土壤石砾含量对掌叶木幼苗生长及生理影响的年际变化

doi:10.7525/j.issn.1673-5102.2023.06.011

植物研究 ›› 2023, Vol. 43 ›› Issue (6): 890-899.doi: 10.7525/j.issn.1673-5102.2023.06.011

土壤石砾含量对掌叶木幼苗生长及生理影响的年际变化

马朝阳¹^,²^,³, 刘天凤¹^,²^,³, 李晓雪¹^,²^,³, 霍灿灿¹^,²^,³, 付玲玲¹^,²^,³, 李在留¹^,²^,³()

^1.广西大学林学院，南宁 530004
^2.国家林草局中南速生材繁育重点实验室点实验室，南宁 530004
^3.广西森林生态与保育重点实验室，南宁 530004

收稿日期:2023-01-31 出版日期:2023-11-20 发布日期:2023-11-08
通讯作者: 李在留 E-mail:lizailiu666@163.com
作者简介:马朝阳（1996—），男，硕士研究生，主要从事植物栽培与养护管理的研究。
基金资助:
广西林业科技推广示范项目(桂林科字［2022］第24号);国家自然科学基金项目(31560200);广西大学“大学生创新创业训练计划”项目(S202210593343)

Interannual Changes in the Effects of Soil Gravel Content on the Growth and Physiology of Handeliodendron bodinieri Seedlings

Chaoyang MA¹^,²^,³, Tianfeng LIU¹^,²^,³, Xiaoxue LI¹^,²^,³, Cancan HUO¹^,²^,³, Lingling FU¹^,²^,³, Zailiu LI¹^,²^,³()

^1.Guangxi University，College of Forestry，Nanning 530004
^2.Key Laboratory of National Forestry and Grassland Administration on Cultivation of Fast-Growing Timber in Central South China，Nanning 530004
^3.Guangxi Key Laboratory of Forest Ecology and Conservation，Nanning 530004

Received:2023-01-31 Online:2023-11-20 Published:2023-11-08
Contact: Zailiu LI E-mail:lizailiu666@163.com
About author:E-mail：lizailiu666@163.com
MA Chaoyang（1996—），male，postgraduate，research interests are in plant cultivation and conservation management.
Supported by:
Guangxi Forestry Science and Technology Promotion Demonstration Project（Guilin Kezi［2022］ No.24）;National Natural Science Foundation of China(31560200);Guangxi University“Innovation and Entrepreneurship Training Program for College Students”(S202210593343)

摘要/Abstract

摘要：

掌叶木（Handeliodendron bodinieri）是我国西南喀斯特地区特有濒危物种，为探究土壤石砾含量对不同生长季掌叶木幼苗生长及生理特性的影响，以1年生掌叶木实生苗为试验材料，设置不同土壤石砾含量（0<ck>、20%、40%、60%、80%），开展盆栽试验，测定3个连续生长季内各组幼苗生长、光合及抗性生理指标，并进行综合评价。结果表明：在第1个生长季，当土壤石砾含量为60%时，幼苗净光合速率显著高于对照组；当土壤石砾含量为40%时，幼苗地径增长率、叶生物量、蒸腾速率、气孔导度、可溶性蛋白质量分数及超氧化物歧化酶（SOD）活性显著高于对照组；幼苗胞间CO₂摩尔分数和可溶性糖质量分数在石砾含量大于20%时显著高于对照组；株高增长率、叶面积及根、茎、总生物量与对照组差异不显著。在第2个生长季，当土壤石砾含量为60%时，掌叶木幼苗蒸腾速率及可溶性蛋白质量分数显著高于对照组；当土壤石砾含量为40%时，幼苗SOD活性显著高于对照组，茎生物量在土壤石砾含量为20%时显著高于对照组，其余指标与第1生长季趋势相似。在第3个生长季，掌叶木幼苗株高和地径增长率、茎生物量、叶面积、气孔导度、SOD和过氧化物酶（POD）活性均变为在土壤石砾含量为60%时达到最大值，幼苗净光合速率、胞间CO₂摩尔分数变为在土壤石砾含量为40%时达到最大值，均显著高于对照组。综合分析表明，3个生长季内，掌叶木幼苗得分最高的土壤石砾含量分别为40%、40%、60%，对于连续3个生长季，幼苗在土壤石砾含量60%时生长状况最好。

