Effects of Trichoderma asperellum Combined Application on Growth and Photosynthesis Characteristics of Populus davidiana×P.alba var. pyramidlis

doi:10.7525/j.issn.1673-5102.2018.01.008

Abstract

Abstract: In order to prepare compound fungi fertilizer of Trichoderma asperellum, we studied the effects of T.asperellum combined application on the growth and photosynthetic characteristics of Populus davidiana×P.alba var. pyramidlis(Shanxin poplar) by applying the concentration(5×10³ cfu·cm^-3 soil) with different combinations of T.asperellum conidia T1(Ta536+Ta4), T2(Ta536+Ta4+Ta492) and T3(Ta536+Ta4+Ta492+Ta650) to roots of the tissue-cultured Shanxin poplar seedlings under field conditions. Analysis of variance showed that the induction time and different treatments had significant effects on height, basal stem, chlorophyll content, chlorophyll a/b value, P_n, Cond, C_i and T_r(P<0.05): the seedling heights, basal stems and dry weights of the treatment groups were higher than those of control group(CK) in different degree, and the effect was T3> T2> T1> CK; the biomass of poplar seedlings of T3, T2 and T1 separately increased 17.99%、14.28% and 10.54% compared with CK at 60 d; at 15 d, chlorophyll contents separately increased 7.79%, 6.91% and 4.17% and chlorophyll a/b values separately increased 7.79%, 5.84% and 4.73% compared with CK. In addition, the net photosynthetic rate(P_n), intercellular CO₂ concentration(C_i), stomatal conductance(Cond) and transpiration rate(T_r) of the treatment were higher than those of CK, and the maximum net photosynthetic rate, apparent quantum efficiency and light saturation point of the treatment groups were higher than those of CK(P<0.05). At the same time, the interaction between the inducing durations and the different treatments levels of T.asperellum also significantly influenced the above indexes(except for P_n and C_i)(P<0.05). In brief, combination of 4 strains had synergistic effects that can enhance the adaptability of these strains to environment, promote growth and improve photosynthesis characteristics, and the T3 group has the biggest influence and the fastest action on the poplar seedlings.

Key words: Populus davidiana×P.alba var. pyramidlis(Shanxin poplar), Trichoderma asperellum, synergistic effect, growth promotion, photosynthesis

CLC Number:

S792.247

LIU Zhao-Ying, JIANG Chuan-Ying, ZHAI Tong-Tong, CHANG Yuan, YAO Zhi-Hong, LIU Zhi-Hua, ZHANG Rong-Shu. Effects of Trichoderma asperellum Combined Application on Growth and Photosynthesis Characteristics of Populus davidiana×P.alba var. pyramidlis[J]. Bulletin of Botanical Research, 2018, 38(1): 64-74.

