Identification of OsBBTIs Gene Family in Rice and Its Expression Analysis under Abiotic Stress

doi:10.7525/j.issn.1673-5102.2025.05.009

Abstract

Abstract:

Bowman-Birk trypsin inhibitor（BBTI） is a widely distributed trypsin inhibitor in plants， which plays an important role in the process of plant resistance to the stresses. In this study， 11 rice BBTIs gene family members were identified at the genome level by bioinformatics methods， among which 10 OsBBTI genes were clustered at the front of chromosome 1， and OsBBTI13 gene was located on chromosome 3. OsBBTIs protein had N-terminal signal peptide and contained 1-3 typical conserved Bowman-Birk type domains. The promoter region of OsBBTIs gene had response elements related to plant hormones jasmonic acid， abscisic acid and the abiotic stresses. Transcriptome data showed that all OsBBTIs gene can be induced by jasmonic acid， and most OsBBTI genes can be expressed in response to the abiotic stresses. qRT-PCR was used to analyze the expression level of OsBBTIs gene under NaHCO₃ and NaCl stresses， the results showed that the expression level of OsBBTIs gene increased under the saline-alkali stresses. This study laid a foundation for further study on the biological function of OsBBTIs proteins in abiotic stress.

Key words: Oryza sativa, OsBBTIs, BowB domain, abiotic stress, expression analysis

CLC Number:

Q786

Yutong LU, Boyan LIU, Renxiang CHE, Yukun ZHANG, Fengjin ZHU, Xingguo LAN. Identification of OsBBTIs Gene Family in Rice and Its Expression Analysis under Abiotic Stress[J]. Bulletin of Botanical Research, 2025, 45(5): 745-754.

Figures/Tables 8

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基因 Gene	上游引物 Forward primer（5′→3′）	下游引物 Reverse primer（5′→3′）
OsBBTI1	CAAAGCAAGCAAGACGTCCA	GATCGGGAGGCGTATGGTG
OsBBTI3	GATTGCTGCGACAACACCAC	GAGTGCATGGTGTGCACTTG
OsBBTI12	TGCGACGACATCGTCAAGAA	TCCTTCACTCAGACAACGGC
Actin	CGTCCTCCTGCTTGTTTCTC	TAGGCCGGTTGAAAACTTTG

基因名称 Gene name	基因号 Gene_ID	基因长度 Gene length/bp	编码序列长度 CDS length/bp	氨基酸数 Amino acid number	分子质量 Molecular mass /Da	等电点 Isoelectric point	亚细胞定位 Subcellular localization
OsBBTI1	Os01g0123900	858	582	193	21 351.29	5.04	extr
OsBBTI2	Os01g0124000	910	561	186	20 100.09	4.90	extr
OsBBTI3	Os01g0124100	930	780	259	27 918.89	5.32	extr
OsBBTI4	Os01g0124200	1 059	756	251	27 734.96	5.36	extr
OsBBTI5	Os01g0124401	1 063	765	254	27 789.74	5.38	extr
OsBBTI6	Os01g0124600	573	573	190	20 735.18	4.46	extr
OsBBTI7	Os01g0124650	847	558	185	20 103.10	8.49	extr
OsBBTI8	Os01g0127600	878	540	179	19 334.10	8.08	extr
OsBBTI11	Os01g0131900	919	306	101	10 770.82	8.84	extr
OsBBTI12	Os01g0132000	756	303	100	10 716.85	8.70	extr
OsBBTI13	Os03g0823400	979	384	127	13 481.52	8.94	chlo