氟喹诺酮类抗生素胁迫下翅碱蓬生理响应与分子调控特征

doi:10.7525/j.issn.1673-5102.2026.04.012

摘要/Abstract

摘要：

氟喹诺酮类抗生素（fluoroquinolone antibiotics，FQs）广泛存在于农田灌溉水、畜禽养殖废水及滨海湿地中，对植物生长与生态过程构成潜在胁迫。以氧氟沙星（ofloxacin，OFX）和恩诺沙星（enrofloxacin，ENR）为代表，通过生理生化指标测定与转录组分析，系统解析翅碱蓬（Suaeda salsa）在氟喹诺酮类抗生素胁迫下的生理响应特征及分子调控机制。结果表明：OFX与ENR均对翅碱蓬地上部和地下部造成显著氧化胁迫，其中ENR胁迫下膜脂过氧化程度更为严重，并伴随苯丙烷代谢及ABC转运体（ATP-binding cassette transporter）相关基因的显著差异表达；OFX胁迫则主要影响光系统结构与功能，通过WRKY转录因子家族（WRKY transcription factor family）和MAPK（mitogen-activated protein kinase）信号通路相关基因的调控增强抗氧化防御能力。2种抗生素处理均增强糖酵解-丙酮酸代谢途径，为抗胁迫反应提供能量与还原力支持。翅碱蓬在氟喹诺酮类抗生素胁迫下表现出由结构防御、代谢重编程与信号调控协同驱动的多层次适应性响应模式。

关键词: 翅碱蓬, 氟喹诺酮类抗生素, 苯丙烷类代谢, 抗氧化系统, 植物修复

Abstract:

Fluoroquinolone antibiotics（FQs） are widely detected in agricultural irrigation water， livestock wastewater， and coastal wetlands， posing potential stress to plant growth and ecological processes. Taking ofloxacin（OFX） and enrofloxacin（ENR） as representative compounds， this study employed physiological and biochemical assays in combination with transcriptomic analysis to systematically elucidate the physiological responses and molecular regulatory mechanisms of Suaeda salsa under FQs stress. The results showed that both OFX and ENR induced significant oxidative stress in both the aboveground and belowground parts of S. salsa. Comparatively， ENR caused more pronounced lipid peroxidation， which was accompanied by the significant differential expression of genes related to phenylpropanoid metabolism and ABC（ATP-binding cassette） transporters. In contrast， OFX stress primarily impaired photosystem structure and function， while simultaneously enhancing antioxidant defense through the regulation of the WRKY transcription factor family and MAPK（mitogen-activated protein kinase） signaling pathways. Furthermore， both antibiotic treatments promoted the glycolysis-pyruvate metabolism pathway， thereby providing sustained energy supply and reducing power to support stress resistance. Collectively， under FQs stress， S. salsa exhibited a multi-level adaptive response strategy driven by the coordinated regulation of structural defense， metabolic reprogramming， and signaling pathways.

Key words: Suaeda salsa, fluoroquinolone antibiotics, phenylpropanoid metabolism, antioxidant system, phytoremediation

中图分类号:

Q948.1

关沛泽, 匡珮菁, 魏裕庭, 关梓涵, 赵可, 崔玉波. 氟喹诺酮类抗生素胁迫下翅碱蓬生理响应与分子调控特征[J]. 植物研究, 2026, 46(4): 712-727.

Peize GUAN, Peijing KUANG, Yuting WEI, Zihan GUAN, Ke ZHAO, Yubo CUI. Physiological Responses and Molecular Regulatory Characteristics of Suaeda salsa under Fluoroquinolone Antibiotic Stress[J]. Bulletin of Botanical Research, 2026, 46(4): 712-727.

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时间 Time/min	流动相A百分比 Percentage of mobile phase A/%	流动相B百分比 Percentage of mobile phase B/%
（0~1.0］	98	2
（1.0~2.0］	98→2	2→98
（2.0~3.5］	2	98
（3.5~4.0］	2→98	98→2
（4.0~6.0］	98	2

分类 Category	基因 Gene	连接通路 Linked pathway	功能/作用方向 Function/direction of effect
ENR	PAL	Phenylpropanoid biosynthesis	木质素合成/防御反应
ENR	4CL	Phenylpropanoid biosynthesis	细胞壁增强
ENR	CYP450	Sulfur metabolism / Secondary metabolism	解毒与代谢调节
ENR	ABC transporter	Transport / Detoxification	外排抗生素
ENR	CAD	Phenylpropanoid biosynthesis	细胞壁加固/木质素合成
OFX	LHCA1	Photosystem I	光捕获、光系统功能
OFX	LHCB2	Photosystem Ⅱ antenna proteins	光能传递
OFX	PsbO	Photosystem Ⅱ	光系统稳定
OFX	WRKY TF	MAPK signaling / Plant-pathogen interaction	信号转导
OFX	MAPK	MAPK signaling pathway	逆境响应信号
COMMON	SOD	Glutathione metabolism / ROS scavenging	清除ROS
COMMON	CAT	Peroxisome / ROS scavenging	分解H₂O₂
COMMON	GST	Glutathione metabolism	抗氧化与解毒
COMMON	HSP	Protein processing in ER	抗逆保护
COMMON	ALDH	Pyruvate metabolism	碳流分配与能量供给