不同测定方法下叶片穿透力与叶片功能性状之间的关系

doi:10.7525/j.issn.1673-5102.2023.06.013

植物研究 ›› 2023, Vol. 43 ›› Issue (6): 910-922.doi: 10.7525/j.issn.1673-5102.2023.06.013

不同测定方法下叶片穿透力与叶片功能性状之间的关系

欧美景¹^,², 李红燕¹^,², 赵秋菊¹^,², 李佳蔚¹^,²()

^1.广西大学林学院，广西森林生态与保育重点实验室，南宁 530004
^2.广西大学林学院，亚热带农业生物资源保护与利用国家重点实验室，南宁 530004

收稿日期:2022-11-30 出版日期:2023-11-20 发布日期:2023-11-08
通讯作者: 李佳蔚 E-mail:lijiawei1662020@163.com
作者简介:欧美景（1998-），女，硕士研究生，主要从事植物生理生态研究。
基金资助:
国家自然科学基金(31901092);广西自然科学基金(2021GXNSFBA075059)

Relationship Between Leaf Force-to-punch and Leaf Functional Traits under Different Measurement Methods

Meijing OU¹^,², Hongyan LI¹^,², Qiuju ZHAO¹^,², Jiawei LI¹^,²()

^1.Guangxi Key Laboratory of Forest Ecology and Conservation，College of Forestry，Guangxi University，Nanning 530004
^2.State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources，College of Forestry，Guangxi University，Nanning 530004

Received:2022-11-30 Online:2023-11-20 Published:2023-11-08
Contact: Jiawei LI E-mail:lijiawei1662020@163.com
About author:E-mail：lijiawei1662020@163.com
OU Meijing（1998—），female，master candidate，major in plant physiology and ecology.
Supported by:
the National Natural Science Foundation of China(31901092);Natural Science Foundation of Guangxi(2021GXNSFBA075059)

摘要/Abstract

摘要：

为探究不同校准方法和不同穿刺针直径作用下叶片穿透力测定结果是否存在差异，以及该结果与叶片功能性状之间的关系，选取了30种双子叶植物，分别测定其2.0、1.0、0.5 mm穿刺针直径下的穿透力、撕裂力和叶片的功能性状等指标。结果表明：（1）叶片穿透力在3种不同穿刺针直径作用下差异显著（P<0.05），其在以周长校准方法下，同一物种在穿刺针直径0.5与1.0 mm的差异显著性占所有物种之比为76%、0.5 mm与2.0 mm为76%、1.0 mm与2.0 mm为33%（P<0.05）；而同样在以横截面积校准下则为66%、56%、30%（P<0.05）。（2）不同校准方法下3种穿刺针直径之间的叶片穿透力在同一生活型中差异不显著，而在草本分别与灌木和乔木等不同生活型中差异显著（P<0.05），同时0.5 mm直径针的穿透力均大于1.0 mm和2.0 mm。（3）叶片穿透力与叶片角质层厚度、叶片厚度、撕裂力呈显著正相关（P<0.05），与比叶面积呈显著负相关（P<0.05），且以针周长校准的穿透力与叶片功能性状的相关性强于以横截面积校准的穿透力，而不同直径穿刺针作用下的叶片穿透力与叶片性状间的关系无显著差异。因此，穿刺针直径和校准方法的不同对叶片穿透力测定结果均产生差异，不同穿刺针直径测定的差异不会影响穿透机械力与叶片功能性状的相关关系，而不同校准方法测定的差异会影响穿透机械力与叶片功能性状相关关系的强弱。综上所述，在叶片穿透力测试中，选取合适的校准方法和穿刺针直径有利于提高数据的规范化和准确性，在此建议在两种校准方法下首先选用以针周长校准的方法，在3种穿刺针直径中选用0.5 mm测量叶面积小、叶脉密度大且较薄的叶片，对于叶面积大、叶脉密度大且较硬的叶片则选用2.0 mm。

关键词: 穿刺针直径, 叶片穿透力, 叶片功能性状

Abstract:

In order to investigate whether there were differences in the results of leaf force-to-punch under different calibration methods and different puncture needle diameters， and the relationship between the results and leaf functional traits， 30 dicotyledonous species were selected to determine the punching force and the tearing force of the leaves and the functional traits of leaves under three puncture needle diameters of 2.0， 1.0 and 0.5 mm， respectively. The results showed that：（1）the leaf force-to-punch differed significantly（P<0.05） among the three different puncture needle diameters， for the same species when the method calibrated by perimeter， the difference between 0.5 mm and 1.0 mm was 76%， between 0.5 mm and 2.0 mm was 76%， and between 1.0 mm and 2.0 mm was 33%（P<0.05）； however， when calibrated by cross-sectional area， the difference was 66%， 56% and 30%（P<0.05） respectively. （2）There was no significant difference in leaf force to punch between the three puncture needle diameters under different calibration methods in the same life form， but there was significant differences（P<0.05） between herbs and different life form such as shrubs and trees， respectively， while the leaf force-to-punch of 0.5 mm diameter needles was greater than that of 1 and 2 mm needles. （3）Leaf force to punch was significantly positively correlated with leaf cuticle thickness， leaf thickness， and leaf tearing force（P<0.05）， and negatively correlated with specific leaf area（P<0.05）， and the correlation between punching force calibrated by needle circumference and leaf functional traits was stronger than that calibrated by cross-sectional area， whereas there was no significant difference in the correlation between leaf force to punch and leaf traits under the action of different diameters of puncture needles. Therefore， differences in both needle diameter and calibration methods produced differences in the results of leaf force to punch measurements. Differences in needle diameters did not affect the correlation between mechanical punching force and leaf functional traits， whereas differences in calibration methods affected the strength of the correlation between mechanical punching force and leaf functional traits. In conclusion， in the leaf force to punch research， selecting the appropriate calibration method and puncture needle diameter was conducive to improving the standardization and accuracy of the data， it was recommended that the two calibration methods based on the needle perimeter should be selected first， and among the three types of puncture needle diameter， 0.5 mm was selected to measure with small leaf area and dense and thin veins， and 2.0 mm was selected to measure with large leaf area and， dense and stiff veins.

Key words: puncture needle diameter, leaf force-to-punch, leaf functional traits

中图分类号:

S432.9+7

欧美景, 李红燕, 赵秋菊, 李佳蔚. 不同测定方法下叶片穿透力与叶片功能性状之间的关系[J]. 植物研究, 2023, 43(6): 910-922.

Meijing OU, Hongyan LI, Qiuju ZHAO, Jiawei LI. Relationship Between Leaf Force-to-punch and Leaf Functional Traits under Different Measurement Methods[J]. Bulletin of Botanical Research, 2023, 43(6): 910-922.