References

1. 穆红梅,杨重军,何宝玉. 哈茨木霉对三种作物种子萌发及生长的影响[J]. 吉林农业科学,2014,39(5):5-7.Mu H M,Yang C J,He B Y. Impact of Trichoderma Solution on Seed Germination and Growth of Three Crops[J]. Journal of Jilin Agricultural Sciences,2014,39(5):5-7.
2. 尹大川,杨立宾,邓勋,等. 绿木霉对樟子松苗木生长指标及生理生化指标的影响[J]. 北京林业大学学报,2015,37(1):78-83.Yin D C,Yang L B,Deng X,et al. How Trichoderma virens affects growth indicators,physiological and biochemical parameters of Pinus sylvestris var. mongolica seedlings[J]. Journal of Beijing Forestry University,2015,37(1):78-83.
3. 张树武,徐秉良,程玲娟,等. 深绿木霉对白三叶草促生作用及生理生化特性的影响[J]. 草业学报,2015,24(2):161-167.Zhang S W,Xu B L,Cheng L J,et al. Effects of Trichoderma aureoviride fermentation on the growth and physiological characteristics of Trifolium repens[J]. Acta Prataculturae Sinica,2015,24(2):161-167.
4. 陈建爱,王未名,刘益同. 生物复合肥木霉T1010对花生生长的影响[J]. 花生学报,2008,37(3):29-32.Chen J A,Wang W M,Liu Y T. Effect of Trichoderma aureviride T1010 Compound Fertilizer on Growth of Peanut[J]. Journal of Peanut Science,2008,37(3):29-32.
5. Chang Y C,Baker R,et al. Increased growth of plants in the presence of the biological control agent Trichoderma harzianum[J]. Plant Disease,1986,70(2):145-148.
6. 陆宁海,吴利民,田雪亮,等. 哈茨木霉RT-12对黄瓜幼苗促生作用的机理[J]. 江苏农业学报,2007,23(3):254-255.Liu N H,Wu L M,Tian X L,et al. Mechanism of Trichoderma harzianum RT-12 on Cucumber Seedling Growth[J]. Jiangsu Journal of Agricultural Sciences,2007,23(3):254-255.
7. 梁志怀,魏林,郑明福,等. 哈茨木霉发酵产物对豇豆产量和品质的影响[J]. 湖南农业科学,2005(5):38-40,43.Liang Z H,Wei L,Zheng M F,et al. Effects of the Fermentation Filtrate of Trichoderma harzianum on Yield and Quality of Cowpea[J]. Hunan Agricultural Sciences,2005(5):38-40,43.
8. Lorito M,Woo S L,Harman G E,et al. Translational Research on Trichoderma:From 'Omics to the Field[J]. Annual Review of Phytopathology,2010,48(1):395.
9. Yedidia I,Benhamou N,Chet Ⅱ. Induction of Defense Responses in Cucumber Plants(Cucumis sativus L.) by the Biocontrol Agent Trichoderma harzianum[J]. Applied and Environmental Microbiology,1999,65(3):1061-1070.
10. Martínez-Medina A,Del Mar Alguacil M,Pascual J A,et al. Phytohormone Profiles Induced by Trichoderma Isolates Correspond with Their Biocontrol and Plant Growth-Promoting Activity on Melon Plants[J]. Journal of Chemical Ecology,2014,40(7):804-815.
11. Harman Gary. Myths and Dogmas of Biocontrol Changes in Perceptions Derived from Research on Trichoderma harzinum T-22[J]. Plant Disease,2000,84(4):377-393.
12. 张量,张敬泽. 渐绿木霉抑菌物质的分离纯化及其对植物病原菌的抑制作用[J]. 中国农业科学,2015,48(5):882-888.Zhang L,Zhang J Z. Isolation and Purification of Active Compound from Trichoderma viridescens and Its Inhibitory Activities Against Phytopathogens[J]. Scientia Agricultura Sinica,2015,48(5):882-888.
