沙枣果总黄酮类化合物的富集研究

doi:10.7525/j.issn.1673-5102.2018.03.020

摘要/Abstract

摘要： 通过对9种型号大孔吸附树脂静态筛选，最终确定AB-8型大孔吸附树脂用于沙枣果总黄酮化合物的富集，并通过动态实验，确定沙枣果总黄酮最优富集条件为：样品浓度2.43 mg·mL^-1，上样速率2 BV·h^-1，乙醇浓度70%，洗脱速率2 BV·h^-1。在最优工艺条件下，沙枣果总黄酮浓度由9.73%增加到49.52%；其中金丝桃苷含量由0.53%增加到3.07%，提高5.79倍。本研究为沙枣果总黄酮的开发利用提供了理论依据。

关键词: 沙枣, 黄酮, 富集研究

Abstract: Based on the static screening experiments, AB-8 resin was determined to be the best resin for enriching flavonoid of Elaeagnus angustifolia from nine kinds of macro-porous resins. According to the dynamic experiment results, some enriching conditions were optimized, as follows:the sample concentration was 2.43 mg·mL^-1; the flow rate was 2 BV·h^-1; the ethanol concentration was 70%; the elution rate was 2 BV·h^-1. The purity of total flavonoids from E. angustifolia was increased by 5.09 times, from 9.73% to 49.52%. The purity of Hypericin was increased by 5.79 times, from 0.53% to 3.07%. The results of this research would provide the theoretical basis and data support for the development and utilization of total flavonoids in E. angustifolia.

Key words: Elaeagnus angustifolia, flavonoids, enrichment

中图分类号:

S143.8

张子东, 张圆圆, 孟永斌, 徐蕾, 贾楠, 项凤影, 路祺. 沙枣果总黄酮类化合物的富集研究[J]. 植物研究, 2018, 38(3): 467-474.

ZHANG Zi-Dong, ZHANG Yuan-Yuan, MENG Yong-Bin, XU Lei, JIA Nan, XIANG Feng-Ying, LU Qi. Flavonoids Enrichment from Elaeagnus angustifolia Fruit[J]. Bulletin of Botanical Research, 2018, 38(3): 467-474.