关键词: 掌叶木幼苗, 土壤石砾含量, 生长季, 生长及生理特性

Abstract:

Handeliodendron bodinieri is an endangered species endemic in karst areas of southwest China. In order to explore the influence of soil gravel content on the growth and physiological characteristics of H. bodinieri seedlings in three consecutives growing seasons， annual H. bodinieri seedlings were used as experimental materials， different soil gravel content（0（control）， 20%， 40%， 60%， 80%） were set， and the growth， photosynthesis and physiological indicators of each group of seedlings were determined and evaluated in three consecutive growth seasons. The results showed that in the first growing season， when the soil gravel content was 60%， the net photosynthetic rate of the seedlings was significantly higher than that of the control group； When the soil gravel content was 40%， the growth rate of seedling diameter， leaf biomass， transpiration rate， stomatal conductance， soluble protein mass fraction， and superoxide dismutase（SOD） activity were significantly higher than those of the control group； The intercellular CO₂ molar fraction and soluble sugar mass fraction of seedlings were significantly higher than those of the control group when the gravel content was greater than 20%； There was no significant difference in plant height growth rate， leaf area， root， stem， and total biomass between the control group and the control group. In the second growing season， when the soil gravel content was 60%， the transpiration rate and soluble protein mass fraction of H. bodinieri seedlings were significantly higher than those of the control group； When the soil gravel content was 40%， the SOD activity of the seedlings was significantly higher than the control group， and the stem biomass was significantly higher than the control group when the soil gravel content was 20%. The other indicators showed similar trends to the first growth season. With the extension of the growing season， in the third growing season， the growth rate of plant height and ground diameter， stem biomass， leaf area， stomatal conductance， SOD and POD activities of H. bodinieri seedlings reached the maximum when the soil gravel content was 60%， which was significantly higher than that of the control group； The net photosynthetic rate and intercellular CO₂ concentration of seedlings reached the maximum when the soil gravel content was 40%， which was significantly higher than that of the control group. The comprehensive analysis showed that in the three consecutive growth seasons， the soil gravel content with the highest score of H. bodinieri seedlings was 40%， 40% and 60% respectively. For three consecutive growth seasons， seedlings grew best at 60% soil gravel content.

Key words: Handeliodendron bodinieri seedlings, soil gravel content, growing season, growth and physiological characteristics

中图分类号:

S794.9

马朝阳, 刘天凤, 李晓雪, 霍灿灿, 付玲玲, 李在留. 土壤石砾含量对掌叶木幼苗生长及生理影响的年际变化[J]. 植物研究, 2023, 43(6): 890-899.

Chaoyang MA, Tianfeng LIU, Xiaoxue LI, Cancan HUO, Lingling FU, Zailiu LI. Interannual Changes in the Effects of Soil Gravel Content on the Growth and Physiology of Handeliodendron bodinieri Seedlings[J]. Bulletin of Botanical Research, 2023, 43(6): 890-899.