13. 陈方新,齐永霞,戴庆怀,等. 哈茨木霉对几种植物病原真菌的拮抗作用及其抗药性测定[J]. 中国农学通报,2005,21(11):314-317.Chen F X,Qi Y X,Dai Q H,et al. In-lab determination of antagonism against several plant pathogenic fungi and resistance to fungicides of Trichoderma harzianum[J]. Chinese Agricultural Science Bulletin,2005,21(11):314-317.
14. 吕曼曼,刘志华,王慧,等. 棘孢木霉对杨树苗栽培土理化性质及养分的影响[J]. 植物研究,2015,35(2):289-296.Lü M M,Liu Z H,Wang H,et al. Effects of Trichoderma asperellum on the Physical and chemical properties and chemical properties and nutrient components of the pot coil culturing tissue-cultured Populus davidiana×P. bolleana Seedlings[J]. Bulletin of Botanical Research,2015,35(2):289-296.
15. 曹云,常志州,马艳,等. 沼液施用对辣椒疫病的防治效果及对土壤生物学特性的影响[J]. 中国农业科学,2013,46(3):507-516.Cao Y,Chang Z Z,Ma Y,et al. Effects of Application of Anaerobically Digested Slurry on Suppression of Pepper(Capsicum frutescens L.) Blight and Soil Biological Characteristics[J]. Scientia Agricultura Sinica,2013,46(3):507-516.
16. 曾华兰,叶鹏盛,李琼芳,等. 哈茨木霉T23对花生的促生增产作用[J]. 云南农业大学学报,2005,20(1):145-146.Zeng H L,Ye P S,Li Q F,et al. Effects of Trichoderma harzianum T23 on Peanut Yield[J]. Journal of Yunnan Agricultural University,2005,20(1):145-146.
17. 朱国栋,吕曼曼,杨兴堂,等. 木霉Ta650对杨树移栽苗生长及光合特性的影响[J]. 中国农学通报,2015,31(10):7-12.Zhu G D,Lv M M,Yang X T,et al. Effects of Trichoderma asperellum 650 on the growth and leaf photosynthetic characteristics of transplanted poplar seedlings(Populus davidiana×P. alba var. pyramidalis)[J]. Chinese Agricultural Science Bulletin,2015,31(10):7-12.
18. 姜传英,朱国栋,姚志红,等. 3个棘孢木霉菌株对山新杨组培移栽苗生长和光合特性的影响[J]. 草业科学,2016,33(6):1189-1199.Jiang C Y,Zhu G D,Yao Z H,et al. Effects of three Trichoderma asperellumon strains on the growth and photosynthetic characteristics of tissue-cultured Populus davidiana×P. alba var. Pyramidalis seedlings[J]. Pratacultural Science,2016,33(6):1189-1199.
19. 王学奎. 植物生理生化实验原理和技术:第2版[M]. 北京:高等教育出版社,2006:278-279.Wang X K. Principles and Techniques of Plant Physiological Biochemical Experiment:2nd[M]. Beijing:Higher Education Press,2006:278-279.
20. Farquhar G D,Von Caemmerers S,Berry J A. A biochemical model of photosynthetic CO₂ assimilation in leaves of C₃ species[J]. Planta,1980,149(1):78-90.
21. 张广志,杨合同,周红姿,等. 土壤杀菌剂对木霉根际竞争的影响及木霉对玉米生长作用的初步研究[J]. 农业环境科学学报,2007,26(3):229-231.Zhang G Z,Yang H T,Zhou H Z,et al. Competitive Effects of Fungicide on Rhizosphere of Trichoderma spp. in Soil and Maize Growth[J]. Journal of Agro-Environment Science,2007,26(3):229-231.
22. Windham M T,Elad Y,Baker R. A mechanism for increased plant growth induced by Trichoderma spp. [J]. Phytopathology,1986,76(5):518-521.