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植物 Species	科 Family	缩写 Abbreviation	生活型 life form
喜旱莲子草Alternanthera philoxeroides	苋科Amaranthaceae	Ap	草本Herb
积雪草Centella asiatica	伞形科Apiaceae	Ca	草本Herb
狗肝菜Dicliptera chinensis	爵床科Acanthaceae	Dc	草本Herb
飞扬草Euphorbia hirta	大戟科Euphorbiaceae	Eh	草本Herb
五爪金龙Ipomoea cairica	旋花科Convolvulaceae	Ipc	草本Herb
假蒟Piper sarmentosum	胡椒科Piperaceae	Ps	草本Herb
雾水葛Pouzolzia zeylanica	荨麻科Urticaceae	Pz	草本Herb
蟛蜞菊Sphagneticola calendulacea	菊科Asteraceae	Sc	草本Herb
龙葵Solanum nigrum	茄科Solanaceae	Sn	草本Herb
黄鹌菜Youngia japonica	菊科Asteraceae	Yj	草本Herb
米仔兰Aglaia odorata	楝科Meliaceae	Ao	灌木Shrub
光叶子花Bougainvillea glabra	紫茉莉科Nyctaginaceae	Bg	灌木Shrub
花叶假连翘Duranta erecta	马鞭草科Verbenaceae	De	灌木Shrub
红背桂Excoecaria cochinchinensis	大戟科Euphorbiaceae	Ec	灌木Shrub
鹅掌柴Heptapleurum heptaphyllum	五加科Araliaceae	Hh	灌木Shrub
龙船花Ixora chinensis	茜草科Rubiaceae	Ic	灌木Shrub
小驳骨Justicia gendarussa	爵床科Acanthaceae	Jg	灌木Shrub
红花檵木Loropetalum chinense	金缕梅科Hamamelidacee	Lc	灌木Shrub
鸡蛋花Plumeria rubra	夹竹桃科Apocynaceae	Pr	灌木Shrub
海桐Pittosporum tobira	海桐科Pittosporaceae	Pt	灌木Shrub
羊蹄甲Bauhinia purpurea	豆科Fabaceae	Bp	乔木Tree
美丽异木棉Ceiba speciosa	锦葵科Malvaceae	Cs	乔木Tree
龙眼Dimocarpus longan	无患子科Sapindaceae	Dl	乔木Tree
枇杷Eriobotrya japonica	蔷薇科Rosaceae	Ej	乔木Tree
黄花风铃木Handroanthus chrysanthus	紫葳科Bignoniaceae	Hc	乔木Tree
大花紫薇Lagerstroemia speciosa	千屈菜科Lythraceae	Ls	乔木Tree
白兰Michelia alba	木兰科Magnoliaceae	Ma	乔木Tree
人心果Manilkara zapota	山榄科Sapotaceae	Mz	乔木Tree
苹婆Sterculia monosperma	锦葵科Malvaceae	Sm	乔木Tree
小叶榄仁Terminalia neotaliala	使君子科Combretaceae	Tn	乔木Tree

性状 Trait	缩写 Abbreviation	单位 Unit
叶片撕裂力Leaf force to tear	Ft	kN·m^-1
叶片穿透力Leaf force to punch	Fp	kN·m^-1
特定叶片穿透力Specific leaf force to punch	Fps	MN·m^-2
叶片厚度Leaf thickness	TL	μm
叶面积Leaf area	La	cm²
叶片鲜质量Leaf fresh mass	Lfm	g
叶片干质量Leaf dry mass	Ldm	g
比叶面积Specific leaf area	SLA	cm²·g^-1
叶脉密度Leaf vein density	VD	mm·mm^-2
上角质层厚度Adaxial cuticle thickness	AdaTc	μm
下角质层厚度Abaxial cuticle thickness	AbaTc	μm

源 Source	以针周长校准的穿透力Fp			以针横截面积校准的穿透力Fps
源 Source	平方和（自由度） Sum of squares（d_f）	均方 Mean square	F	平方和（自由度） Sum of squares（d_f）	均方 Mean square	F
生活型 Life form	1.291（2）	0.646	39.11^***	14.399（2）	7.199	29.425^***
穿刺针直径 Diameter	0.085（2）	0.042	2.56	1.788（2）	0.894	3.653*
生活型×穿刺针直径 Life form×Diameter	0.010（4）	0.002	0.14	0.149（4）	0.037	0.152
误差Error	1.337（81）	0.017	—	19.818（81）	0.245	—
总计Total	13.787（90）	—	—	247.123（90）	—	—

性状 Traits	Fp			Fps
性状 Traits	1.0 mm&0.5 mm	2.0 mm&0.5 mm	2.0 mm&1 mm	1.0 mm&0.5 mm	2.0 mm&0.5 mm	2.0 mm&1.0 mm
TL	0.54	0.43	0.86	0.46	0.35	0.84
SLA	0.52	0.37	0.86	0.4	0.25	0.81
VD	0.63	0.55	0.9	0.46	0.34	0.84
AdaTc	0.52	0.4	0.86	0.4	0.27	0.82
AbaTc	0.51	0.39	0.86	0.43	0.3	0.83
Ft	0.31	0.13	0.78	0.27	0.12	0.75

不同测定方法下叶片穿透力与叶片功能性状之间的关系

Relationship Between Leaf Force-to-punch and Leaf Functional Traits under Different Measurement Methods

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