Effects of H2S and PLDα1 on Drought Stress in Arabidopsis thaliana

doi:10.7525/j.issn.1673-5102.2018.03.013

Abstract

Abstract: The experiment was conducted to study the role of phospholipase Dα1(PLDα1) and the gas signal hydrogen sulfide(H₂S) in response to drought stress and the possible signal relationship. The wild type Arabidopsis thaliana(WT), PLDα1 mutant(pldα1-1) and the LCD mutant plant(lcd-4) was used as experiment materials, and 0.3 mol·L^-1 mannitol was used to simulate drought stress. The content of endogenous H₂S, the activities of PLD, L/D-CDes, and the relative expression of PLDα1, LCD and DCD in wild type Arabidopsis were significantly changed under drought stress. Compared with WT, pldα1-1 and lcd-4 were more sensitive to drought condition. Application of NaHS could promote the seed germination and endogenous H₂S production in WT, pldα1-1 and lcd-4. Exogenous addition of PA could increase the germination rate and endogenous H₂S concentration in WT and pldα1-1, but had no significant effect in lcd-4. The signal molecules H₂S and PLDα1 played important roles in the plant response to drought stress, and H₂S might be located at downstream of PLDα1 in signal transduction process to regulate the seed germination of A. thaliana.

Key words: Arabidopsis thaliana, drought stress, hydrogen sulfide, phospholipase Dα1

CLC Number:

Q949.748.3

ZHAO Min, YANG Ning, CHEN Lu, AN Yan-Huang, LI Wen-Ling, ZHAO Feng-Feng. Effects of H₂S and PLDα1 on Drought Stress in Arabidopsis thaliana[J]. Bulletin of Botanical Research, 2018, 38(3): 406-414.

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Effects of H₂S and PLDα1 on Drought Stress in Arabidopsis thaliana

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