外源绿原酸通过调控类黄酮代谢增强林下黄芩对叶斑病抗病性

doi:10.7525/j.issn.1673-5102.2026.04.014

摘要/Abstract

摘要：

植物在病原菌胁迫下通常积累多种次生代谢产物，这些代谢物与植物抗逆性密切相关。其中，绿原酸（chlorogenic acid，CGA）可以通过调控次生代谢增强植物抗病性，但相关机制仍缺乏系统研究。为进一步解析其机制，本研究对外源绿原酸处理的黄芩（Scutellaria baicalensis）进行病原菌侵染，测定叶片叶绿素、丙二醛含量及侵染面积和黄酮类物质含量等相关指标，并进行转录组测序分析及酶基因顺式作用元件分析。结果表明：绿原酸显著提高黄芩对叶斑病的抗病性，并影响黄酮类化合物柚皮素和芹菜素的积累。与对照相比，绿原酸处理组黄芩叶片病斑面积较小，叶绿素含量较高，丙二醛含量较低。对绿原酸处理后的样品进行转录组测序分析，共鉴定出563个差异表达基因，主要富集在苯丙烷生物合成、淀粉和蔗糖代谢、亚麻酸代谢、半乳糖代谢、类黄酮生物合成等代谢途径中；其中SbPAL、SbC4H、Sb4CL1、Sb4CL2、SbFNS、SbHCT1、SbHCT2等黄酮合成关键酶基因表达显著上调；顺式作用元件分析表明，这些基因启动子区域富含bHLH、MYB、GRF等转录因子结合位点。综上，本研究表明：外源绿原酸可能通过促进类黄酮生物合成途径关键基因表达，提高黄芩中类黄酮化合物积累，从而增强其对叶斑病的抗病性。

关键词: 绿原酸, 黄芩, 黄酮类化合物, 真菌病害

Abstract:

Chlorogenic acid（CGA） plays an important role in regulating plant disease resistance. Under pathogen stress， plants typically accumulate various secondary metabolites that are closely associated with stress tolerance. Among these， CGA can enhance plant disease resistance by modulating secondary metabolism； however， the underlying mechanisms remain poorly understood. To further elucidate these mechanisms， Scutellaria baicalensis plants treated with exogenous CGA were subjected to pathogen infection. Leaf chlorophyll content， malondialdehyde（MDA） content， lesion area， and flavonoid content were determined， and transcriptome sequencing coupled with cis-acting element analysis of enzyme-coding genes were performed.The results showed that CGA significantly enhanced the resistance of S. baicalensis to leaf spot disease and influenced the accumulation of the flavonoids naringenin and apigenin. Compared with the control， CGA-treated plants exhibited smaller lesion area， higher chlorophyll level， and lower MDA content. Transcriptome analysis of CGA-treated samples identified 563 differentially expressed genes（DEGs）， which were mainly enriched in pathways including phenylpropanoid biosynthesis， starch and sucrose metabolism， linoleic acid metabolism， galactose metabolism， and flavonoid biosynthesis. Notably， key flavonoid biosynthetic enzyme genes-SbPAL， SbC4H， Sb4CL1， Sb4CL2， SbFNS， SbHCT1， and SbHCT2-were significantly upregulated. Cis-acting element analysis further revealed that the promoter regions of these genes were enriched in binding sites for transcription factors such as bHLH， MYB， and GRF.In conclusion， this study demonstrates that exogenous CGA likely enhances the resistance of S. baicalensis to leaf spot disease by promoting the expression of key genes in the flavonoid biosynthesis pathway， thereby increasing flavonoid accumulation.

Key words: chlorogenic acid, scutellaria baicalensis, flavonoid biosynthesis, fungal disease

中图分类号:

S763

薛聪慧, 牛丽丽, 宋治华, 董碧莹, 杨清, 付玉杰, 孟冬. 外源绿原酸通过调控类黄酮代谢增强林下黄芩对叶斑病抗病性[J]. 植物研究, 2026, 46(4): 740-750.

Conghui XUE, Lili NIU, Zhihua SONG, Biying DONG, Qing YANG, Yujie FU, Dong MENG. Exogenous Chlorogenic Acid Enhances Leaf Spot Disease Resistance in Understory Scutellaria baicalensis by Regulating Flavonoid Metabolism[J]. Bulletin of Botanical Research, 2026, 46(4): 740-750.

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