植物研究 ›› 2025, Vol. 45 ›› Issue (3): 447-459.doi: 10.7525/j.issn.1673-5102.2025.03.014

• 研究论文 • 上一篇    

不同菌根类型树种与桉树混交对土壤微生物群落的影响

王亚鑫1,2, 朱媛1, 孟森1, 明安刚3, 贾宏炎3, 覃方锉1(), 陆俊锟1   

  1. 1.中国林业科学研究院热带林业研究所,广州 510520
    2.南京林业大学风景园林学院,南京 210037
    3.中国林业科学研究院热带林业实验中心,广西友谊关森林生态系统国家定位观测研究站,凭祥 532600
  • 收稿日期:2024-12-28 出版日期:2025-05-20 发布日期:2025-05-23
  • 通讯作者: 覃方锉 E-mail:qinfc@caf.ac.cn
  • 作者简介:王亚鑫(1999—),女,硕士研究生,主要从事林木微生物资源利用研究。
  • 基金资助:
    中国林业科学研究院基本科研业务费项目(CAFYBB2023MA007);国家自然科学基金面上项目(3247141238);国家重点研发计划课题(2023YFD2201005);广东省基础与应用基础研究基金项目(2022A1515010413)

The Effects of Mixed Eucalyptus Plantations with Different Mycorrhizal Tree Species on Soil Microbial Community

Yaxin WANG1,2, Yuan ZHU1, Sen MENG1, Angang MING3, Hongyan JIA3, Fangcuo QIN1(), Junkun LU1   

  1. 1.Research Institute of Tropical Forestry,Chinese Academy of Forestry,Guangzhou 510520
    2.College of Landscape Architecture,Nanjing Forestry University,Nanjing 210037
    3.Experimental Center of Tropical Forestry,Chinese Academy of Forestry,Guangxi Youyiguan Forest Ecosystem Research Station,Pingxiang 532600
  • Received:2024-12-28 Online:2025-05-20 Published:2025-05-23
  • Contact: Fangcuo QIN E-mail:qinfc@caf.ac.cn

摘要:

桉树混交林在促进土壤养分循环、提高生产力和生态系统功能等方面有重要作用。菌根共生是影响混交林种间互作关系的重要因素。然而,不同菌根类型的混交树种对桉树人工林土壤微生物群落结构及功能的影响尚缺乏研究。该研究以桉树纯林及其与降香黄檀(Dalbergia odorifera,固氮-丛枝菌根树种)、火力楠(Michelia macclurei,丛枝菌根树种)、麻栎(Quercus acutissima,外生菌根树种)的混交林为研究对象,利用宏基因组测序技术,探究4种不同林型土壤微生物群落多样性、结构功能、细菌-真菌共现网络模式差异及其与土壤养分的关系。结果表明:混交林显著提高土壤微生物群落物种丰富度,改变微生物功能,增加微生物共现网络复杂性。降香黄檀与桉树混交林土壤微生物丰富度、细菌群落好氧化能异养和发酵功能基因相对丰度、真菌-细菌微生物共现网络模块度均最高;土壤细菌、真菌群落结构与其他3种林型差异显著。火力楠与桉树、麻栎与桉树混交林土壤细菌、真菌的碳氢化合物降解、氮固定、甲烷氧化功能基因相对丰度均高于桉树纯林。外生菌根树种(麻栎)与桉树混交林土壤微生物共现网络边数、平均度及网络稳定性最高,其核心微生物丰度与NO3-水平显著相关。丛枝菌根树种(降香黄檀、火力楠)与桉树混交林土壤核心微生物丰度与NH4+水平显著相关。

关键词: 桉树, 混交林, 菌根类型, 土壤微生物, 共现网络

Abstract:

Mixed-species plantations can promote soil nutrient cycling, productivity, and ecosystem functions. Mycorrhizal associations play important roles in affecting species coexistence and nutrient cycling in mixed plantations. However, our understanding on the effects of mixed tree species with different mycorrhizal types on soil microbial community associated with soil nutrients of Eucalyptus plantations remains limited. In this study, we conducted metagenomic sequencing to investigate the changes in soil microbial community structure and functions, co-occurrence networks between bacteria and fungi, and their relationships with soil nutrients for one monospecific Eucalyptus plantation and three mixed Eucalyptus plantations with different mycorrhizal tree species, including Dalbergia odorifera(arbuscular mycorrhizal and N2-fixing, AM-NF), Michelia macclurei(arbuscular mycorrhizal, AM), and Quercus acutissima(ectomycorrhizal, ECM). The results indicated that the mixed stands significantly increased the richness of soil microbial community, enhanced the abundance of genes related to soil nutrient cycling, and increased the complexity of microbial co-occurrence networks. The mixed plantations of Eucalyptus with D. odorifera had the highest soil microbial richness, relative abundance of bacterial community associated with aerobic chemoheterotrophy and fermentation, and the modularity of fungal-bacterial co-occurrence network; meanwhile, either the bacterial or fungal communities were significantly different from the other three plantations. The mixed Eucalyptus plantations with M. macclurei or Q. acutissima species had the highest abundance of microbial functional genes such as hydrocarbon degradation, nitrogen fixation, and methanotrophy. In addition, the microbial co-occurrence networks in the mixed Eucalyptus plantations with Q. acutissima have the highest number of edges, average degree, and network stability. The richness of key microbes in co-occurrence networks was closely correlated with soil NO3- level. However, the richness of microbial community of the networks in the mixed Eucalyptus plantations with D. odorifera and M. macclurei was largely associated with soil NH4+ level.

Key words: Eucalyptus, mixed-species plantations, mycorrhizal type, soil microbial community, co-occurrence network

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