23. 王艳哲,刘秀位,孙宏勇,等. 水氮调控对冬小麦根冠比和水分利用效率的影响研究[J]. 中国生态农业学报,2013,21(3):282-289.Wang Y Z,Liu X W,Sun H Y,et al. Effects of water and nitrogen on root/shoot ratio and water use efficiency of winter wheat[J]. Chinese Journal of Eco-Agriculture,2013,21(3):282-289.
24. 张敏,胡丽,杨春平,等. 木霉L24菌株对小麦幼苗生长的影响[J]. 安徽农业科学,2010,38(4):1765-1766,1785.Zhang M,Hu L,Yang C P,et al. Effect of Trichoderma Strain L24 on the Growth of Wheat Seedlings[J]. Journal of Anhui Agricultural Sciences,2010,38(4):1765-1766,1785.
25. Jiao L Y,Ding H D,Wang L H,et al. Bisphenol A effects on the chlorophyll contents in soybean at different growth stages[J]. Environmental Pollution,2017,223:426-434.
26. 曹翠玲,李生秀. 供氮水平对小麦生殖生长时期叶片光合速率、NR活性和核酸含量及产量的影响[J]. 植物学报,2003,20(3):319-324.Cao C L,Li S X. Effect of Nitrogen Level on the Photosynthetic Rate,NR Activity and the Contents of Nucleic Acid of Wheat Leaf in the Stage of Reproduction[J]. Chinese Bulletin of Botany,2003,20(3):319-324.
27. Al-Hashmi,Claereboudt K A,Al-Azri M R,et al. Seasonal Changes of Chlorophyll a and Environmental Characteristics in the Sea of Oman[J]. The Open Oceanography Journal,2010,4(1):107-114.
28. Shibghatallah M A H,Khotimah S N,Suhandono S,et al. Measuring leaf chlorophyll concentration from its color:A way in monitoring environment change to plantations[C]. //American Institute of Physics,2013:210-213.
29. 陆宁海,徐瑞富,房振宏,等. 哈茨木霉对小麦和玉米幼苗生长的影响[J]. 江苏农业学报,2005,21(3):238-240.Lu N H,Xu R F,Fang Z H,et al. Influence of T. harzianum on Growth of Wheat and Corn Seedling[J]. Jiangsu Journal of Agricultural Sciences,2005,21(3):238-240.
30. 陈为京,李润芳,杨焕明,等. 黄绿木霉T1010对日光温室番茄功能叶光合色素含量及光系统Ⅱ光化学效率的影响[J]. 中国农学通报,2010,26(9):178-183.Chen W J,Li R F,Yang H M,et al. Effect of Trichoderma aureoviride 1010 on Chlorophyll and Carotenoid Content and F_v/F_m of Tomato Functional Leaves in Solar-Greenhouse[J]. Chinese Agricultural Science Bulletin,2010,26(9):178-183.
31. 冯虎元,安黎哲,王勋陵. 环境条件对植物稳定碳同位素组成的影响[J]. 植物学报,2000,17(4):312-318.Feng H Y,An L Z,Wang X L. A review on effect of environmental factors on stable carbon isotope composition in plants[J]. Chinese Bulletin of Botany,2000,17(4):312-318.
32. Collatz G J. Influence of certain environmental factors on photosynthesis and photorespiration in Simmondsia chinensis[J]. Planta,1977,134(2):127-132.
33. 燕嗣皇,吴石平,陆德清,等. 木霉生防菌对根际微生物的影响与互作[J]. 西南农业学报,2005,18(1):40-46.Yan S H,Wu S P,Lu D Q,et al. Effects of biocontrol strain of Trichoderma harzianum on microflora in rhizosphere and its interactions with microbe[J]. Southwest China Journal of Agricultural Sciences,2005,18(1):40-46.