图/表 5

图1

图2

图3

表1

掌叶木幼苗各指标主成分分析的因子载荷

指标 Index	处理时间 Processing time
	第一生长季 First growing season				第二生长季 Second growing season				第三生长季 Third growing season
	F₁	F₂	F₃	F₄	F₁	F₂	F₃	F₄	F₁	F₂	F₃	F₄
总株生物量 total plant biomass	0.973	0.077	-0.099	0.043	0.992	0.065	0.005	-0.007	0.936	-0.258	0.040	0.070
蒸腾速率 Transpiration rate	0.374	0.703	0.239	-0.452	-0.654	0.571	0.243	0.197	0.824	0.025	0.293	-0.152
叶生物量 Leaf biomass	0.901	-0.011	0.219	0.226	0.767	0.096	-0.334	-0.144	0.815	-0.304	-0.217	0.025
叶面积 Leaf area	0.819	0.213	-0.249	0.087	0.652	0.265	-0.354	0.411	0.807	0.463	0.188	0.198
气孔导度 Stomatal conductance	0.432	0.569	0.250	-0.549	0.303	0.776	0.391	-0.079	0.880	0.284	-0.296	0.058
株高增长率 Seedling height growth rate	0.451	0.424	0.532	0.522	0.555	0.626	-0.385	0.010	0.132	0.955	0.051	-0.131
可溶性糖质量分数 Soluble sugar content	-0.613	0.681	0.150	0.268	-0.542	0.685	-0.156	-0.382	0.012	0.834	0.174	0.057
可溶性蛋白质量分数 Soluble protein content	-0.558	0.644	0.047	0.117	-0.054	0.861	0.067	0.019	0.642	0.159	0.302	0.316
净光合速率 Net photosynthetic rate	0.084	0.458	-0.656	0.498	-0.239	0.613	-0.280	0.396	0.712	0.302	-0.433	0.218
茎生物量 Stem biomass	0.660	-0.228	0.188	0.566	0.717	0.323	0.004	0.279	0.762	-0.574	0.124	0.085
根生物量 Root biomass	0.923	0.153	-0.232	-0.130	0.975	-0.036	0.120	-0.047	-0.105	-0.895	0.118	0.056
根冠比 Root shoot ratio	0.723	0.232	-0.416	-0.382	0.786	-0.297	0.369	-0.075	0.624	-0.203	-0.542	-0.047
地径增长率 Ground diameter growth rate	0.661	0.386	0.413	-0.167	0.861	0.423	-0.016	-0.018	0.633	-0.050	0.368	-0.517
胞间CO₂摩尔分数 Intercellular CO₂ concentration	-0.574	-0.153	0.699	-0.095	-0.001	0.388	0.837	0.228	0.278	0.829	-0.243	-0.199
超氧化物歧化酶活性 SOD activity	-0.538	0.585	0.187	0.101	0.069	0.482	-0.038	-0.654	-0.422	0.362	-0.735	0.280
过氧化物酶活性 POD activity	-0.508	0.601	-0.414	0.319	-0.816	0.108	-0.173	0.376	-0.167	0.885	0.224	-0.208
丙二醛质量摩尔浓度 MDA content	-0.688	0.118	-0.362	-0.491	-0.718	0.317	-0.075	-0.218	0.936	-0.258	0.040	0.070
特征值 Eigenvalue	7.253	3.156	2.237	2.046	7.188	3.860	1.593	1.292	6.262	5.046	2.021	1.084
贡献率 contribution values/%	42.663	18.567	13.158	12.037	42.285	22.708	9.370	7.602	36.838	29.682	11.891	6.377
累计贡献率 Accumulated contribution rate/%	42.663	61.230	74.388	86.425	42.285	64.992	74.362	81.964	36.838	66.520	78.411	84.787

