Molecular Phylogenetic Analysis of Cerasus Plants in China

doi:10.7525/j.issn.1673-5102.2020.06.010

Abstract

Abstract:

To provide some molecular basis for the species classification and lineage location of cherry blossoms mainly planted in China， with 88 cherry blossoms， by nuclear gene ITS and chloroplast intergenic sequence， trnH-psbA were sequenced， and after processed by software MEGA 6， phylogenetic tree was constructed. The phylogenetic development was confused in Cerasus serrulata group， which is related to the complex genetic background and the close phylogenetic relationship among the primitive parental， meanwhile， it could be clustered together in most of the cultivars C.subhirtella group or C.campanulata group， and could be separated from the cultivars of the Cerasus serrulata group， however， it was difficult to distinguish in many hybrids using ITS and trnH-psbA， which indicated that DNA barcoding technology was difficult to apply to the phylogenetic relationship of hybrids， in addition， some cherry cultivars could be identified by ITS or trnH-psbA sequence length， and some unidentified varieties were also revised.

Key words: Cerasus, ITS, chloroplast sequence, phylogenetic, species identification

CLC Number:

Q341

Tao FU, Zhi-Long WANG, Le-Jing LIN, Li LIN, Wen LI, Dong-Ming YUAN. Molecular Phylogenetic Analysis of Cerasus Plants in China[J]. Bulletin of Botanical Research, 2020, 40(6): 876-885.

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