[1]	Jing ZHANG, Weixi ZHANG, Changjun DING, Zhengsai YUAN, Lirui DAI, Xiaohua SU, Yingbai SHEN, Guanzheng QU. Comparative Analysis of Growth, Photosynthetic Physiology and Root Tip Ion Flow Characteristics of Five Poplar Varieties [J]. Bulletin of Botanical Research, 2024, 44(1): 96-106.
[2]	Tingting LI, Liu YANG, Xiaoxia LI, Yisong WANG, Xiuwei WANG. Different Nitrogen Forms on the Photosynthetic Characteristics and Growth of Fraxinus mandshurica and Quercus mongolica [J]. Bulletin of Botanical Research, 2023, 43(2): 207-217.
[3]	Mengshuo LI, Yingze LIU, Huan LU, Sheng QIANG. Photosynthetic Capacity Differentiation and Gene Transcription in Different Geographical Populations of Arabidopsis thaliana under Common Garden conditions [J]. Bulletin of Botanical Research, 2023, 43(1): 90-99.
[4]	Xiwu DU, Jun QIN, Kang YE, Yonghong HU, Yiwei TAO, Yongzheng PENG, Yanxiang SHEN, Yan LIANG, Li ZENG. Effects of Flooding Stress on Photosynthetic Characteristics of Yulania stellata and Its Cultivars [J]. Bulletin of Botanical Research, 2022, 42(3): 483-491.
[5]	Dong ZHANG, Yan LIU, Han ZHANG, Zi-Jian ZHANG, Yang WANG, Mei-Cen LIU. Response of Photosynthesis and Leaf Morphological Characteristics to Drought Stress in Glycyrrhiza uralensis [J]. Bulletin of Botanical Research, 2021, 41(3): 449-457.
[6]	Fang WANG, Zhi-Min LU, Jun WANG, Shi-Kai ZHANG, Yu-Xi LI, Shao-Chen LI, Jian-Qiu ZHANG, Yu-Chun YANG. Photosynthetic and Stomatal Characteristics of Pinus koraiensis and P.sibirica under Low Temperature Stress [J]. Bulletin of Botanical Research, 2021, 41(2): 205-212.
[7]	DENG Jun-Jie, ABDUL MAJEED BALOCH, HOU Xue-Yue, LI Jia-Zhe, CHA Xin-You, ZHANG Rong-Shu. Effect of Trichoderma harzianum T6 on Rosa chinensis ‘Shi-Jie-Mei’ Growth [J]. Bulletin of Botanical Research, 2020, 40(5): 666-672.
[8]	ZHAO Chun-Jian, LI Yu-Zheng, GUAN Jia-Jing, SU Wei-Ran, TIAN Yao, WANG Ting-Ting, LI Shen, LI Chun-Ying. Effect of Interplanting Taxus cuspidata with Ficus carica on Growth of Two Plants and Activities of Soil Enzymes [J]. Bulletin of Botanical Research, 2020, 40(5): 679-685.
[9]	LIU Ting-Yan, HAO Long-Fei, WANG Qing-Cheng, BAI Shu-Lan. Effects of Different Planting Densities on Cultivating Quality of Padus maackii Seedlings [J]. Bulletin of Botanical Research, 2019, 39(6): 863-868.
[10]	JI Li, HAN Jiao, WANG Fang, WANG Jun, SONG Di, ZHANG Li-Jie, QI Yong-Hui, YANG Yu-Chun. Effects of Drought Stress on Photosynthetic and Physiological Characteristics of Juglans mandshurica Seedlings in Different Soil Substrates [J]. Bulletin of Botanical Research, 2019, 39(5): 722-732.
[11]	LI Zhan-Jun, ZHANG Hou-Liang, GUO Xing, FAN Rui-Hong, MA Ke, LIU Ji-Yun, YANG Feng-Jian. Effects of Different Improvement Measures on Soil Structure and Element Composition,Photosynthesis and Growth of Poplar Leaves in Severe Saline-alkali Soils of Songnen Plain [J]. Bulletin of Botanical Research, 2019, 39(5): 733-739.
[12]	LI Rong-Yu, QIU Guo-Yu, SHEN Xiao-Xue, CHAI Min-Wei. Effects of Ammonium Nitrogen on Physiological and Ecological Characteristics of Kandelia obovata under Cadmium Stress [J]. Bulletin of Botanical Research, 2018, 38(5): 653-660.
[13]	ZHANG Ming, LIU Fu-De, AN Shu-Qing, CAO Xue-Zhang. Leaf Traits of the Saplings of Later Successional Stage Tropical Montane Rain Forest in Hainan Island [J]. Bulletin of Botanical Research, 2017, 37(6): 907-914.
[14]	WANG Ke；DIAO Jian；YANG Rui；HU Yan-Bo；SUN Guang-Yu. Effects of Leaf-spraying Nitrate on Stomatal Dynamics and Photosynthesis of Syringa oblata* Lindl. Leaves [J]. Bulletin of Botanical Research, 2015, 35(6): 843-847.
[15]	ZHANG Qiang1,2；GUO Chuan-You1；ZHANG Xing-Wang1；BAI Kun-Dong2；JIANG De-Bing3. Photosynthesis and Antioxidant Defense Strategies in Overwintering Plants of Tsuga chinensis* and Cyclobalanopsis stewardiana [J]. Bulletin of Botanical Research, 2015, 35(2): 200-207.