表1

表2

参考文献 27

1	刘红弟，宋杨，张红军.越桔园土壤有效养分和叶片营养状况分析［J］.中国南方果树，2016，45（6）：32-38.
	LIU H D， SONG Y， ZHANG H J.Analysis on nutrient element contents of soils and leaves in blueberry orchards［J］.South China Fruits，2016，45（6）：32-38.
2	SHI Z J， XU L H， WANG Y H，et al.Effect of rock fragments on macropores and water effluent in a forest soil in the stony mountains of the Loess Plateau，China［J］.African Journal of Biotechnology，2012，11（39）：9350-9361
3	MI M X， SHAO M A， LIU B X.Effect of rock fragments content on water consumption，biomass and water-use efficiency of plants under different water conditions［J］.Ecological Engineering，2016，94：574-582.
4	薛建辉，周之栋，吴永波.喀斯特石漠化山地退化土壤生态修复研究进展［J］.南京林业大学学报（自然科学版），2022，46（6）：135-145.
	XUE J H， ZHOU Z D， WU Y B.Research progresses on ecological remediation of the degraded soil in Karst rocky desertification mountainous areas［J］.Journal of Nanjing Forestry University（Natural Sciences Edition），2022，46（6）：135-145.
5	何鑫，钟九生，林双双，等.西南喀斯特地区耕地破碎化与石漠化空间格局及耦合关系研究［J］.西南大学学报（自然科学版），2022，44（6）：160-170.
	HE X， ZHONG J S， LIN S S，et al.Study on spatial pattern and coupling relationship between fragmentation and rocky desertification of cultivated land in southwestern karst area［J］.Journal of Southwest University（Natural Science Edition），2022，44（6）：160-170.
6	黄仕训，骆文华，唐文秀，等.石山稀有濒危植物迁地保护适应性研究（简报）［J］.广西植物，2002，22（2）：136-139.
	HUANG S X， LUO W H， TANG W X，et al.Study on the adaptability of the rare and threatened limestone plants ex-situ conservation［J］.Guihaia，2002，22（2）：136-139.
7	郭松，李在留，薛建辉，等.掌叶木不同种源种子的经济性状［J］.林业科学，2019，55（4）：84-96.
	GUO S， LI Z L， XUE J H，et al.Economic properties of seeds of Handeliodendron bodinieri from different provenances［J］.Scientia Silvae Sinicae，2019，55（4）：84-96.
8	XIE C， LIU T F， GUO S，et al.Effects of ultra-dry storage on seed germination and seedling growth of Handeliondendron bodinieri ［J］.Silva Fennica，2021，55（3）：10509.
9	李在留，李雪萍，郭松，等.珍稀濒危植物掌叶木的开花生物学特性与繁育系统［J］.园艺学报，2015，42（2）：311-320.
	LI Z L， LI X P， GUO S，et al.Flowering biology and breeding system of the rare and endangered plant Handeliodendron bodinieri ［J］.Acta Horticulturae Sinica，2015，42（2）：311-320.
10	吕清华，程菊英，罗四莲，等.一种值得注意的油脂植物：掌叶木［J］.广西植物，1980（1）：64-65.
	LÜ Q H， CHEN J Y， LUO S L，et al.A kind of oil plant worth attention：Handeliodendron bodinieri ［J］.Guihaia，1980（1）：64-65.
11	LIU X T， LIU T F， ZHANG C，et al.Transcriptome profile analysis reveals the regulation mechanism of stamen abortion in Handeliodendron bodinieri ［J］.Forests，2021，12（8）：1071.
12	谢川，李晓雪，郭松，等.珍稀濒危植物掌叶木的研究动态［J］.分子植物育种，2020，18（5）：1725-1730.
	XIE C， LI X X， GUO S，et al.Research trends of the rare and endangered plant Handeliodendron bodinieri ［J］.Molecular Plant Breeding，2020，18（5）：1725-1730.
13	李晓雪.不同含石量土壤对掌叶木幼苗生长及生理特性的影响［D］.南宁：广西大学，2020.
	LI X X.The influence of different mass percent of stone on the growth and physiological characteristics of Handeliodendron bodinieri seedings［D］.Nanning：Guangxi University，2020.
14	刘天凤，谢川，郭松，等.土壤石砾含量对掌叶木幼苗生长和根系特征的影响［J］.广西植物，2022，42（7）：1240-1247.
	LIU T F， XIE C， GUO S，et al.Influence of gravel content of soil on growth and root characteristics of Handeliodendron bodinieri seedlings［J］.Guihaia，2022，42（7）：1240-1247.
15	陈建勋，王晓峰.植物生理学实验指导［M］.广州：华南理工大学出版社，2006.
	CHEN J B， WANG X F.Experimental guidance on plant physiology［M］.Guangzhou：South China University of Technology Press，2006.
16	顾云春.中国国家重点保护野生植物现状［J］.中南林业调查规划，2003，22（4）：1-7.
	GU Y C.Status quo of China's state priority protected wild plants［J］.Central South Forest Inventory and Planning，2003，22（4）：1-7.
17	NI J， LUO D H， XIA J，et al.Vegetation in karst terrain of southwestern China allocates more biomass to roots［J］.Solid Earth，2015，6（3）：799-810.
18	YE H L， CHEN Z G， JIA T T，et al.Response of different organic mulch treatments on yield and quality of Camellia oleifera ［J］.Agricultural Water Management，2021，245：106654.
19	高歌，李正才，葛晓改，等.施氮对干旱胁迫下毛竹幼苗生物量和根系形态的影响［J］.生态学杂志，2022，41（5）：858-864.
	GAO G， LI Z C， GE X G，et al.Effects of nitrogen addition on biomass and root morphology of Phyllostachys edulis seedlings under drought stress［J］.Chinese Journal of Ecology，2022，41（5）：858-864.
20	贾婷婷，常伟，范晓旭，等.盐胁迫下AM真菌对沙枣苗木光合与叶绿素荧光特性的影响［J］.生态学报，2018，38（4）：1337-1347.
	JIA T T， CHANG W， FAN X X，et al.Effects of Arbuscular mycorrhizal fungi on photosynthetic and chlorophyll fluorescence characteristics in Elaeagnus angustifolia seedlings under salt stress［J］.Acta Ecologica Sinica，2018，38（4）：1337-1347.
21	王延双，方文，王欣彤，等.水淹胁迫对红花玉兰苗木生长和生理生化特性的影响［J］.北京林业大学学报，2020，42（1）：35-45.
	WANG Y S， FANG W， WANG X T，et al.Effects of waterlogging stress on growth，physiological and piochemistry characteristics of Magnolia wufengensis ［J］.Journal of Beijing Forestry University，2020，42（1）：35-45.
22	崔振，李昌晓，贺燕燕，等.中华金叶榆和银水牛果苗木的生长和光合作用对土壤锌污染的响应［J］.林业科学，2017，53（9）：114-122.
	CUI Z， LI C X， HE Y Y，et al.Responses of growth，photosynthesis of Ulmus pumila ‘Jinye' and Shepherdia argentea to soil zinc contamination［J］.Scientia Silvae Sinicae，2017，53（9）：114-122.
23	LO’AY A A， MOSTAFA N A， AL-QAHTANI S M，et al.Influence of the position of mango fruit on the tree（Mangifera indica L.cv.‘Zibda'） on chilling sensitivity and antioxidant enzyme activity［J］.Horticulturae，2021，7（12）：515-532.
24	何榜眼，刘世男，杨梅，等.低磷胁迫对大花序桉幼苗叶片生理指标的影响［J］.西南农业学报，2022，35（2）：418-424.
	HE B Y， LIU S N， YANG M，et al.Effects of low phosphorus stress on physiological characteristics of Eucalyptus coleziana seedling leaves［J］.Southwest China Journal of Agricultural Sciences，2022，35（2）：418-424.
25	王佳琪，崔东洁，阴悦，等.大气压低温等离子体处理对拟南芥幼苗抗氧化系统的影响［J］.植物生理学报，2020，56（3）：423-430.
	WANG J Q， CUI D J， YIN Y，et al.Effect of cold atmospheric pressure plasmas treatment on antioxidant system in Arabidopsis thaliana seedlings［J］.Plant Physiology Journal，2020，56（3）：423-430.
26	谢东锋，张光灿，夏宣宣，等.不同浓度酚酸对欧美杨I-107苗木生长和光合特性的影响［J］.生态学报，2018，38（5）：1789-1798.
	XIE D F， ZHANG G C， XIA X X，et al.Effects of phenolic acids on the growth and photosynthesis of Populus × euramericana ‘Neva’［J］.Acta Ecologica Sinica，2018，38（5）：1789-1798.
27	BALIC I， VIZOSO P， NILO-POYANCO R，et al.Transcriptome analysis during ripening of table grape berry cv.Thompson seedless［J］.PLoS One，2018，13（1）：e0190087.