参考文献

1. 麦合苏木·艾克木,买尔旦·马合木提.新疆沙枣提取物对实验性小鼠腹泻以及小肠推进功能的影响[J].新疆医学,2007,37(3):1-3. Ai Kemu M,Mahemuti M.Effect of promoting function on experimental diarrhea in mice and intestinal extracts from Elaeagnus angustifolia of Xinjiang[J].Xinjiang Journal of Traditional Chinese Medicine,2007,37(3):1-3.
2. 董世林.植物资源学[M].哈尔滨:东北林业大学出版社,1994:176-186. Dong S L.Plant resources[M].Harbin:Northeast Forestry University Press,1994:176-186.
3. 张彦文.查尔酮类化合物的药理作用和构效关系[J].国外医学:药学分册,1996,23(4):218-223. Zhang Y W.Pharmacological effects and structure-activity relationship of chalcone compounds[J].Foreign Medical Sciences Section on Pharmacy,1996,23(4):218-223.
4. 杜崇民,刘春宇.黄酮类化合物抗肿瘤研究进展[J].中国野生植物资源,2007,26(3):4-7. Du C M,Liu C Y.Research advance on anti-tumor actions of flavones[J].Chinese Wild Plant Resources,2007,26(3):4-7.
5. 鄢春旻.黄酮类化合物抗病毒及抗炎活性研究[D].南京:南京大学,2012. Yan C M.Antiviral and anti-inflammatory activities of flavonoids[D].Nanjing:Nanjing University,2012.
6. 兰真,曾凡骏,曾里,等.生物类黄酮对心血管系统的调节及作用机理[J].现代预防医学,2005,32(6):613-615. Lan Z,Zeng F J,Zeng L,et al.Adjustive effects and mechanism of bioflavonoids on heart and blood vessel system[J].Modern Preventive Medicine,2005,32(6):613-615.
7. 张逸凡,于庆海.淫羊藿总黄酮的免疫调节作用[J].沈阳药科大学学报,1999,16(3):182-184. Zhang Y F,Yu Q H.The immunoregulatory effect of total flavones epimedium[J].Journal of Shenyang Pharmaceutical University,1999,16(3):182-184.
8. 吴家安,安图,张磊,等.中草药抗HIV活性:使用和发展潜力[J].中国药物化学,2001,29(1):69-81. Wu J A,An T,Zhang L,et al.Anti-HIV activity of Chinese herbs:usage and development potential[J].Chinese Pharmaceutical Chemistry,2001,29(1):69-81.
9. 于长青,张东杰,牟光庆,等.麦胚黄酮类提取物对血脂和抗氧化的影响[J].营养学报,2001,23(4):390-392. Yu C Q,Zhang D J,Mu G Q,et al.Effect of flavonoids extract of wheat germ on serum lipids and antioxidation[J].Acta Nutrimenta Sinica,2001,23(4):390-392.
10. 尉芹,王冬梅,马希汉,等.杜仲叶总黄酮含量测定方法研究[J].西北农林科技大学学报:自然科学版,2001,29(5):119-123. Wei Q,Wang D M,Ma X H,et al.A study on the measurement of flavonoids in the leaves of Eucommia ulmoides[J].Journal of Northwest Sci-Tech University of Agriculture and Forestry:Natural Science Edition,2001,29(5):119-123.
11. 贾志申,唐梦成,吴建明.桑叶中黄酮类化合物的含量测定及其对超氧阴离子自由基的清除作用[J].食品化学,1999,64(4):555-559. Jia Z S,Tang M C,Wu J M.The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals[J].Food Chemistry,1999,64(4):555-559.
12. 池汝安,周芳,黄琨,等.S-8型大孔树脂葛根素的吸附研究[J].中国天然药物,2011,9(2):120-125. Chi R A,Zhou F,Huang K,et al.Adsorption behaviors of puerarin on S-8 macroporous resin[J].Chinese Journal of Natural Medicines,2011,9(2):120-125.
13. Jia G T,Lu X Y.Enrichment and purification of madecassoside and asiaticoside from Centella asiatica extracts with macroporous resins[J].Journal of Chromatography A,2008,1193(1-3):136-141.
14. 朱思佳,吕秀阳,何世伟,等.大孔树脂对甘油的静态吸附及其热力学研究[J].化工时刊,2006,20(11):5-7,32. Zhu S J,Lü X Y,He S W,et al.Study on the static adsorption behaviors and thermodynamic properties of glycerine on macroporous resins[J].Chemical Industry Times,2006,20(11):5-7,32.
15. 李云志.元宝枫叶黄酮提取、纯化、分离与结构鉴定的研究[D].成都:四川大学,2005:30-40. Li Y Z.Extraction,purification,separation and identification of flavonoids in the leaves of Acer truncatum[D].Chengdu:Sichuan University,2005:30-40.
16. 陶海腾,陈晓明,吕飞杰,等.大孔吸附树脂法纯化木薯叶黄酮的初步研究[J].食品工业科技,2009,30(8):192-194. Tao H T,Chen X M,Lü F J,et al.Primary study on macroporous resin for purification of flavonoids in cassava leaves[J].Science and Technology of Food Industry,2009,30(8):192-194.
17. 范婧娴,刘友平,李旻,等.大孔吸附树脂纯化干姜姜酚类成分工艺研究[J].时珍国医国药,2009,20(11):2818-2820. Fan J X,Liu Y P,Li M,et al.Purification of gingerols from zingibers with macroporous resin[J].Lishizhen Medicine and Materia Medica Research,2009,20(11):2818-2820.
18. 吴晓,周礼明,马威,等.大孔树脂纯化沙苑子总黄酮工艺优选[J].辽宁中医药大学学报,2015,17(1):54-57. Wu X,Zhou L M,Ma W,et al.Optimization of purification technology for total flavonoids from semen astragali complanati by macroporous adsorption resin[J].Journal of Liaoning University of Traditional Chinese Medicine,2015,17(1):54-57.
19. 金继曙,都述虎,种明才.用大孔吸附树脂分离白芍总甙[J].中国中药杂志,1994,19(1):31. Jin J S,Du S H,Zhong M C.Separation of total peony from Daphnia majus using macroporous adsorption resin[J].China Journal of Chinese Materia Medica,1994,19(1):31.
20. 安彩贤,李冶姗.大孔吸附树脂分离纯化葛根与山楂叶中总黄酮的研究[J].中成药,2004,26(9):698-701. An C X,Li Y S.Separation and purification of total flavone from Kudzuvine root and hawthorn leaves by macroporous resin[J].Chinese Traditional Patent Medicine,2004,26(9):698-701.
21. 任顺成,丁霄霖.玉米须中总黄酮的提取及树脂精制[J].中草药,2003,34(12):1092-1094. Ren S C,Ding X L.Extraction of total flavonoids in corn silk and resin refining[J].Chinese Traditional and Herbal Drugs,2003,34(12):1092-1094.
22. 胡晖.翻白草总黄酮微波辅助提取与纯化工艺[J].广东农业科学,2010,37(6):145-147. Hu H.Microwave-assisted extraction and purification of total flavonoids in Potentilla discolor Bunge[J].Guangdong Agricultural Sciences,2010,37(6):145-147.
23. 孙素琴,周群,陈建波.中药红外光谱分析与鉴定[M].北京:化学工业出版社,2010:4. Sun S Q,Zhou Q,Chen J B.Analysis of traditional Chinese medicine by infrared spectroscopy[M].Beijing:Chemical Industry Press,2010:4.
24. Sun S Q,Chen J B,Zhou Q,et al.Application of mid-infrared spectroscopy in the quality control of traditional Chinese medicines[J].Planta Medica,2010,76(17):1987-1996.
25. 李志勇,王朝鲁,孙素琴,等.蒙药草乌叶及其提取物化学成分的红外光谱分析[J].中国中药杂志,2011,36(23):3281-3285. Li Z Y,Wang C L,Sun S Q,et al.Analysis of chemical components in Aconitum kusnezoffii leaves and their extracts by infrared spectroscopy[J].China Journal of Chinese Materia Medica,2011,36(23):3281-3285.
26. Zhang Y L,Chen J B,Lei Y,et al.Evaluation of different grades of ginseng using Fourier-transform infrared and two-dimensional infrared correlation spectroscopy[J].Journal of Molecular Structure,2010,974(1-3):94-102.
27. 沈秋仙.槲蕨根茎黄酮类物质提取条件的优化[J].丽水学院学报,2005,27(5):64-66. Shen Q X.Optimization of extracting condition of flavonoids from rhizoma of drynaria rhizoma drynariae[J].Journal of Lishui University,2005,27(5):64-66.
28. 马涛,毛红燕,石太渊,等.辽五味子黄酮纯化及定性光谱分析[J].食品与生物技术学报,2012,31(1):101-105. Ma T,Mao H Y,Shi T Y,et al.Separation purification and spectrum of flavonoids from Liao Schisandra chinensis[J].Journal of Food Science and Biotechnology,2012,31(1):101-105.
29. 赵璇,金素娟,李占军,等.紫心甘薯的利用价值与开发前景[J].河北农业科学,2012,16(5):84-86. Zhao X,Jin S J,Li Z J,et al.Utility value and development prospect of purple-fleshed sweet potato[J].Journal of Hebei Agricultural Sciences,2012,16(5):84-86.

