 Chlorophyll content /（mg·g^-1）	WS	0.51	1.17	1.12	1.20	1.26
	ssr1-2	0.69	0.98	1.20	0.97	1.07
	ssr1-2C	0.53	1.22	1.11	1.16	1.19
	EMS143	0.41	0.97	0.99	0.94	0.94
	EMS145	0.34	1.13	1.11	0.91	0.94

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