Identification of somatic hybrids by the methods of 5S rDNA spacer sequence and RAPD analysis

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Bulletin of Botanical Research ›› 2004, Vol. 24 ›› Issue (1): 120-124.

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Identification of somatic hybrids by the methods of 5S rDNA spacer sequence and RAPD analysis

ZHAO Tong-Jin, XIANG Feng-Ning, NIE Hui, XIA Guang-Min, CHEN Hui-min

Life school of Shandong University, Jinan 250100

Received:2003-02-21 Online:2004-03-15 Published:2016-06-14

Abstract

Abstract: The present paper described the 5S rDNA spacer sequence and RAPD analysis in the identification of hybrid nature of the calli line from interfamilial somatic hybridization between Bupleurum scoronerifolium and Swerita musstii Franch, the calli line from intertribal somatic hybridization between Avena sativa and Triticum aestivum L.and its regenerated plants.We confirmed that they were somatic hybrids

Key words: 5S rDNA, spacer sequence, RAPD, somatic hybrid

ZHAO Tong-Jin, XIANG Feng-Ning, NIE Hui, XIA Guang-Min, CHEN Hui-min. Identification of somatic hybrids by the methods of 5S rDNA spacer sequence and RAPD analysis[J]. Bulletin of Botanical Research, 2004, 24(1): 120-124.

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Identification of somatic hybrids by the methods of 5S rDNA spacer sequence and RAPD analysis

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