处理时间 Processing time	土壤石砾含量 Soil gravel content/%	F₁	F₂	F₃	F₄	综合得分 Comprehensive score	综合排名 Comprehensive ranking
第一生长季 First growing season	0	1.195	-1.936	-1.539	-1.236	-0.201	4
	20	0.542	-1.555	2.283	0.472	0.300	2
	40	2.965	2.368	0.358	-0.709	1.667	1
	60	-0.181	0.272	-1.367	2.190	0.057	3
	80	-4.522	-6.532	0.265	-0.718	-3.194	5
第二生长季 Second growing season	0	0.399	-3.087	-0.383	0.803	-0.507	4
	20	1.154	-0.592	1.511	-0.128	0.486	3
	40	3.541	1.412	-0.467	-0.906	1.705	1
	60	1.225	1.995	-0.602	1.225	1.008	2
	80	-4.174	0.271	-0.058	-0.995	-1.784	5
第三生长季 Third growing season	0	-2.228	-2.932	-1.070	-0.230	-1.833	4
	20	0.077	-1.019	0.253	0.862	-0.189	3
	40	3.007	-0.870	1.516	-0.603	0.991	2
	60	2.177	2.148	-1.593	0.505	1.282	1
	80	-3.033	2.673	0.894	-0.534	-0.251	5
连续3个生长季 Three consecutive growing seasons	0	-0.067	0.610	-1.541	-0.671	-0.340	4
	20	1.231	-1.199	0.191	-0.459	0.060	3
	40	0.152	0.113	-0.211	1.766	0.380	2
	60	0.234	1.254	1.185	-0.412	0.570	1
	80	-1.550	-0.778	0.376	-0.225	-0.670	5

土壤石砾含量对掌叶木幼苗生长及生理影响的年际变化

Interannual Changes in the Effects of Soil Gravel Content on the Growth and Physiology of Handeliodendron bodinieri Seedlings

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