[1]	李楠, 田小霞, 毛培春, 郑明利, 孟林, 云岚. 马蔺花器官表型特征及色素分析[J]. 植物研究, 2024, 44(1): 51-61.
[2]	李浙浙, 张一丹, 王波, 王郑昊, 王璐, 程蓓蓓. 木槿开花过程中花色素的变化规律及其影响因素[J]. 植物研究, 2023, 43(4): 550-561.
[3]	李娜, 王潇楠. 拟南芥种皮色素形成突变体的筛选与表型鉴定[J]. 植物研究, 2023, 43(1): 59-68.
[4]	郭梦桥, 陈轩宇, 洪森荣, 李娇, 樊洁, 程薪宇. 三叶青叶表型多样性与地上部分总黄酮含量相关性[J]. 植物研究, 2022, 42(5): 876-885.
[5]	苗永美, 童元, 方达, 王宇豪. 稀土镧对铁皮石斛不定芽诱导、植株生长及次生代谢产物合成的影响[J]. 植物研究, 2020, 40(6): 839-845.
[6]	赵历强, 单春苗, 张声祥, 施圆圆, 马克龙, 吴家文. 基于转录组测序的细风轮花青素合成途径及关键酶基因分析[J]. 植物研究, 2020, 40(6): 886-896.
[7]	李辛雷, 王佳童, 孙振元, 王洁, 殷恒福, 范正琪, 李纪元. 崇左金花茶花朵和叶片类黄酮UPLC-Q-TOF-MS分析[J]. 植物研究, 2019, 39(3): 365-371.
[8]	徐夙侠, 黄青云, 林春松, 徐一丁, 李紫阳, 刘育梅, 黄一锦. 不同育性三角梅品种之间的形态、花粉活力、造访昆虫及黄酮醇含量的比较研究[J]. 植物研究, 2018, 38(6): 860-868.
[9]	王荣, 成梦琳, 刘慧娜, 赵大球, 陶俊. 黑暗条件下褪黑素对栀子叶片类黄酮含量及相关基因表达水平的影响[J]. 植物研究, 2018, 38(4): 559-567.
[10]	张圆圆, 孟永斌, 徐蕾, 贾楠, 孟庆焕, 项凤影, 路祺. 匀浆法提取沙枣果总黄酮工艺研究[J]. 植物研究, 2018, 38(4): 625-631.
[11]	于婷婷, 倪秀珍, 高立宏, 韩国军, 朱长甫, 盛彦敏. 高等植物二氢黄酮醇4-还原酶基因研究进展[J]. 植物研究, 2018, 38(4): 632-640.
[12]	张强, 刘一, 吴晓敏, 束良佐, 宋运贤, 陈楚, 龙民慧. 两种氮素形态对药用植物三叶青中黄酮类活性成分积累的影响及相关分子机制的初步探讨[J]. 植物研究, 2018, 38(3): 367-376.
[13]	杨楠, 王曦, 郭晓瑞, 刘洋, 唐中华, 王洪政. 黄芪种子萌发及萌发后生长过程中黄酮类化合物合成的动态变化[J]. 植物研究, 2018, 38(2): 298-305.
[14]	罗猛, 乔琪, 牟璠松, 阮鑫, 杨璇, 邢文淼, 杨雨春, 祁永会, 段喜华. 胡桃楸活性成分时空动态及抗肿瘤活性初步研究[J]. 植物研究, 2017, 37(6): 947-952.
[15]	徐夙侠, 黄青云, 林春松, 黄一锦, 胡欧. 三色(Bougainvillea peruviana‘Thimma’)在转录水平的甜菜色素和类黄酮积累比较[J]. 植物研究, 2017, 37(2): 249-258.